Projects

IDENTIFICATION, CHARACTERIZATION, AND BIOLOGY OF FOREIGN AND EMERGING VIRAL AND BACTERIAL PLANT PATHOGENS Appropriated (D)   Accession Number: 422773

MASSIVELY PARALLEL SEQUENCING AS A DIAGNOSTIC AND FORENSIC TOOL FOR PLANT PATHOGENS Reimbursable (R)   Accession Number: 418795

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Molecular analysis of Soybean dwarf virus isolates in the eastern United States confirms the presence of both D and Y strains and provides evidence of mixed infections and recombination - (Peer Reviewed Journal)

Schneider, W.L., Damsteegt, V.D., Stone, A.L., Kuhlman, M., Bunyard, B., Sherman, D.J., Graves, M., Smythers, G., Smith, O., Hatziloukas, E. 2011. Molecular analysis of Soybean dwarf virus isolates in the eastern United States confirms the presence of both D and Y strains and provides evidence of mixed infections and recombination. Virology. 412: 46-54.

Molecular, ultrastructural and biological characterization of Pennsylvania isolates of plum pox potyvirus (PPV) - (Peer Reviewed Journal)

Schneider, W.L., Damsteegt, V.D., Gildow, F.E., Stone, A.L., Sherman, D.J., Levy, L.E., Mavrodieva, V., Richwine, N., Welliver, R., Luster, D.G. 2011. Molecular, ultrastructural and biological characterization of Pennsylvania isolates of plum pox potyvirus (PPV). Phytopathology. 94:528-533.

Acquistion and transmissibility of United States Soybean dwarf virus isolates by the soybean aphid Aphis glycines - (Peer Reviewed Journal)

Damsteegt, V.D., Stone, A.L., Kuhlmann, M., Gildow, F.E., Domier, L.L., Sherman, D.J., Tian, B., Schneider, W.L. 2011. Acquistion and transmissibility of United States Soybean dwarf virus isolates by the soybean aphid Aphis glycines. Plant Disease. 95: 945-950.

Analysis of visual symptomatology in peach and plum inoculated with U.S. PPV isolates - (Abstract Only)

Younkins, A.A., Stone, A.L., Sherman, D.J., Schneider, W.L., Scorza, R., Damsteegt, V.D. 2010. Analysis of visual symptomatology in peach and plum inoculated with U.S. PPV isolates. Phytopathology. 100:S209.

Development of primers and probes for detection of citrus "Candidatus Liberibacter species" by real-time PCR - (Abstract Only)

Govindarajulu, A., Choudhary, N., Hartung, J.S., Schneider, W.L., Brlansky, R.H. 0200. Development of primers and probes for detection of citrus "Candidatus Liberibacter species" by real-time PCR. Phytopathology 100(6):S42.

The relevance of Murraya paniculata and related species as potential hosts and inoculum reservoirs of Candidatus Liberibacter asiaticus, causal agent of Huanglongbing (HLB) - (Peer Reviewed Journal)

Damsteegt, V.D., Postnikova, E.N., Stone, A.L., Kuhlmann, M., Wilson, C., Sechler, A.J., Schaad, N.W., Brlansky, R.H., Schneider, W.L. 2010. The relevance of Murraya paniculata and related species as potential hosts and inoculum reservoirs of Candidatus Liberibacter asiaticus, causal agent of Huanglongbing (HLB). Plant Disease. 94:528-533.

An improved method of DNA extraction from Diaphorina citri for HLB detection - (Abstract Only)

Postnikova, E.N., Stone, A.L., Wilson, C.M., Sherman, D.J., Sechler, A.J., Schuenzel, E., Schaad, N.W., Schneider, W.L., Damsteegt, V.D. 2009. An improved method of DNA extraction from Diaphorina citri for HLB detection. Phytopathology. 99:S104.

Preliminary evidence for mixed populations of Ca. Liberibacter species in Huanglongbing infections - (Abstract Only)

Postnikova, E.N., Stone, A.L., Wilson, C.M., Sherman, D.J., Sechler, A.J., Schuenzel, E., Schaad, N.W., Schneider, W.L., Damsteegt, V.D. 2009. Preliminary evidence for mixed populations of Ca. Liberibacter species in Huanglongbing infections. Phytopathology. 99:S205.

