Projects

ADVANCED CONVERSION TECHNOLOGIES FOR SUGARS AND BIOFUELS: SUPERIOR FEEDSTOCKS, PRETREATMENTS, INHIBITOR REMOVAL, AND ENZYMES Appropriated (D)   Accession Number: 417041

SUSTAINABLE PRODUCTION AND DISTRIBUTION OF BIOENERGY FOR THE CENTRAL USA Reimbursable (R)   Accession Number: 421659

XYLO-OLIGOSACCHARIDES AND ETHANOL FROM MISCANTHUS: SUSTAINABLE PRODUCTION OF BIOFUELS Specific Cooperative Agreement (S)   Accession Number: 419365

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Conversion of switchgrass to ethanol using dilute ammonium hydroxide pretreatment: influence of ecotype and harvest maturity - (Peer Reviewed Journal) - (20-May-13)

Simultaneous detoxification, saccharification, and ethanol fermentation of weak-acid hydrolyzates - (Peer Reviewed Journal)

Klasson, K.T., Dien, B.S., Hector, R.E. 2013. Simultaneous detoxification, saccharification, and ethanol fermentation of weak-acid hydrolyzates. Industrial Crops and Products. 49:292-298.

Phylogeny-guided screening of yeast strains for lipid production - (Abstract Only)

Dien, B.S., Slininger, P.J., Kurtzman, C.P., Evangelista, R.L., Moser, B.R., Saha, B.C., Cotta, M.A., Balan, V., Jin, M., Sousa, L.D., Dale, B.E. 2013. Phylogeny-guided screening of yeast strains for lipid production [abstract]. Society for Industrial Microbiology and Biotechnology. Poster 8-10.

Comparative lipid production on hydrolyzates of pretreated lignocellulosic biomass using Oleaginous yeasts - (Abstract Only)

Slininger, P.J., Dien, B.S., Kurtzman, C.P., Moser, B.R., Evangelista, R.L., Saha, B.C., Cotta, M.A., Balan, V., Jin, M., Sousa, L.D., Dale, B.E. 2013. Comparative lipid production on hydrolyzates of pretreated lignocellulosic biomass using Oleaginous yeasts [abstract]. Society for Industrial Microbiology and Biotechnology. Poster 8-4.

Production of ethanol and furfural from corn stover - (Abstract Only)

Saha, B.C., Avci, A., Yoshida, T., Dien, B.S., Kennedy, G.J., Cotta, M.A., Sonomoto, K. 2013. Production of ethanol and furfural from corn stover [abstract]. Americal Chemical Society. Paper No. CELL 293.

Use of tropical maize for bioethanol production - (Peer Reviewed Journal) - (09-Mar-13)

Switchgrass biomass composition altered by six generations of divergent breeding for digestibility - (Peer Reviewed Journal)

Vogel, K.P., Mitchell, R., Sarath, G., Jung, H.G., Dien, B.S., Casler, M.D. 2013. Switchgrass biomass composition altered by six generations of divergent breeding for digestibility. Crop Science. 53:853-862. (doi: 10.2135/cropsci2012.09.0542)

Response surface optimization of corn stover pretreatment using dilute phosphoric acid for enzymatic hydrolysis and ethanol production - (Peer Reviewed Journal)

Avci, A., Saha, B.C., Dien, B.S., Kennedy, G.J., Cotta, M.A. 2013. Response surface optimization of corn stover pretreatment using dilute phosphoric acid for enzymatic hydrolysis and ethanol production. Bioresource Technology. 130:603-612.

Comparative analysis of end point enzymatic digests of arabino-xylan isolated from switchgrass (Panicum virgatum L) of varying maturities using LC-MS^n^ - (Peer Reviewed Journal)

Bowman, M.J., Dien, B.S., O Bryan, P.J., Sarath, G., Cotta, M.A. 2012. Comparative analysis of end point enzymatic digests of arabino-xylan isolated from switchgrass (Panicum virgatum L) of varying maturities using LC-MS^n^. Metabolites. 2(4):959-982.

Reduction of fermentation lag phase in biofuel production using a novel activated biochar material - (Proceedings)

Klasson, K.T., Dien, B.S., Hector, R.E. 2012. Reduction of fermentation lag phase in biofuel production using a novel activated biochar material. In: Proceedings of the U.S.-Japan Cooperative Program in Natural Resources Food and Agriculture Panel, USDA-ARS, December 8-13, 2012, Wyndmoor, Pennsylvania. pp. 1-2.

Effect of particle size on enzymatic hydrolysis of pretreated Miscanthus - (Peer Reviewed Journal)

Khullar, E., Dien, B.S., Rausch, K.D., Tumbleson, M.E., Singh, V. 2013. Effect of particle size on enzymatic hydrolysis of pretreated Miscanthus. Industrial Crops and Products. 44:11-17.

Production of xylooligosaccharide (XOS) coproducts from Miscanthus x giganteus - (Abstract Only)

Chen, M., Dien, B.S., Rausch, K.D., Tumbleson, M.E., Singh, V. 2012. Production of xylooligosaccharide (XOS) coproducts from Miscanthus x giganteus. American Association of Cereal Chemists International Meeting.

High titer ethanol production from SPORL-pretreated lodgepole pine by simultaneous enzymatic saccharification and combined fermentation - (Peer Reviewed Journal)

Lan, T.Q., Gleisner, R., Zhu, J.Y., Dien, B.S., Hector, R.E. 2013. High titer ethanol production from SPORL-pretreated lodgepole pine by simultaneous enzymatic saccharification and combined fermentation. Bioresource Technology. 127:291-297.

Shaping reactor microbiomes to produce the fuel precursor n-butyrate from pretreated cellulosic hydrolysates - (Peer Reviewed Journal)

Agler, M.T., Werner, J.J., Iten, L.B., Dekker, A., Cotta, M.A., Dien, B.S., Angenent, L.T. 2012. Shaping reactor microbiomes to produce the fuel precursor n-butyrate from pretreated cellulosic hydrolysates. Environmental Science and Technology. 46(18):10229-10238.

Seashore mallow (Kosteletzkya pentacarpos) as a salt-tolerant feedstock for production of biodiesel and ethanol - (Peer Reviewed Journal)

Moser, B.R., Dien, B.S., Seliskar, D.M., Gallagher, J.L. 2013. Seashore mallow (Kosteletzkya pentacarpos) as a salt-tolerant feedstock for production of biodiesel and ethanol. Renewable Energy. 50:833-839.

Analysis of the cell-wall xylan component of brown midrib mutants of Sorghum bicolor L. by LC-MS^n^ - (Abstract Only)

Bowman, M.J., Dien, B.S., Sattler, S.E. 2012. Analysis of the cell-wall xylan component of brown midrib mutants of Sorghum bicolor L. by LC-MS^n^. Gordon Research Conference [abstract].

Genetically engineered Escherichia coli FBR5: Part I. Comparison of high cell density bioreactors for enhanced ethanol production from xylose - (Peer Reviewed Journal)

Qureshi, N., Dien, B.S., Liu, S., Saha, B.C., Hector, R.E., Cotta, M.A., Hughes, S.R. 2012. Genetically engineered Escherichia coli FBR5: Part I. Comparison of high cell density bioreactors for enhanced ethanol production from xylose. Biotechnology Progress. 28(5):1167-1178.

Genetically engineered Escherichia coli FBR5: Part II. Ethanol production from xylose and simultaneous product recovery - (Peer Reviewed Journal)

Qureshi, N., Dien, B.S., Liu, S., Saha, B.C., Cotta, M.A., Hughes, S.R., Hector, R.E. 2012. Genetically engineered Escherichia coli FBR5: Part II. Ethanol production from xylose and simultaneous product recovery. Biotechnology Progress. 28(5):1179-1185.

Influence of Stenocarpella maydis infected corn on the dry grind ethanol process - (Abstract Only)

Dien, B.S., Wicklow, D.T., Singh, V., Moreau, R.A., Moser, J.K., Cotta, M.A. 2012. Influence of Stenocarpella maydis infected corn on the dry grind ethanol process. 8th Biennial Corn Utilization and Technology Conference.

Screening alfalfa, reed canarygrass, and switchgrass cultivars for ethanol yield by treating with dilute-acid followed by simultaneous saccharification and fermentation - (Abstract Only) - (10-May-12)

Liquid chromatography-mass spectrometry investigation of enzyme-resistant xylooligosaccharide structures of switchgrass associated with ammonia pretreatment, enzymatic saccharification, and fermentation - (Peer Reviewed Journal)

Bowman, M.J., Dien, B.S., Hector, R.E., Sarath, G., Cotta, M.A. 2012. Liquid chromatography-mass spectrometry investigation of enzyme-resistant xylooligosaccharide structures of switchgrass associated with ammonia pretreatment, enzymatic saccharification, and fermentation. Bioresource Technology. 110:437-447.

