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United States Department of Agriculture

Agricultural Research Service

John M. Dyer

Acting Research Leader

Biography

Plant Physiology & Genetics Research Unit

USDA-ARS, Arid-Land Agricultural Research Center

   

John M. Dyer

Research Molecular Biologist

Arid-Land Agricultural Research Center

21881 North Cardon Lane

Maricopa, Arizona, USA 85138

520-316-6356 (Office)

520-316-6424 (Lab)

520-316-6330 (FAX)

 

john.dyer@ars.usda.gov

 

Education

 

Post-Doctoral fellow, UT Southwestern Medical Center, Dallas, 1993-1996

Ph.D. Biochemistry, Louisiana State University, 1993

B.A. Chemistry, Louisiana Tech University, 1988

 

Research Interests


My research program focuses on the production of industrially important oils and biofuels in crop plants.  These types of oils represent renewable, environmentally friendly sources of fuel and chemical feedstocks that are typically derived from nonrenewable petroleum.  Experimental approaches include the identification and functional analysis of genes and enzymes involved in the production of industrially important fatty acids (e.g., fatty acids containing hydroxy groups or conjugated double bonds), expression of genes in yeast or plant cell culture to gain insight to the targeting, assembly and post-translational regulation of the encoded enzymes, and metabolic engineering of plants and microbes for production of value-added oils.


Key Publications

 

James, C. Horn, P.J., Richardson, C.C., Gidda, S.K., Zhang, D., Mullen, R.T., Dyer, J.M., Anderson, R.G.W., and Chapman, K.D. (2010). Disruption of the Arabidopsis CGI-58 homologue produces Chanarin–Dorfman-like lipodystrophy in plants. Proc. Natl. Acad. Sci. USA in press. (PDF)

 

O’Quin, J.B., Bourassa, L., Zhang, D., Gidda, S., Foss, S., Shockey, J.M., Chapman, K.D., Mullen, R.T., and Dyer, J.M. (2010). Temperature-sensitive, post-translational regulation of plant omega-3 fatty acid desaturases is mediated by the ER-associated degradation pathway. J. Biol. Chem. 285: 21781-21796. (PDF)

 

Gidda, S.K., Shockey, J.M., Rothstein, S.J., Dyer, J.M., and Mullen, R.T. (2009). Arabidopsis thaliana GPAT8 and GPAT9 are localized to the ER and possess distinct ER retrieval signals: functional divergence of the dilysine ER retrieval motif in plant cells. Plant Physiol. Biochem. 47: 867-879. (PDF)

 

O'Quin, J.B., Mullen, R.T., and Dyer, J.M. (2009). Addition of an N-terminal epitope tag significantly increases the activity of plant fatty acid desaturases expressed in yeast cells. Appl. Microbiol. Biotechnol. 83: 117-125. (PDF)

 

Lessire, R., Cahoon, E., Chapman, K., Dyer, J., Eastmond, P., Heinz, E. (2009). Highlights of recent progress in plant lipid research. Plant Physiol. Biochem. 47: 443-447. (PDF)

 

Slocombe, S.P., Cornah, J., Pinfield-Wells, H., Soady, K., Zhang, Q.Y., Gilday, A., Dyer, J.M., and Graham, I.A. (2009). Oil accumulation in leaves directed by modification of fatty acid breakdown and lipid synthesis pathways. Plant Biotechnol. J.7: 694-703. (PDF)

 

Burgal, J., Shockey, S., Lu, C., Dyer, J., Larson, T., Graham, I., and Browse, J. (2008). Metabolic engineering of hydroxy fatty acid production in plants: RcDGAT2 drives dramatic increases in ricinoleate levels in seed oil. Plant Biotechnol. J. 6: 819-831. (PDF)  

 

Dyer, J.M., Stymne, S., Green, A.G. and Carlsson, A. (2008) High value oils in plants. Plant J. 54: 640-655. (PDF)

 

Lessire, R., Beisson, F., Chapman, K., Dyer, J., and Eastmond, P. (2008). Highlights of the International Symposium on Plant Lipids. Eur. J. Lipid Sci. Technol. 110: 1190-1194. (PDF)

 

Dyer, J.M. and Mullen, R.T. (2008) Engineering plant oils as high-value industrial feedstocks for biorefining: the need for underpinning cell biology research. Physiol. Plantarum 132: 11-22. (PDF)

 