Rathayibacter iranicus isolated from asymptomatic wheat seeds in Turkey - (Peer Reviewed Journal)

Postnikova, E.N., Agarkova, I., Altundag, S., Eskandari, F., Sechler, A.J., Karahan, A., Vidaver, A.K., Schneider, W.L., Ozakman, M., Schaad, N.W. 2009. Rathayibacter iranicus isolated from asymptomatic wheat seeds in Turkey. Plant Pathology. 58:796.

Cultivation of Candidatus Liberibacter asiaticus and Ca. L. americanus associated with Huanglongbing - (Peer Reviewed Journal)

Sechler, A.J., Schuenzel, E., Cooke, P.H., Donnua, S., Thaveechai, N., Postnikova, E.N., Stone, A.L., Schneider, W.L., Damsteegt, V.D., Schaad, N.W. 2009. Cultivation of Candidatus Liberibacter asiaticus and Ca. L. americanus associated with Huanglongbing. Phytopathology. 99:480-486.

Identification of bacterial plant pathogens using multilocus PCR and electrospray ionization-mass spectrometry (PCR/ESI-MS) - (Peer Reviewed Journal)

Postnikova, E.N., Baldwin, C., Whitehouse, C., Sechler, A.J., Schaad, N.W., Sampath, R., Harpin, V., Li, F., Melton, R., Blynn, L., Drader, J., Hofstadler, S., Schneider, W.L. 2008. Identification of bacterial plant pathogens using multilocus PCR and electrospray ionization-mass spectrometry (PCR/ESI-MS). Phytopathology. 98-11-1156-1164

Fidelity of a viral RNA polymerase in an intact host: template structure and host factors affect rates of deletion mutations - (Peer Reviewed Journal)

Pita, J., De Miranda, J., Schneider, W.L., Roossinck, M.J. 2007. Fidelity of a viral RNA polymerase in an intact host: template structure and host factors affect rates of deletion mutations. Journal of Virology. 81:9072-9077.

IDENTIFICATION OF A MUTATION IN THE PLUM POX POTYVIRUS NIB GENE ASSOCIATED WITH ADAPTATION TO PEA (PISUM SATIVUM) - (Peer Reviewed Journal)

Wallis, C.M., Schneider, W.L., Stone, A.L., Sherman, D.J., Damsteegt, V.D., Gildow, F.E. 2007. Identification of a mutation in the plum pox potyvirus nib gene associated with adaptation to pea (pisum sativum). Journal of General Virology. 88:2839-2845.

Murraya paniculata (orange jasmine), a host and possible inoculum reservoir for Candidatus Liberibacter asiaticus, causal agent of Huanglongbing - (Abstract Only)

Damsteegt, V.D., Schneider, W.L., Stone, A.L. 2007. Murraya paniculata (orange jasmine), a host and possible inoculum reservoir for Candidatus Liberibacter asiaticus, causal agent of Huanglongbing. Phytopathology. 97:S26 . Phytopathology.

A comprehensive Prunus pathogen array - (Abstract Only)

Schneider, W.L., Sherman, D.J. 2007. A comprehensive Prunus pathogen array. Phytopathology. 97:S105

PRUNUS HOST RANGE OF PLUM POX VIRUS (PPV) IN THE UNITED STATES BY APHID AND GRAFT INOCULATION - (Peer Reviewed Journal)

Damsteegt, V.D., Scorza, R., Stone, A.L., Schneider, W.L., Webb, K.K., Demuth, M.A., Gildow, F.E. 2007. Prunus host range of plum pox virus (ppv) in the United States by aphid and graft inoculation. Plant Disease. 91:18-23

IDENTIFICATION, CHARACTERIZATION AND USE OF A BROME MOSAIC VIRUS STRAIN AS A VECTOR FOR GENE SILENCING IN MONOCOTYLEDONOUS HOSTS - (Peer Reviewed Journal)

Ding, X.S., Schneider, W.L., Chaluvadi, S., Mian, M.R., Nelson, R.S. 2006. Identification, characterization and use of a brome mosaic virus strain as a vector for gene silencing in monocotyledonous hosts. Molecular Plant-Microbe Interactions. MPMI 19:1229-1239