Influence of Stenocarpella maydis infected corn on the composition of corn kernel and its conversion into ethanol - (Peer Reviewed Journal)

Dien, B.S., Wicklow, D.T., Singh, V., Moreau, R.A., Moser, J.K., Cotta, M.A. 2012. Influence of Stenocarpella maydis infected corn on the composition of corn kernel and its conversion into ethanol. Cereal Chemistry. 89:15-23.

Understanding variation in switchgrass biomass composition - (Popular Publication)

Schmer, M.R., Vogel, K.P., Mitchell, R., Dien, B.S., Jung, H.G., Casler, M.D. 2012. Understanding variation in switchgrass biomass composition. CSA News. March 2012. Pg. 14.

On-farm acidification and anaerobic storage for preservation and improved conversion of switchgrass into ethanol - (Peer Reviewed Journal)

Digman, M.F., Dien, B.S., Hatfield, R.D. 2012. On-farm acidification and anaerobic storage for preservation and improved conversion of switchgrass into ethanol. Biological Engineering (ASABE). 5(1):47-58.

Plant cell walls to ethanol. - (Peer Reviewed Journal)

Jordan, D.B., Bowman, M.J., Braker, J.D., Dien, B.S., Hector, R.E., Lee, C.C., Mertens, J.A., Wagschal, K.C. 2012. Plant cell walls to ethanol. Biochemical Journal. 442:247-252.

Prolonged conversion of n-butyrate to n-butanol with Clostridium saccharoperbutylacetonicum in a two-stage continuous culture with in-situ product removal - (Peer Reviewed Journal)

Richter, H., Qureshi, N., Heger, S., Dien, B.S., Cotta, M.A., Angenent, L.T. 2012. Prolonged conversion of n-butyrate to n-butanol with Clostridium saccharoperbutylacetonicum in a two-stage continuous culture with in-situ product removal. Biotechnology and Bioengineering. 109:913-921.

Nutrient supplementation key to inhibitor-tolerant yeast development and fermentation performance on switchgrass hydrolyzates - (Abstract Only) - (09-Nov-11)

Biodiesel from salt-tolerant seashore mallow (Kosteletzkya virginica) - (Abstract Only)

Moser, B.R., Dien, B.S., Gallagher, J.L. 2012. Biodiesel from salt-tolerant seashore mallow (Kosteletzkya virginica). Annual Meeting and Expo of the American Oil Chemists' Society. p. IOP 5.

Biochemical processing of reed canarygrass into fuel ethanol - (Peer Reviewed Journal)

Dien, B.S., Casler, M.D., Hector, R.E., Iten, L.B., Nichols, N.N., Mertens, J.A., Cotta, M.A. 2012. Biochemical processing of reed canarygrass into fuel ethanol. International Journal of Low-Carbon Technologies. 7:338-347.

Hydrothermal pretreatment of sugarcane bagasse using response surface methodology improves digestibility and ethanol production by SSF - (Peer Reviewed Journal)

da Cruz, S.H., Dien, B.S., Nichols, N.N., Saha, B.C., Cotta, M.A. 2012. Hydrothermal pretreatment of sugarcane bagasse using response surface methodology improves digestibility and ethanol production by SSF. Journal of Industrial Microbiology and Biotechnology. 39:439-447.

Temporal and spatial variation in switchgrass biomass composition and theoretical ethanol yield - (Peer Reviewed Journal)

Schmer, M.R., Vogel, K.P., Mitchell, R., Dien, B.S., Jung, H.G., Casler, M.D. 2012. Temporal and spatial variation in switchgrass biomass composition and theoretical ethanol yield. Agronomy Journal. 104:54-64.

The application of ultrasound in the enzymatic hydrolysis of switchgrass - (Peer Reviewed Journal)

Easson, M.W., Condon, B.D., Dien, B.S., Iten, L.B., Slopek, R.P., Yoshioka-Tarver, M., Lambert, A.H., Smith, J.N. 2011. The application of ultrasound in the enzymatic hydrolysis of switchgrass. Applied Biochemistry and Biotechnology. 165(5):1322-1331.

Long-term conversion of n-butyrate to n-butanol with Clostridium saccharoperbutylacetonicum using a two-stage continuous culture and in-situ product removal - (Abstract Only)

Richter, H., Qureshi, N., Dien, B.S., Cotta, M.A., Angenent, L.T. 2011. Long-term conversion of n-butyrate to n-butanol with Clostridium saccharoperbutylacetonicum using a two-stage continuous culture and in-situ product removal [abstract]. Society for Industrial Microbiology. Paper No. 19556.

Ethanol yields and cell wall properties in divergently bred switchgrass genotypes - (Peer Reviewed Journal)

Sarath, G., Dien, B.S., Saathoff, A.J., Vogel, K.P., Mitchell, R., Chen, H. 2011. Ethanol yields and cell wall properties in divergently bred switchgrass genotypes. Bioresource Technology. 102:9579-9585.

Identifying enzyme resistant xylo-oligomers from processing switchgrass to bioethanol - (Abstract Only)

Bowman, M.J., Dien, B.S., Hector, R.E., Sarath, G., Cotta, M.A. 2011. Identifying enzyme resistant xylo-oligomers from processing switchgrass to bioethanol [abstract]. American Society for Mass Spectrometry. p. 66.

Influence of Stenocarpella maydis infected corn on the dry grind ethanol process - (Abstract Only)

Dien, B.S., Wicklow, D.T., Singh, V., Moreau, R.A., Moser, J.K., Cotta, M.A. 2011. Influence of Stenocarpella maydis infected corn on the dry grind ethanol process [abstract]. International Starch Technology Conference. p. 82.

Influence of Stenocarpella maydis infected corn on the composition of corn kernel and its conversion into ethanol - (Peer Reviewed Journal)

Dien, B.S., Wicklow, D.T., Singh, V., Moreau, R.A., Moser, J.K., Cotta, M.A. 2012. Influence of Stenocarpella maydis infected corn on the composition of corn kernel and its conversion into ethanol. Cereal Chemistry. 89(1):15-23.

Optimizing hydrothermal pretreatment of sugarcane bagasse using response surface methodology - (Abstract Only)

Da Cruz, S., Dien, B.S., Nichols, N.N., Saha, B.C., Cotta, M.A. 2011. Optimizing hydrothermal pretreatment of sugarcane bagasse using response surface methodology [abstract]. In: Proceedings of the Sixth Annual Frontiers in Bioenergy: U.S.-Brazil Symposium on Bioenergy Production, May 15-18, 2011, West Lafayette, Indiana. Paper No. 14.

Dilute ammonium hydroxide pretreatment of reed canary grass and its simultaneous saccharification and fermentation to ethanol using a xylose-fermenting Saccharomyces cerevisiae strain - (Abstract Only)

Dien, B.S., Hector, R.E., Casler, M.D., Cotta, M.A. 2011. Dilute ammonium hydroxide pretreatment of reed canary grass and its simultaneous saccharification and fermentation to ethanol using a xylose-fermenting Saccharomyces cerevisiae strain [abstract]. In: Proceedings of the 33rd Symposium on Biotechnology for Fuels and Chemicals, May 2-5, 2011, Seattle, Washington. Paper No. 4-18.

Switchgrass alkaline pretreatment, enzymatic saccharification, and fermentation with residual oligosaccharide product analysis by mass spectrometry - (Abstract Only)

Bowman, M.J., Dien, B.S., Hector, R.E., Sarath, G., Cotta, M.A. 2011. Switchgrass alkaline pretreatment, enzymatic saccharification, and fermentation with residual oligosaccharide product analysis by mass spectrometry [abstract]. In: Proceedings of the 33rd Symposium on Biotechnology for Fuels and Chemicals, May 2-5, 2011, Seattle, Washington. Paper NO. 10-19.

Culture nutrition key to inhibitor-tolerant yeast performance - (Abstract Only) - (05-May-11)

Temporal and spatial variation in switchgrass biomass composition and theoretical ethanol recovery - (Abstract Only)

Schmer, M.R., Vogel, K.P., Mitchell, R., Dien, B.S., Jung, H.G., Casler, M.D. 2011. Temporal and spatial variation in switchgrass biomass composition and theoretical ethanol recovery [abstract]. ASA-CSSA-SSSA Annual Meeting Abstracts. Abstract No. 64434. Available: http://a-c-s.confex.com/crops/2011am/webprogram/Paper64434.html.

Enhancing alfalfa conversion efficiencies for sugar recovery and ethanol production by altering lignin composition - (Peer Reviewed Journal)

Dien, B.S., Miller, D.J., Hector, R.E., Dixon, R.A., Chen, F., McCaslin, M., Risen, P., Sarath, G., Cotta, M.A. 2011. Enhancing alfalfa conversion efficiencies for sugar recovery and ethanol production by altering lignin composition. Bioresource Technology. 102(11):6479-6486.