Henderson, M.P., Hwang, Y.T., Dyer, J.M., Mullen, R.T. and Andrews, D.W. (2007) The C-terminus of cytochrome b(5) confers endoplasmic reticulum specificity by preventing spontaneous insertion into membranes. Biochem. J. 401: 701-709. (PDF)

 

Cahoon, E.B., Shockey, J.M., Dietrich, C.R., Gidda, S.K., Mullen, R.T. and Dyer, J.M. (2007) Engineering oilseeds for sustainable production of industrial and nutritional feedstocks: solving bottlenecks in fatty acid flux. Curr. Opin. Plant Biol. 10: 236-244. (PDF)

 

Shockey, J.M., Gidda, S.K., Chapital, D.C., Kuan, J.C., Bland, J.M., Rothstein, S.J., Mullen, R.T. and Dyer, J.M. (2006) Tung tree DGAT1 and DGAT2 have nonredundant functions in triacylglycerol biosynthesis and are localized to different subdomains of the endoplasmic reticulum. Plant Cell 18: 2294-2313. (PDF)

 

Cahoon, E.B., Dietrich, C.R., Meyer, K., Damude, H.G., Dyer, J.M. and Kinney, A.J. (2006) Conjugated fatty acids accumulate to high levels in phospholipids of metabolically engineered soybean and Arabidopsis seeds. Phytochemistry 67: 1166-1176. (PDF)

 

Shrestha, R., Kim, S.C., Dyer, J.M., Dixon, R.A. and Chapman, K.D. (2006) Plant fatty acid (ethanol) amide hydrolases. Biochim. Biophys. Acta 1761: 324-334 (PDF)

 

Dyer, J.M. and Mullen, R.T. (2005) Development and potential of genetically-engineered oilseeds. Seed Sci. Res. 15: 255-267 (PDF)

 

Shockey, J.M., Dhanoa, P.K., Dupuy, T., Chapital, D.C., Mullen, R.T. and Dyer, J.M. (2005) Cloning, functional analysis, and subcellular localization of two isoforms of NADH:cytochrome b(5) reductase from developing seeds of tung (Vernicia fordii). Plant Sci. 169: 375-385. (PDF)

 

Dyer, J.M., Chapital, D.C., Kuan, J.-C.W, Shepherd, H.S., Tang, F. and Pepperman, A.B. (2004) Production of linolenic acid in yeast cells expressing an omega-3 desaturase from tung (Aleurites fordii). J. Am. Oil Chem. Soc. 81: 647-651. (PDF)

 

McCartney, A.W., Dyer, J.M., Dhanoa, P.K., Kim, P.K., Andrews, D.W., McNew, J.A. and Mullen, R.T. (2004) Membrane-bound fatty acid desaturases are inserted co-translationally into the ER and contain different ER retrieval motifs at their carboxy termini. Plant J. 37: 156-173. (PDF)

 

Hwang, Y.T., Pelitire, S.M., Henderson, M.P.A., Andrews, D.W., Dyer, J.M. and Mullen, R.T. (2004) Novel targeting signals mediate the sorting of different isoforms of the tail-anchored membrane protein cytochrome b(5) to either endoplasmic reticulum or mitochondria. Plant Cell 16: 3002-3019. (PDF)

 

Dyer, J.M., Chapital, D.C., Kuan, J.-C., Mullen, R.T. and Pepperman, A.B. (2002) Molecular analysis of a bifunctional fatty acid conjugase/desaturase from tung (Aleurites fordii): Implications for the evolution of plant fatty acid diversity. Plant Physiol. 130: 2027-2038. (PDF)

 

Dyer, J.M., Chapital, D.C., Kuan, J.-C., Mullen, R.T. and Pepperman, A.B. (2002) Metabolic engineering of Saccharomyces cerevisiae for production of novel lipid compounds. Appl. Microbiol. Biotechnol. 59: 224-230. (PDF)

 

Dyer, J.M., Chapital, D.C., Cary, J.W. and Pepperman, A.B. (2001) Chilling-sensitive, post-transcriptional regulation of a plant fatty acid desaturase expressed in yeast. Biochem. Biophys. Res. Comm. 282: 1019-1025. (PDF)

 

Dyer, J.M. and Mullen, R.T. (2001) Immunocytological localization of two plant fatty acid desaturases in the endoplasmic reticulum. FEBS Lett. 494: 44-47. (PDF)


Last Modified: 9/30/2010