ANALYSIS OF GENETIC BOTTLENECKS DURING HORIZONTAL TRANSMISSION OF CUCUMBER MOSAIC VIRUS - (Peer Reviewed Journal)

Ali, A., Li, H., Schneider, W.L., Sherman, D.J., Gray, S.M., Smith, D., Roossinck, M.J. 2006. Analysis of genetic bottlenecks during horizontal transmission of cucumber mosaic virus. Journal of Virology. 80:8345-8350

Synthesis of minus-strand copies of a viral transgene during viral infections of transgenic plants - (Peer Reviewed Journal)

Deng, M., Schneider, W.L., Allison, R. 2006. Synthesis of minus-strand copies of a viral transgene during viral infections of transgenic plants. Virus Research.10.1016/ 2006.05.009.

QUANTIFICATION OF POSITIVE AND NEGATIVE STRAND RATIOS FOR PLUM POX VIRUS BY REAL-TIME FLUORESCENT REVERSE TRANSCRIPTION-PCR - (Peer Reviewed Journal) - (10-Dec-05)

PLUM POX POTYVIRUS POPULATION DIVERSITY IN PENNSYLVANIA, IN SINGLE ORCHARDS, AND IN INDIVIDUAL REPLICATING POPULATIONS - (Other)

Schneider, W.L., Sherman, D.J., Stone, A.L., Buckley, K., Damsteegt, V.D. 2005. Plum pox potyvirus population diversity in Pennsylvania, in single orchards, and in individual replicating populations [abstract]. Phytopathology. 95:S94.

THE SOYBEAN APHID, APHIS GLYCINES, A NEW VECTOR OF ENDEMIC DWARFING AND YELLOWING ISOLATES OF SOYBEAN DWARF LUTEOVIRUS - (Abstract Only)

Damsteegt, V.D., Stone, A.L., Schneider, W.L., Sherman, D.J., Gildow, F.E., Luster, D.G. 2005. The soybean aphid, Aphis glycines, a new vector of endemic dwarfing and yellowing isolates of soybean dwarf luteovirus [abstracr]. Phytopathology. 95:S22.

IN PLANTA INTERACTIONS OF THREE PLUM POX POTYVIRUS STRAINS WITHIN CULTIVAR LOVELL PEACH SEEDLINGS - (Abstract Only)

Damsteegt, V.D., Stone, A.L., Sherman, D.J., Schneider, W.L., Gildow, F., Luster, D.G. 2005. In planta interactions of three plum pox potyvirus strains within cultivar Lovell peach seedlings[abstract]. Phytopathology. 95:S22.

TRANSMISSION OF THREE NORTH AMERICAN ISOLATES OF PLUM POX VIRUS: IDENTIFICATION OF APHID VECTORS AND SPECIES-SPECIFIC TRANSMISSION FROM INFECTED STONE FRUITS - (Proceedings)

Gildow, F., Damsteegt, V.D., Stone, A.L., Schneider, W.L., Luster, D.G., Levy, L. 2004. Transmission of three north american isolates of plum pox virus: identification of aphid vectors and species-specific transmission from infected stone fruits. Acta Horticulture 657:207-212

POTENTIAL PRUNUS HOST RANGE OF PPV-PENN ISOLATES BY APHID TRANSMISSION - (Proceedings)

Damsteegt, V.D., Stone, A.L., Gildow, F.E., Schneider, W.L., Luster, D.G. 2004. Potential prunus host range of ppv-penn isolates by aphid transmission. Acta Horticulture 657:201-206

TRANSMISSION OF TWO NORTH AMERICAN ISOLATES OF PLUM POX VIRUS: IDENTIFICATION OF APHID VECTORS AND SPECIES-SPECIFIC TRANSMISSION FROM INFECTED STONE FRUITS - (Proceedings)

Gildow, F.E., Damsteegt, V.D., Stone, A.L., Schneider, W.L., Luster, D.G., Levy, L. 2004. Transmission of two north american isolates of plum pox virus: identification of aphid vectors and species-specific transmission from infected stone fruits. Acta Horticulture 657:207-212

DETECTION AND QUANTIFICATION OF PLUM POX POTYVIRUS IN APHID VECTORS BY REAL-TIME FLUORESCENT REVERSE TRANSCRIPTION-PCR - (Peer Reviewed Journal)

Acta Horticulture 657:135-140

PLUM POX IN NORTH AMERICA: IDENTIFICATION OF APHID VECTORS AND A ROLE FOR FRUIT IN VIRUS SPREAD - (Peer Reviewed Journal)

Gildow, F., Damsteegt, V.D., Stone, A.L., Schneider, W.L., Luster, D.G., Levy, L. 2004. Plum pox in north america: identification of aphid vectors and a role for fruit in virus spread. Phytopathology. 94:868-874.