Selective chemical oxidation and depolymerization of switchgrass (Panicum virgatum L.) xylan with oligosaccharide product analysis by mass spectrometry - (Peer Reviewed Journal)

Bowman, M.J., Dien, B.S., O Bryan, P.J., Sarath, G., Cotta, M.A. 2011. Selective chemical oxidation and depolymerization of switchgrass (Panicum virgatum L.) xylan with oligosaccharide product analysis by mass spectrometry. Rapid Communications in Mass Spectrometry. 25(8):941-950.

Conversion of starch from dry common beans (Phaseolus vulgaris L.) to ethanol - (Peer Reviewed Journal)

Nichols, N.N., Sutivisedsak, N., Dien, B.S., Biswas, A., Lesch, W.C., Cotta, M.A. 2011. Conversion of starch from dry common beans (Phaseolus vulgaris L.) to ethanol. Industrial Crops and Products. 33(3):644-647.

DOWNREGULATION OF CINNAMYL-ALCOHOL DEHYDROGENASE IN SWITCHGRASS BY RNA SILENCING RESULTS IN ENHANCED GLUCOSE RELEASE AFTER CELLULASE TREATMENT - (Peer Reviewed Journal)

Saathoff, A.J., Sarath, G., Chow, E.K., Dien, B.S., Tobias, C.M. 2011. Downregulation of cinnamyl-alcohol dehydrogenase in switchgrass by RNA silencing results in enhanced glucose release after cellulase treatment. PLoS One. 6(1):e16416. DOI: 10.1371/journal.pone.0016416.

Engineering industrial Saccharomyces cerevisiae strains for xylose fermentation and comparison for switchgrass conversion - (Peer Reviewed Journal)

Hector, R.E., Dien, B.S., Cotta, M.A., Qureshi, N. 2011. Engineering industrial Saccharomyces cerevisiae strains for xylose fermentation and comparison for switchgrass conversion. Journal of Industrial Microbiology and Biotechnology. 38(9):1193-1202.

Use of a microbe to mitigate inhibitors in biomass fermentations - (Abstract Only)

Nichols, N.N., Dien, B.S., Saha, B.C., Cotta, M.A. 2010. Use of a microbe to mitigate inhibitors in biomass fermentations [abstract}. In: Proceedings of the 2010 AIChE Midwest Regional Conference, September 30-October 1, 2010, Chicago, Illinois. p. 34.

Ethanol Production Yield of Five Warm-Season Perennial Forage Grasses - (Abstract Only)

Read, J.J., McLaughlin, M.R., Dien, B.S. 2010. Ethanol Production yield of five warm-season perennial forage frasses. Proceedings Sustainable Feedstock Roadmap Workshop. CD-ROM.

Estimating ethanol yield from switchgrass strains using NIRS - (Abstract Only)

Vogel, K.P., Dien, B.S., Jung, H.G., Casler, M.D., Masterson, S.D., Mitchell, R. 2010. Estimating ethanol yield from switchgrass strains using NIRS. ASA-CSSA-SSSA Annual Meeting Abstracts, 1-3 Nov. 2010, Long Beach, CA. 2010. CDROM Abstract #278-5.

Deactivation of cellulases by phenols - (Peer Reviewed Journal)

Ximenes, E., Kim, Y., Mosier, N., Dien, B.S., Ladisch, M. 2011. Deactivation of cellulases by phenols. Enzyme and Microbial Technology. 48(1):54-60.

Biological Abatement of Fermentation Inhibitors for Ethanol Production from Bioenergy Crops - (Abstract Only) - (27-Aug-10)

Microfiltration of thin stillage: Process simulation and economic analyses - (Peer Reviewed Journal)

Arora, A., Seth, A., Dien, B.S., Belyea, R.L., Singh, V., Tumbleson, M.E., Rausch, K.D. 2011. Microfiltration of thin stillage: Process simulation and economic analyses. Biomass and Bioenergy. 35(1):113-120.

Quantifying actual and theoretical ethanol yields for switchgrass strains using NIRS analyses - (Peer Reviewed Journal)

Vogel, K.P., Dien, B.S., Jung, H.G., Casler, M.D., Masterson, S.D., Mitchell, R. 2011. Quantifying actual and theoretical ethanol yields for switchgrass strains using NIRS analyses. BioEnergy Research. 4(2):96-110. DOI: 10.1007/s12155-010-9104-4.

On-farm Preservation and Pretreatment of Perennial Grasses for Fuel Ethanol Production - (Abstract Only) - (21-May-10)

Fermentation of Bioenergy Crops Into Ethanol Using Biological Abatement for Removal of Inhibitors - (Peer Reviewed Journal)

Nichols, N.N., Dien, B.S., Cotta, M.A. 2010. Fermentation of Bioenergy Crops Into Ethanol Using Biological Abatement for Removal of Inhibitors. Bioresource Technology. 101(19):7545-7550.

Biochemical Conversion of Reduced Lignin Alfalfa Stems Into Ethanol - (Abstract Only) - (22-Apr-10)

Cloning and Expression of Novel Bacterial Xylose Isomerases in Saccharomyces cerevisiae - (Abstract Only)

Hector, R.E., Dien, B.S., Cotta, M.A., Hughes, S.R. 2010. Cloning and expression of novel bacterial xylose isomerases in Saccharomyces cerevisiae. Biotechnology for Fuels and Chemicals Symposium. Abstract #1.

Chemical depolymerization of switchgrass xylan with oligosaccharide product analysis by HPAEC-PAD and mass spectrometry - (Abstract Only)

Bowman, M.J., Dien, B.S., O Bryan, P.J., Sarath, G., Cotta, M.A. 2010. Chemical depolymerization of switchgrass xylan with oligosaccharide product analysis by HPAEC-PAD and mass spectrometry [abstract]. Biotechnology for Fuels and Chemicals. p. 73.

Use of the fungus Coniochaeta ligniaria to remediate fermentation inhibitors from cellulosic sugar streams - (Abstract Only)

Nichols, N.N., Saha, B.C., Dien, B.S., Cotta, M.A. 2010. Use of the fungus Coniochaeta ligniaria to remediate fermentation inhibitors from cellulosic sugar streams [abstract]. Biotechnology for Fuels and Chemicals. Paper No. 11-05.

Pilot-scale On-farm Pretreatment of Perennial Grasses with Dilute Acid and Alkali for Fuel Ethanol Production - (Peer Reviewed Journal)

Digman, M.F., Shinners, K.J., Muck, R.E., Dien, B.S. 2010. Pilot-scale On-farm Pretreatment of Perennial Grasses with Dilute Acid and Alkali for Fuel Ethanol Production. Transactions of the ASABE. 53(3):1007-1014.

Full-scale on-farm pretreatment of perennial grasses with dilute acid for fuel ethanol production - (Peer Reviewed Journal)

Digman, M.F., Shinners, K.J., Muck, R.E., Dien, B.S. 2010. Full-scale on-farm pretreatment of perennial grasses with dilute acid for fuel ethanol production. BioEnergy Research. 3:335-341.

Toward Narrowing Fermentation Endproduct Distribution in Undefined Mixed Culture Anaerobic Conversion of Lignocellulosic Corn Fiber to Butyrate - (Abstract Only) - (06-Mar-10)

Effect of compositional variability of Distillers' Grains on cellulosic ethanol production - (Peer Reviewed Journal)

Kim, Y., Hendrickson, R., Mosier, N.S., Ladisch, M.R., Bals, B., Balan, V., Dale, B.E., Dien, B.S., Cotta, M.A. 2010. Effect of compositional variability of Distillers' Grains on cellulosic ethanol production. Bioresource Technology. 101(14):5385-5393.

Optimizing On-farm Pretreatment of Perennial Grasses for Fuel Ethanol Production - (Peer Reviewed Journal)

Digman, M.F., Shinners, K.J., Casler, M.D., Dien, B.S., Hatfield, R.D., Jung, H.G., Muck, R.E., Weimer, P.J. 2010. Optimizing On-farm Pretreatment of Perennial Grasses for Fuel Ethanol Production. Bioresource Technology. 101:5305-5314.

Use of Coniochaeta ligniaria to Detoxify Fermentation Inhibitors Present in Cellulosic Sugar Streams - (Book / Chapter)

Nichols, N.N., Dien, B.S., Lopez, M.J., Moreno, J. 2010. Use of Coniochaeta ligniaria to Detoxify Fermentation Inhibitors Present in Cellulosic Sugar Streams. In: Hou, C.T., Shaw, J.-F., editors. Biocatalysis and Molecular Engineering. New York: John Wiley and Sons. p. 253-263.

Nutrient Recovery from the Dry Grind Process Using Sequential Micro and Ultrafiltration of Thin Stillage - (Peer Reviewed Journal)

Arora, A., Dien, B.S., Belyea, R.L., Singh, V., Tumbleson, M.E., Rausch, K.D. 2010. Nutrient Recovery from the Dry Grind Process Using Sequential Micro and Ultrafiltration of Thin Stillage. Bioresource Technology. 101(11):3859-3863.

Production of Butanol (A Biofuel) from Agricultural Residues: Part I - Use of Barley Straw Hydrolysate - (Peer Reviewed Journal)

Qureshi, N., Saha, B.C., Dien, B., Hector, R.E., Cotta, M.A. 2010. Production of Butanol (a Biofuel) from Agricultural Residues: Part I - Use of Barley Straw Hydrolysate. Biomass and Bioenergy. 34(4):559-565.

Production of Butanol (a Biofuel) from Agricultural Residues: Part II - Use of Corn Stover and Switchgrass Hydrolysates - (Peer Reviewed Journal)

Qureshi, N., Saha, B.C., Hector, R.E., Dien, B., Hughes, S., Liu, S., Iten, L., Bowman, M.J., Sarath, G., Cotta, M.A. 2010. Production of butanol (a Biofuel) from agricultural residues: Part II - Use of corn stover and switchgrass hydrolysates. Biomass and Bioenergy. 34(4):566-571.

Ethanol Production from SPORL-pretreated Lodgepole Pine: Preliminary Evaluation of Mass Balance and Process Energy Efficiency - (Peer Reviewed Journal)

Zhu, J.Y., Zhu, W., O Bryan, P.J., Dien, B.S., Tian, S., Gleisner, R., Pan, X.J. 2010. Ethanol Production from SPORL-Pretreated Lodgepole Pine: Preliminary Evaluation of Mass Balance and Process Energy Efficiency. Applied Microbiology and Biotechnology. 86(5):1355-1365.

Inhibition of cellulases by phenols - (Peer Reviewed Journal)

Ximenes, E., Kim, Y., Mosier, N., Dien, B.S., Ladisch, M. 2010. Inhibition of cellulases by phenols. Enzyme and Microbial Technology. 46(3-4):170-176.

Effects of forage quality and cell wall constituents of bermudagrass on biochemical conversion to ethanol - (Peer Reviewed Journal)

Anderson, W.F., Dien, B.S., Jung, H.G., Vogel, K.P., Weimer, P.J. 2010. Effects of forage quality and cell wall constituents of bermudagrass on biochemical conversion to ethanol. BioEnergy Research. 3:225-237.

Increasing Biomass Conversion Efficiencies to Ethanol by Engineering Energy Crops - (Proceedings)

Dien, B.S., Sarath, G., Pedersen, J.F., Funnell-Harris, D.L., Sattler, S.E., Cotta, M.A. 2009. Increasing Biomass Conversion Efficiencies to Ethanol by Engineering Energy Crops. Proceedings of the United States-Japan Natural Resources Food and Agriculture Panel. p. 37-40.

Anaerobic conversion of lignocellulosic corn fiber to butyric acid, a substrate for microbial butanol production - (Abstract Only) - (22-Jul-09)

Use of Coniochaeta ligniaria to detoxify fermentation inhibitors present in cellulosic sugar streams - (Abstract Only)

Nichols, N.N., Dien, B.S., Cotta, M.A. 2009. Use of Coniochaeta ligniaria to detoxify fermentation inhibitors present in cellulosic sugar streams [abstract]. American Society for Microbiology. Paper No. O-055.

Conversion of switchgrass to sugars and ethanol using dilute ammonium hydroxide pretreatment - (Abstract Only) - (06-May-09)

Evaluation of engineered xylose-fermenting industrial strains of Saccharomyces cerevisiae for improved ethanol production from lignocellulosic feedstocks - (Abstract Only) - (06-May-09)

Improved Sugar Conversion and Ethanol Yield for Forage Sorghum (Sorghum bicolor L. Moench) Lines with Reduced Lignin Contents - (Peer Reviewed Journal)

Dien, B.S., Sarath, G., Pedersen, J.F., Sattler, S.E., Chen, H., Funnell-Harris, D.L., Nichols, N.N., Cotta, M.A. 2009. Improved Sugar Conversion and Ethanol Yield for Forage Sorghum (Sorghum bicolor L. Moench) Lines with Reduced Lignin Contents. BioEnergy Research. 2(3):153-164.

Conversion of lower lignin mutants of Sorghum bicolor (L.) to ethanol - (Abstract Only) - (13-Mar-09)

Automated High Throughput GMAX-L Strains of Saccharomyces Cereviciae for Profitable Cellulosic Ethanol Production from Industrial Hydrosylates - (Abstract Only)

Hughes, S.R., Tasaki, K., Doll, K.M., Moser, B.R., Rich, J.O., Qureshi, N., Hector, R.E., Dien, B.S., Cotta, M.A., Bischoff, K.M., Liu, S., Bang, S., Jones, M. 2009. Automated high throughput GMAX-L strains of Saccharomyces cereviciae for profitable cellulosic ethanol production from industrial hydrosylates [abstract]. Society for In-Vitro Biology. p. 122.

Development of an Ethanol Yield Procedure for Dry-grind Corn Processing - (Peer Reviewed Journal)

Lemuz, C.R., Dien, B.S., Singh, V., Mckinney, J., Tumbleson, M.E., Rausch, K.D. 2009. Development of an Ethanol Yield Procedure for Dry-grind Corn Processing. Cereal Chemistry. 86(3):355-360.

Automated Yeast Transformation Protocol to Engineer S. cerevisiae Strains for Cellulosic Ethanol Production with Open Reading Frames that Express Proteins Binding to Xylose Isomerase Identified using Robotic Two-hybrid Screen - (Peer Reviewed Journal)

Hughes, S.R., Rich, J.O., Bischoff, K.M., Hector, R.E., Qureshi, N., Saha, B.C., Dien, B.S., Liu, S., Jackson Jr, J.S., Sterner, D.E., Butt, T.R., Labaer, J., Cotta, M.A. 2009. Automated yeast transformation protocol to engineer S. cerevisiae strains for cellulosic ethanol production with open reading frames that express proteins binding to xylose isomerase identified using robotic two-hybrid screen. Journal of the Association for Laboratory Automation. 8:200-212.

Automated production GMAX-L strains of Saccharomyces cereviciae for profitable sustainable cellulosic ethanol production combined with valuable coproduct isolation in mixed biorefinery settings - (Abstract Only)

Hughes, S.R., Doll, K., Moser, B., Bang, S., Rich, J., Qureshi, N., Hector, R., Dien, B., Cotta, M., Bischoff, K., Liu, S., Jones, M. 2009. Automated production GMAX-L strains of Saccharomyces cereviciae for profitable sustainable cellulosic ethanol production combined with valuable coproduct isolation in mixed biorefinery settings [abstract]. LabAutomation 2009. Abstract No. 109. p. 42.

Automated Yeast Mating Protocol Using Open Reading Frames from Saccharomyces cerevisiae Genome to Improve Yeast Strains for Cellulosic Ethanol Production - (Peer Reviewed Journal)

Hughes, S.R., Hector, R.E., Rich, J.O., Qureshi, N., Bischoff, K.M., Dien, B.S., Saha, B.C., Liu, S., Jackson Jr, J.S., Sterner, D.E., Butt, T.R., Labaer, J., Cotta, M.A. 2009. Automated yeast mating protocol using open reading frames from Saccharomyces cerevisiae genome to improve yeast strains for cellulosic ethanol production. Journal of the Association for Laboratory Automation. 8:190-199.

Mass Balances and Analytical Methods for Biomass Pretreatment Experiments - (Book / Chapter)

Dien, B.S. 2010. Mass Balances and Analytical Methods for Biomass Pretreatment Experiments. In: Vertes, A. A., Qureshi, N., Blaschek, H. P., Yukawa, H., editors. Biomass to Biofuels. Strategies for Global Industries. United Kingdom: Wiley and Sons. p. 213-231.

Agricultural Residues and Energy Crops as Novel Substrates for Butanol Production by Fermentation - (Abstract Only)

Qureshi, N., Saha, B.C., Iten, L.B., Sarath, G., Dien, B.S., Cotta, M.A. 2008. Agricultural Residues and Energy Crops as Novel Substrates for Butanol Production by Fermentation [abstract]. In: Proceedings of 10th International Workshop and Conference on Regulation of Metabolism, Genetics, and Development of Solvent and Acid Forming Clostridia, September 28-October 1, 2008, Holland, The Netherlands. Paper No. 19.

Simultaneous Saccharification and Fermentation and Partial Saccharification and Co-fermentation of Lignocellulosic Biomass for Ethanol Production - (Book / Chapter) - (26-Sep-08)

Engineered Saccharomyces cerevisiae strain for improved xylose utilization with a three-plasmid SUMO yeast expression system - (Peer Reviewed Journal)

Hughes, S.R., Sterner, D.E., Bischoff, K.M., Hector, R.E., Dowd, P.F., Qureshi, N., Bang, S.S., Grynavyski, N., Chakrabarty, T., Johnson, E.T., Dien, B.S., Mertens, J.A., Caughey, R.J., Liu, S., Butt, T.R., Labaer, J., Cotta, M.A., Rich, J.O. 2009. Engineered Saccharomyces cerevisiae strain for improved xylose utilization with a three-plasmid SUMO yeast expression system. Plasmid Journal. 61(1):22-38.