SPECIFIC DETECTION AND QUANTIFICATION OF PLUM POX POTYVIRUS BY REAL-TIME FLUORESCENT REVERSE TRANSCRIPTION-PCR - (Peer Reviewed Journal)

Schneider, W.L., Sherman, D.J., Stone, A.L., Damsteegt, V.D., Frederick, R.D. 2004. Specific detection and quantification of plum pox potyvirus by real-time fluorescent reverse transcription-PCR. Journal of Virological Methods.120:97-105.

COMPARATIVE HOST RANGE OF U.S. ISOLATES OF PLUM POX VIRUS AMONG PRUNUS AND OTHER WOODY PLANT SPECIES FOLLOWING GRAFT INOCULATION OR APHID TRANSMISSION - (Abstract Only)

Damsteegt, V.D., Scorza, R., Gildow, F.E., Schneider, W.L., Stone, A.L., Luster, D.G. 2004. Comparative host range of U.S. isolates of plum pox virus among Prunus and other woody plant species following graft inoculation or aphid transmission. Phytopathology. 94:S24.

DETECTION AND QUANTIFICATION OF PLUM POX POTYVIRUS DOUBLE-STRANDED RNA WITH REAL-TIME PCR - (Abstract Only)

Schneider, W.L., Stone, A.L., Sherman, D.J., Damsteegt, V.D. 2004. Detection and quantification of plum pox potyvirus double-stranded RNA with real-time PCR. Phytopathology. 94:S93.

HOST ADAPTATION AND MEMORY IN PLUM POX POTYVIRUS POPULATIONS - (Abstract Only)

Schneider, W.L., Stone, A.L., Wallis, C., Sherman, D.J., Shank, K., Damsteegt, V.D., Gildow, F. 2003. Host adaptation and memory in plum pox potyvirus populations. Phytopathology 93:S100.

POTENTIAL PRUNUS HOST RANGE OF PPV-PENN ISOLATES BY APHID TRANSMISSION - (Proceedings)

Damsteegt, V.D., Stone, A.L., Gildow, F.E., Schneider, W.L., Luster, D.G. 2004. Potential prunus host range of ppv-penn isolates by aphid transmission. Acta Horticulture Proceedings. p. 46.

ADVANCES IN MOLECULAR-BASED DIAGNOSTICS IN MEETING CROP BIOSECURITY AND PHYTOSANITARY ISSUES - (Review Article)

SCHAAD, N.W., FREDERICK, R.D., SHAW, J., SCHNEIDER, W.L., HICKSON, R., PETRILLO, M.D., LUSTER, D.G. ADVANCES IN MOLECULAR-BASED DIAGNOSTICS IN MEETING CROP BIOSECURITY AND PHYTOSANITARY ISSUES. ANNUAL REVIEW OF PHYTOPATHOLOGY. 41: . 2003.

SPECIFIC DETECTION AND QUANTIFICATION OF PLUM POX POTYVIRUS BY REAL-TIME FLUORESCENT RT-PCR - (Abstract Only) - (31-Mar-02)

FIELD PERFORMANCE OF CUCURBIT AND TOMATO PLANTS COLONIZED WITH A NONPATHOGENIC, MUTUALISTIC MUTANT (PATH-1) OF COLLETOTRICHUM MAGNA (TELEOMORPH: GLOMERELLA MAGNA; JENKINS AND WINSTEAD) - (Peer Reviewed Journal) - (15-Mar-02)

VIRAL RNA QUASISPECIES GENERATED BY TRANSGENICALLY-EXPRESSED VIRAL REPLICASE - (Abstract Only) - (01-Mar-02)