Challenges of converting agricultural abundance to biofuels: Agricultural Research Service-United States Department of Agriculture Research - (Abstract Only) - (28-Aug-08)

Opportunities for increasing biomass conversion efficiencies to ethanol by engineering energy crops - (Abstract Only) - (17-Aug-08)

Saccharomyces cerevisiae mass transformed with FLEXGenes results in strain capable of anaerobic fermentation of pentose and hexose sugars - (Abstract Only)

Hughes, S.R., Javers, J., Labaer, J., Butt, T., Hector, R.E., Dien, B.S., Saha, B.C., Bischoff, K.M., Kohl, S., Cotta, M.A., Rich, J.O. 2008. Saccharomyces cerevisiae mass transformed with FLEXGenes results in strain capable of anaerobic fermentation of pentose and hexose sugars [abstract]. Society of Industrial Microbiology. Session 34, S164, p. 106.

Thin Stillage Fractionation Using Ultrafiltration: Resistance in Series Model - (Peer Reviewed Journal)

Arora, A., Dien, B.S., Belyea, R.L., Wang, P., Singh, V., Tumbleson, M.E., Rausch, K.D. 2009. Thin Stillage Fractionation Using Ultrafiltration: Resistance in Series Model. Bioprocess and Biosystems Engineering. 32(2):225-233.

Saccharomyces cerevisiae engineered to convert pretreated lignocellulosic sugars anaerobically to ethanol - (Abstract Only)

Hughes, S.R., Bischoff, K.M., Hector, R.E., Dien, B.S., Saha, B.C., Javers, J., Gibbons, W., Bang, S., Nelson, R., Standish, K., Cotta, M.A., Rich, J.O. 2008. Saccharomyces cerevisiae engineered to convert pretreated Lignocellulosic sugars anaerobically to ethanol [abstract]. Cambridge Healthtech Institute, Second Generation Biofuels Development Summit, Seminar 6. p. 1.

Three-vector system for high-level functional expression of value-added co-products with xylose isomerase and xylulokinase in an industrial saccharomyces cerevisiae strain - (Abstract Only)

Hughes, S.R., Jackson Jr, J.S., Dowd, P.F., Hector, R.E., Bischoff, K.M., Sterner, D., Bang, S., Grynavyski, N., Chakrabarty, T., Johnson, E.T., Li, X., Caughey, R.J., Liu, S., Skory, C.D., Butt, T., Labaer, J., Dien, B.S., Saha, B.C., Bowen, M., Qureshi, N., Rich, J.O., Leathers, T.D., Cotta, M.A., Farrelly, P., Slininger P.J. 2008. Three-vector system for high-level functional expression of value-added co-products with Xylose Isomerase and Xylulokinase in an industrial Saccharomyces cerevisiae strain [abstract]. Cambridge Healthtech Institute, Second Generation Biofuels Development Summit. Poster #1. p. 4.

Evaluating low lignin mutants of forage sorghum for increased conversion efficiency to sugars and ethanol - (Abstract Only)

Dien, B.S., Sarath, G., Pedersen, J.F., Funnell-Harris, D.L., Sattler, S.E., Nichols, N.N. 2008. Evaluating low lignin mutants of forage sorghum for increased conversion efficiency to sugars and ethanol. Biotechnology for Fuels and Chemicals. p. 267.

Energy crops for ethanol: a processing perspective - (Abstract Only)

Dien, B.S. 2008. Energy crops for ethanol: a processing perspective. International Crop Science. Paper No. CS4-S4-06.

Energy crops for ethanol: a processing perspective - (Proceedings)

Dien, B.S., Sarath, G., Pedersen, J.F., Vogel, K.P., Jung, H.G., Sattler, S.E., Casler, M.D., Mitchell, R., Cotta, M.A. 2008. Energy crops for ethanol: a processing perspective. In: Proceedings of the 5th International Crops Science Congress, April 13-18, 2008, Jeju Island, Korea. p. 1-5.

Fungal metabolism of fermentation inhibitors present in corn stover dilute acid hydrolysate - (Peer Reviewed Journal)

Nichols, N.N., Sharma, L.N., Mowery, R.A., Chambliss, C.K., Van Walsum, G.P., Dien, B.S., Iten, L.B. 2008. Fungal metabolism of fermentation inhibitors present in corn stover dilute acid hydrolysate. Enzyme and Microbial Technology. 42(7):624-630.

PRODUCTION OF ETHANOL FROM CORN AND SUGARCANE - (Book / Chapter)

Nichols, N.N., Dien, B.S., Monceaux, D.A., Bothast, R.J. 2008. Production of ethanol from corn and sugarcane. In: Wall, J., Harwood, C.S., Demain, A.L., editors. Bioenergy. Chapter 1. Washington, DC: ASM Press. p. 3-15.

Process review of lignocellulose biochemical conversion to fuel ethanol - (Proceedings)

Dien, B.S. 2007. Process review of lignocellulose biochemical conversion to fuel ethanol. Proceedings of U.S. Frontiers of Engineering. p. 65-74.

Developing herbaceous energy crops as feedstocks for biofuel production - (Proceedings)

Dien, B.S., Jung, H.G., Vogel, K.P., Casler, M.D., Lamb, J.F., Iten, L.B., Mitchell, R., Sarath, G. 2007. Developing herbaceous energy crops as feedstocks for biofuel production. In: Proceedings of the UJNR Food and Agricultural Panel, October 21-25, 2007, Tsukuba, Japan. p. 137-140.

BUTANOL PRODUCTION BY CLOSTRIDIUM BEIJERINCKII. PART I. USE OF ACID AND ENZYME HYDROLYSED CORN FIBER - (Peer Reviewed Journal)

Qureshi, N., Ezeji, T.C., Ebener, J., Dien, B.S., Cotta, M.A., Blaschek, H.P. 2008. Butanol production by Clostridium beijerinckii. Part I. Use of acid and enzyme hydrolysed corn fiber. Bioresource Technology. 99:5915-5922.

Enzyme characterization for hydrolysis of AFEX and liquid hot-water pretreated distillers' grains and their conversion to ethanol - (Peer Reviewed Journal)

Dien, B.S., Ximenes, E.A., O'Bryan, P.J., Moniruzzaman, M., Li, X., Balan, V., Dale, B., Cotta, M.A. 2008. Enzyme characterization for hydrolysis of AFEX and liquid hot-water pretreated distillers' grains and their conversion to ethanol. Bioresource Technology. 99(12):5216-5225.

Composition of corn dry-grind ethanol by-products: DDGS, wet cake, and thin stillage - (Peer Reviewed Journal)

Kim, Y., Mosier, N.S., Hendrickson, R., Ezeji, T., Blaschek, H., Dien, B.S., Cotta, M.A., Dale, B., Ladisch, M.R. 2008. Composition of corn dry-grind ethanol by-products: DDGS, wet cake, and thin stillage. Bioresource Technology. 99(12):5165-5176.

Assessment of Bermudagrass and Bunch Grasses as Feedstock for Conversion to Ethanol - (Peer Reviewed Journal)

Anderson, W.F., Dien, B.S., Brandon, S.K., Peterson, J. 2008. Assessment of bermudagrass and bunch grasses as feedstock for conversion to ethanol. Applied Biochemistry and Biotechnology. 145(1-3):13-21.

FIBER SEPARATED FROM DISTILLERS DRIED GRAINS WITH SOLUBLES AS A FEEDSTOCK FOR ETHANOL PRODUCTION TO INCREASE OUTPUT FROM DRY GRIND CORN PLANTS - (Peer Reviewed Journal)

Srinivasan, R., Dien, B.S., Rausch, K.D., Tumbleson, M.E., Singh, V. 2007. Fiber Separated from Distillers Dried Grains with Solubles as a Feedstock for Ethanol Production. Cereal Chemistry. 84(6):563-566.

Coexpression of pyruvate decarboxylase and alcohol dehydrogenase genes in Lactobacillus brevis - (Peer Reviewed Journal)

Liu, S., Dien, B.S., Nichols, N.N., Bischoff, K.M., Hughes, S.R., Cotta, M.A. 2007. Coexpression of pyruvate decarboxylase and alcohol dehydrogenase genes in Lactobacillus brevis. FEMS Microbiolology Letters. 274(2):291-297.

A PRIMER FOR LIGNOCELLULOSE BIOCHEMICAL CONVERSION TO FUEL ETHANOL - (Proceedings)

Dien, B.S. 2007. A primer for lignocellulose biochemical conversion to fuel ethanol. Proceedings of the Fifth International Starch Technology Conference. p. 5.1-5.9.

Monensin inhibits growth of bacterial contaminants from fuel ethanol plants - (Abstract Only)

Bischoff, K.M., Liu, S., Dien, B.S. 2007. Monensin inhibits growth of bacterial contaminants from fuel ethanol plants [abstract]. American Society for Microbiology. Poster #0-010. p. 60.

EVALUATING HERBACEOUS PERENNIALS AS ENERGY CROPS FOR PRODUCTION OF FUEL ETHANOL - (Abstract Only) - (02-May-07)

A NOVEL PRETREATMENT (DIETHYL OXALATE) RELEASING FERMENTABLE SUGARS AND USEFUL FIBERS FROM LIGNOCELLULOSE - (Abstract Only) - (02-May-07)

REMOVAL OF INHIBITORS FROM BIOMASS SUGARS USING A BIOLOGICAL PROCESS - (Abstract Only) - (02-May-07)

Assessment of Bermudagrass as a Feedstock for Ethanol - (Abstract Only)

Anderson, W.F., Dien, B.S., Peterson, J., Brandon, S.K., Akin, D.E. 2007. Assessment of bermudagrass as a feedstock for ethanol. [abstract]. ASA-CSSA-SSSA International Meetings, November 4-8, 2007, New Orleans, Louisiana. Cd Rom.

DEVELOPING HERBACEOUS ENERGY CROPS AS FEEDSTOCKS FOR BIOETHANOL PRODUCTION - (Abstract Only)

Dien, B.S., Cotta, M.A., Jung, H.G., Vogel, K.P. 2007. Developing herbaceous energy crops as feedstocks for bioethanol production [abstract]. American Chemical Society. Paper No. Agro-148.

BIOLOGICAL ABATEMENT FOR REMOVAL OF INHIBITORS FROM BIOMASS SUGARS - (Abstract Only) - (29-Mar-07)

Issues in the production and conversion of lignocellulosic biomass crops to ethanol - (Abstract Only)

Jung, H.G., Dien, B.S., Vogel, K.P. 2007. Issues in the production and conversion of lignocellulosic biomass crops to ethanol [abstract]. 233rd American Chemical Society National Meeting & Exposition, March 25-29, 2007, Chicago, IL. p. 107.

PRODUCTION OF HIGHLY ACTIVE LIGNOCELLULOSE-DEGRADING ENZYMES OF ANAEROBIC FUNGI BY INDUSTRIALLY RELEVANT FUNGI - (Abstract Only)

Li, X., Skory, C.D., Ximenes, E.A., Jordan, D.B., Dien, B.S., Hughes, S.R., Cotta, M.A. 2007. Production of highly active lignocellulose-degrading enzymes of anaerobic fungi by industrially relevant fungi [abstract]. Physiology to Genomics to Fuels.

ON-FARM PRETREATMENT TECHNOLOGIES FOR IMPROVING ENZYMATIC DIGESTIBILITY OF CELLULOSE AND HEMICELLULOSE PRESENT IN PERENNIAL GRASS AND CORN STOVER - (Abstract Only)

Digman, M.F., Shinners, K.J., Dien, B.S., Muck, R.E., Weimer, P.J. 2007. On-farm pretreatment technologies for improving enzymatic digestibility of cellulose and hemicellulose present in perennial grass and corn stover [abstract]. Abstracts of the 29th Symposium on Biotechnology for Fuels and Chemicals, April 29-May 2, 2007, Denver, Colorado. p. 147.

ON-FARM PRETREATMENT TECHNOLOGIES FOR IMPROVING ENZYMATIC DIGESTIBILITY OF CELLULOSE AND HEMICELLULOSE PRESENT IN PERENNIAL GRASS - (Proceedings)

Digman, M.F., Shinners, K.J., Dien, B.S., Hatfield, R.D., Li, X., Muck, R.E., Weimer, P.J. 2007. On-farm pretreatment technologies for improving enzymatic digestibility of cellulose and hemicellulose present in perennial grass. In: Proceedings of 2007 Annual Meeting of American Society of Agricultural and Biological Engineers, June 17, 2007, Minneapolis, Minnesota. Paper No. 071021.

A primer for lignocellulose biochemical conversion to fuel ethanol - (Book / Chapter)

Dien, B.S., Bothast, R.J. 2009. A primer for lignocellulose biochemical conversion to fuel ethanol. In: Ingledew,W.M., Austin, G.D., Kluhspies, C., Kelsall, D.R., editors. The Alcohol Textbook. Nottingham, U.K.: Nottingham University Press. p. 73-94.

Genotypic Evaluation of Bermudagrass for Conversion to Ethanol - (Abstract Only)

Anderson, W.F., Dien, B.S., Peterson, J., Akin, D.E. 2007. Genotypic Evaluation of Bermudagrass for Conversion to Ethanol. Proceedings: 29th Symposium on Biotechnology for Fuels and Chemicals, Denver, CO, April 29-May 2, 2007.

Issues in the production and conversion of lignocellulosic biomass crops to ethanol - (Proceedings)

Jung, H.G., Dien, B.S., Vogel, K.P. 2007. Issues in the production and conversion of lignocellulosic biomass crops to ethanol. 233rd American Chemical Society National Meeting, March 25-29, 2007, Chicago, Illinois. Available: http://oasys2.confex.com/acs/233nm/techprogram/P1061547.htm.

Issues in the production and conversion of lignocellulosic biomass crops to ethanol - (Proceedings)

Jung, H.G., Dien, B.S., Vogel, K.P. 2007. Issues in the production and conversion of lignocellulosic biomass crops to ethanol. In: 38th Northwest Alfalfa Seed Growers Association Winter Seed Conference Proceedings, January 22-23, 2007, Las Vegas, Nevada. p. 17-19.

Issues in the production and conversion of lignocellulosic biomass crops to ethanol - (Proceedings)

Jung, H.G., Dien, B.S., Vogel, K.P. 2007. Issues in the production and conversion of lignocellulosic biomass crops to ethanol. In: Midwest Forage Association Symposium and Annual Meeting Proceedings, January 30-31, 2007, Wisconsin Dells, Wisconsin. CD-ROM.

FORAGE ENERGY CROPS AS FEEDSTOCKS FOR PRODUCTION OF FUEL ETHANOL - (Abstract Only)

Cotta, M.A., Dien, B.S., Iten, L.B., Jung, H.G., Lamb, J.F., Vogel, K.P., Mitchell, R., Sarath, G., Casler, M.D., Weimer, P.J. 2006. Forage energy crops as feedstocks for production of fuel ethanol [abstract]. Biocatalysis and Bioenergy. p. 21.

EXPRESSION OF AN AT-RICH XYLANASE GENE FROM THE ANAEROBIC FUNGUS ORPINOMYCES SP. STRAIN PC-2 IN AND SECRETION OF THE HETEROLOGOUS ENZYME BY HYPOCREA JECORINA - (Peer Reviewed Journal)

Li, X., Skory, C.D., Ximenes, E.A., Jordan, D.B., Dien, B.S., Hughes, S.R., Cotta, M.A. 2007. Expression of an AT-rich xylanase gene from the anaerobic fungus Orpinomyces sp. strain PC-2 in and secretion of the heterologous enzyme by Hypocrea jecorina. Applied Microbiology and Biotechnology. 74:1264-1275.

ENZYME PRODUCTION BY INDUSTRIALLY RELEVANT FUNGI CULTURED ON CO-PRODUCT FROM CORN DRY GRIND ETHANOL PLANTS - (Peer Reviewed Journal)

Ximenes, E., Dien, B.S., Ladisch, M.R., Mosier, N., Cotta, M.A., Li, X. 2007. Enzyme production by industrially relevant fungi cultured on coproduct from corn dry grind ethanol plants. Applied Biochemistry and Biotechnology. 136-140:171-183.

HEMICELLULASES FOR MEDIATING BIOMASS SACCHARIFICATION - (Proceedings)

Jordan, D.B., Dien, B.S., Li, X., Cotta, M.A. 2006. Hemicellulases for mediating biomass saccharification. In: Proceedings of Renewable Energy 2006, October 9-13, 2006, Makuhari Messe, Chiba, Japan. p. 1036-1041.

HEMICELLULASES FOR MEDIATING BIOMASS SACCHARIFICATION - (Abstract Only) - (13-Oct-06)

REVIEW OF CURRENT RESEARCH DIRECTIONS FOR PRODUCING BIOLOGICAL ETHANOL AT THE NATIONAL CENTER FOR AGRICULTURAL UTILIZATION RESEARCH - (Abstract Only)

Dien, B.S., Saha, B.C. 2006. Review of current research directions for producing biological ethanol at the National Center for Agricultural Utilization Research [abstract]. Advancing Renewable Energy. p. 27.

CULTURE CONTAINING BIOMASS ACID HYDROLYSATE AND CONIOCHAETA LIGNIARIA FUNGUS - (Patent Application)

Nichols, N.N., Lopez, M.J., Dien, B.S., Bothast, R.J. 2006. Culture containing biomass acid hydrolysate and Coniochaeta ligniaria fungus. U.S. Patent 7,067,303.

LIGNOCELLULOSE-DEGRADING ENZYMES PRODUCED BY THE ASCOMYCETE CONIOCHAETA LIGNIARIA AND RELATED SPECIES: APPLICATION FOR A LIGNOCELLULOSIC SUBSTRATE TREATMENT - (Peer Reviewed Journal)

Lopez, M.J., Vargas-Garcia, M., Suarez-Estrella, F., Nichols, N.N., Dien, B.S., Moreno, J. 2007. Lignocellulose-degrading enzymes produced by the ascomycete Coniochaeta ligniaria and related species: application for a lignocellulosic substrate treatment. Enzyme and Microbial Technology. 40:794-800.

PRETREATMENT AND HYDROLYSIS OF DISTILLER'S GRAINS TO FERMENTABLE SUGARS: AN INTEGRATED APPROACH BY THE MIDWEST CONSORTIUM FOR SUSTAINABLE BIOBASED PRODUCTS AND ENERGY - (Abstract Only)

Cotta, M.A., Dien, B.S., Li, X., Ladisch, M., Mosier, N., Tyner, W., Woodson, R., Blaschek, H., Dale, B., Shanks, B., Verkade, J. 2006. Pretreatment and hydrolysis of distiller's grains to fermentable sugars: an integrated approach by the Midwest Consortium for sustainable biobased products and energy [abstract]. Corn Utilization. Paper No. 24.

IDENTIFICATION AND TRANSCRIPTIONAL PROFILING OF FUROIC ACID METABOLISM GENES IN PSEUDOMONAS PUTIDA - (Abstract Only)

Nichols, N.N., Mertens, J.A., Dien, B.S. 2006. Identification and transcriptional profiling of furoic acid metabolism genes in Pseudomonas putida [abstract]. American Society for Microbiology. Paper No. Q-449.

AN OVERVIEW OF RECENT ADVANCEMENTS IN LIGNOCELLULOSE TO ETHANOL CONVERSION TECHNOLOGY - (Abstract Only)

Dien, B.S., Nichols, N.N., Li, X., Cotta, M.A. 2006. An overview of recent advancements in lignocellulose to ethanol conversion technology [abstract]. International Conference on Bioenergy. p. 8.

ENZYME PRODUCTION BY INDUSTRIALLY RELEVANT FUNGI GROWING ON FEED CO-PRODUCT FROM DRY MILL ETHANOL PLANTS - (Abstract Only)

Ximenes, E.A., Dien, B.S., Ladisch, M.R., Mosier, N., Cotta, M.A., Li, X. 2006. Enzyme production by industrially relevant fungi growing on feed co-product from dry mill ethanol plants [abstract]. Biotechnology for Fuels and Chemicals. p. 107.

RESEARCH CHALLENGES AND OPPORTUNITIES FOR CELLULOSE CONVERSION TECHNOLOGY IN A DRY MILL PATHWAY - (Abstract Only)

Ladisch, M., Tyner, W., Mosier, N., Cotta, M.A., Dien, B.S., Blaschek, H., Dale, B., Shanks, B., Petersen, G. 2006. Research challenges and opportunities for cellulose conversion technology in a dry mill pathway [abstract]. Biotechnology for Fuels and Chemicals. p. 48.

ON-FARM PRETREATMENT OF BIOMASS: IMPACT OF GENETICS AND MANAGEMENT - (Abstract Only) - (08-Apr-06)

CODON OPTIMIZATION OF AN A+T RICH XYLANASE GENE FROM THE ANAEROBIC FUNGUS ORPINOMYCES PC-2 FOR EXPRESSION BY TRICHODERMA REESEI - (Abstract Only)

Li, X., Jordan, D.B., Ximenes, E.A., Cotta, M.A., Dien, B.S., Hughes, S.R. 2006. Codon optimization of an A+T rich xylanase gene from the anaerobic fungus Orpinomyces PC-2 for expression by Trichoderma reesei [abstract]. In: Proceedings of the 9th International Workshop on Trichoderma and Gliocladium, April 6-8, 2006, Vienna, Austria. Paper No. T3.

NITROGEN SOURCE AND MINERAL OPTIMIZATION ENHANCE D-XYLOSE CONVERSION TO ETHANOL BY THE YEAST PICHIA STIPITIS NRRL Y-7124 - (Peer Reviewed Journal)

Slininger, P.J., Dien, B.S., Gorsich, S.W., Liu, Z. 2006. Nitrogen source and mineral optimization enhance D-xylose conversion to ethanol by the yeast Pichia stipitis NRRL Y-7124. Applied Microbiology and Biotechnology. 72(6):1285-1296.

DEVELOPMENT OF FORAGE CROPS AS FEEDSTOCKS FOR PRODUCTION OF FUEL ETHANOL - (Abstract Only)

Cotta, M.A., Dien, B.S., Jung, H.G., Vogel, K.P., Casler, M.D., Lamb, J.F., Weimer, P.J., Iten, L.B., Mitchell, R., Sarath, G. 2006. Development of forage crops as feedstocks for production of fuel ethanol [abstract]. International Conference on Bioenergy. p. I-5.

CHEMICAL COMPOSITION AND RESPONSE TO DILUTE-ACID PRETREATMENT AND ENZYMATIC SACCHARIFICATION OF ALFALFA, REED CANARYGRASS, AND SWITCHGRASS - (Peer Reviewed Journal)

Dien, B.S., Jung, H.G., Vogel, K.P., Casler, M.D., Lamb, J.F., Iten, L.B., Mitchell, R., Sarath, G. 2006. Chemical composition and response to dilute-acid pretreatment and enzymatic saccharification of alfalfa, reed canarygrass, and switchgrass. Biomass and Bioenergy. 30:880-891.

ENZYMATIC SACCHARIFICATION OF HOT-WATER PRETREATED CORN FIBER FOR PRODUCTION OF MONOSACCHARIDES - (Peer Reviewed Journal)

Dien, B.S., Li, X., Iten, L.B., Jordan, D.B., Nichols, N.N., O Bryan, P.J., Cotta, M.A. 2006. Enzymatic saccharification of hot-water pretreated corn fiber for production of monosaccharides. Enzyme and Microbial Technology. 39:1137-1144.

CONTINUOUS PRODUCTION OF ETHANOL IN HIGH PRODUCTIVITY BIOREACTORS USING GENETICALLY ENGINEERED ESCHERICHIA COLI FBR5: MEMBRANE AND FIXED CELL REACTORS - (Abstract Only)

Qureshi, N., Dien, B.S., Nichols, N.N., Liu, S., Hughes, S.R., Iten, L.B., Saha, B.C., Cotta, M.A. 2005. Continuous production of ethanol in high productivity bioreactors using genetically engineered Escherichia coli FBR5: membrane and fixed cell reactors [extended abstract]. American Institute of Chemical Engineers. Paper No. 589g.

CONTINUOUS PRODUCTION OF ETHANOL IN HIGH PRODUCTIVITY BIOREACTORS USING ESCHERICHIA COLI FBR5: MEMBRANE AND FIXED CELL REACTORS - (Abstract Only)

Qureshi, N., Dien, B.S., Nichols, N.N., Liu, S., Iten, L.B., Saha, B.C., Cotta, M.A. 2005. Continuous production of ethanol in high productivity bioreactors using Escherichia coli FBR5: membrane and fixed cell reactors [abstract]. American Institute of Chemical Engineers. Paper No. 589g.

LACTOBACILLUS BREVIS: A POTENTIAL BIOCATALYST FOR LIGNOCELLULOSIC BIOMASS TO ETHANOL - (Abstract Only)

Liu, S., Dien, B.S., Cotta, M.A., Bischoff, K.M., Hughes, S.R. 2005. Lactobacillus brevis: a potential biocatalyst for lignocellulosic biomass to ethanol [abstract]. Society of Industrial Microbiology. Paper #P05.

ETHANOL FERMENTATION OF SUGARS IN CORN STOVER DILUTE ACID HYDROLYSATES - (Abstract Only)

Nichols, N.N., Dien, B.S., Cotta, M.A. 2005. Ethanol fermentation of sugars in corn stover dilute acid hydrolysates [abstract]. Society of Industrial Microbiology. p. 85.

MINERAL AND NITROGEN SOURCE OPTIMIZATION ENHANCE D-XYLOSE CONVERSION TO ETHANOL BY THE YEAST PICHIA STIPITIS - (Abstract Only)

Slininger, P.J., Dien, B.S., Gorsich, S.W., Liu, Z. 2005. Mineral and nitrogen source optimization enhance d-xylose conversion to ethanol by the yeast Pichia stipitis [abstract]. Society of Industrial Microbiology Annual Meeting. Paper No. P07.

GENETICALLY ENGINEERED ESCHERICHIA COLI FOR ETHANOL PRODUCTION FROM XYLOSE: SUBSTRATE AND PRODUCT INHIBITION AND KINETIC PARAMETERS - (Peer Reviewed Journal)

Qureshi, N., Dien, B.S., Nichols, N.N., Saha, B.C., Cotta, M.A. 2006. Genetically engineered Escherichia coli for ethanol production from xylose: substrate and product inhibition and kinetic parameters. Institution of Chemical Engineers Transactions. 84(2):114-122.

TOLERANCE TO FURFURAL-INDUCED STRESS IS ASSOCIATED WITH PENTOSE PHOSPHATE PATHWAY GENES ZWF1, GND1, RPE1,AND TKL1 IN SACCHAROMYCES CEREVISIAE - (Peer Reviewed Journal)

Gorsich, S.W., Dien, B.S., Nichols, N.N., Slininger, P.J., Liu, Z., Skory, C.D. 2005. Tolerance to furfural-induced stress is associated with pentose phosphate pathway genes ZWF1, GND1, RPEL, and TKL1 in Saccharomyces cerevisiae. Applied Microbiology and Biotechnology. 71(3):339-349. DOI: 10.1007/s00253-005-0142-3

ENZYMATIC SACCHARIFICATION OF PRETREATED CORN FIBER FOR PRODUCTION OF SUGARS - (Abstract Only)

Dien, B.S., Li, X., Jordan, D.B., Nichols, N.N., Iten, L.B., Cotta, M.A. 2005. Enzymatic saccharification of pretreated corn fiber for production of sugars [abstract]. International Starch Technology. p. 90.

USE OF FIELD PEA STARCH AS A FEEDSTOCK FOR ETHANOL FERMENTATION - (Abstract Only)

Nichols, N.N., Dien, B.S., Wu, V., Cotta, M.A. 2005. Use of field pea starch as a feedstock for ethanol fermentation [abstract]. International Starch Technology. p. 96.

THE CORN ETHANOL INDUSTRY - (Book / Chapter)

Nichols, N.N., Dien, B.S., Bothast, R.J., Cotta, M.A. 2006. The corn ethanol industry. In: Minteer, S., editor. Alcoholic Fuels. Boca Raton, FL: CRS Press. p. 59-78.

METABOLIC ENGINEERING OF A LACTOBACILLUS PLANTARUM DOUBLE LDH KNOCKOUT STRAIN FOR ENHANCED ETHANOL PRODUCTION - (Peer Reviewed Journal)

Liu, S., Nichols, N.N., Dien, B.S., Cotta, M.A. 2006. Metabolic engineering of a Lactobacillus plantarum double LDH knockout strain for enhanced ethanol production. Journal of Industrial Microbiology and Biotechnology. 33(1):1-7.

HYDROLYSIS AND FERMENTATION OF PERICARP AND ENDOSPERM FIBER RECOVERED FROM ENZYMATIC CORN DRY GRIND PROCESS - (Peer Reviewed Journal)

Dien, B.S., Johnston, D., Hicks, K.B., Cotta, M.A., Singh, V. 2005. Hydrolysis and fermentation of pericarp and endosperm fibers recovered from enzymatic corn dry-grind process. Cereal Chemistry. 82(5):616-620.

ETHANOL FERMENTATION OF STARCH FROM FIELD PEAS - (Peer Reviewed Journal)

Nichols, N.N., Dien, B.S., Wu, Y., Cotta, M.A. 2005. Ethanol fermentation of starch from field peas. Cereal Chemistry. 82(5):554-558.

ENZYMATIC SACCHARIFICATION OF PRETREATED CORN FIBER FOR PRODUCTION OF SUGARS - (Abstract Only)

Dien, B.S., Li, X., Cotta, M.A. 2005. Enzymatic saccharification of pretreated corn fiber for production of sugars [abstract]. Biotechnology for Fuels and Chemicals. Paper No. B-36.

ETHANOL FERMENTATION BY OVEREXPRESSING A GRAM-POSITIVE PDC GENE IN LACTOBACILLUS PLANTARUM STRAIN TF103 - (Abstract Only)

Liu, S., Nichols, N.N., Dien, B.S., Cotta, M.A. 2005. Ethanol fermentation by overexpressing a Gram-positive PDC gene in Lactobacillus plantarum strain TF103 [abstract]. Biotechnology for Fuels and Chemicals Symposium Proceedings, May 1-4, 2005, Denver, Colorado. p. 158.

METABOLIC ENGINEERING OF LACTOBACILLUS BREVIS FOR ETHANOL PRODUCTION - (Abstract Only)

Liu, S., Dien, B.S. 2005. Metabolic engineering of Lactobacillus brevis for ethanol production [abstract]. The World Congress on Industrial Biotechnology and Bioprocessing. p. 23.

FUNCTIONAL EXPRESSION OF BACTERIAL ZYMOBACTER PALMAE PYRUVATE DECARBOXYLASE GENE IN LACTIC ACID BACTERIA - (Peer Reviewed Journal)

Liu, S., Dien, B.S., Cotta, M.A. 2005. Functional expression of bacterial Zymobacter palmae pyruvate decarboxylase gene in lactic acid bacteria. Current Microbiology. 50:1-6.

REVIEW OF PROCESS FOR PRODUCING CORN FIBER OIL AND ETHANOL FROM "QUICK FIBER" - (Review Article)

Dien, B.S., Nagle, N., Singh, V., Moreau, R.A., Tucker, M.P., Nichols, N.N., Johnston, D., Cotta, M.A., Hicks, K.B., Nguyen, Q., Bothast, R.J. 2005. Review of process for producing corn fiber oil and ethanol from "Quick Fiber." International Sugar Journal. 107(1275):187-191.

PROFILE OF ENZYME PRODUCTION OF TRICHODERMA REESEI GROWN ON CORN FIBER FRACTIONS - (Peer Reviewed Journal)

Li, X., Dien, B.S., Cotta, M.A., Wu, Y., Saha, B.C. 2005. Profile of enzyme production of Trichoderma reesei grown on corn fiber fractions. Applied Biochemistry and Biotechnology. 121-124:321-334.

CONVERTING HERBACEOUS ENERGY CROPS TO BIOETHANOL: A REVIEW WITH EMPHASIS ON PRETREATMENT PROCESSES - (Book / Chapter)

Dien, B.S., Iten, L.B., Skory, C.D. 2005. Converting herbaceous energy crops to bioethanol: a review with emphasis on pretreatment processes. In: Hou, C.T., editor. Handbook of Industrial Biocatalysis. Chapter 23. Boca Raton, FL: Taylor & Francis Group. p. 1-11.

BIOABATEMENT TO REMOVE INHIBITORS FROM BIOMASS-DERIVED SUGAR HYDROLYSATES - (Peer Reviewed Journal)

Nichols, N.N., Dien, B.S., Guisado, G.M., Lopez, M.J. 2005. Bioabatement to remove inhibitors from biomass-derived sugar hydrolysates. Applied Biochemistry and Biotechnology. 121-124:379-390.

INDUSTRIAL SCALE-UP OF PH CONTROLLED LIQUID HOT WATER PRETREATMENT OF CORN FIBER FOR FUEL ETHANOL PRODUCTION - (Peer Reviewed Journal)

Mosier, N.S., Hendrickson, R., Brewer, M., Ho, N., Sedlak, M., Dreshel, R., Welch, G., Dien, B.S., Aden, A., Ladisch, M.R. 2005. Industrial scale-up of ph-controlled liquid hot water pretreatment of corn fiber for fuel ethanol production. Applied Biochemistry and Biotechnology. 125:77-97.

IN VITRO GAS PRODUCTION AS A SURROGATE MEASUREMENT OF THE FERMENTABILITY OF CELLULOSIC BIOMASS - (Peer Reviewed Journal)

Weimer, P.J., Dien, B.S., Springer, T.L., Vogel, K.P. 2005. In vitro gas production as a surrogate measurement of the fermentability of cellulosic biomass. Applied Microbiology Biotechnology. 67:52-58.

RECOMBINANT BIOCATALYSTS FOR CONVERTING SUGAR MIXTURES TO LACTIC ACID - (Abstract Only)

Dien, B.S., Whitehead, T.R., Nichols, N.N., Skory, C.D., Cotta, M.A. 2004. Recombinant biocatalysts for converting sugar mixtures to lactic acid [abstract]. Great Lakes Regional American Chemical Society Symposium. Paper No. 40.

A BIOLOGICAL APPROACH TO REMOVING INHIBITORY COMPOUNDS FROM BIOMASS SUGARS TO BE USED FOR FERMENTATION. - (Abstract Only)

Nichols, N.N., Dien, B.S., Lopez, M.J. 2004. A biological approach to removing inhibitory compounds from biomass sugars to be used for fermentation [abstract]. Great Lakes Regional American Chemical Society Symposium. Paper No. 36.

ADVANCES ON ENZYMATIC SACCHARIFICATION OF LIGNOCELLULOSIC BIOMASS - (Abstract Only)

Li, X., Jordan, D.B., Dien, B.S., Cotta, M.A., Kane, P.M. 2004. Advances on enzymatic saccharification of lignocellulosic biomass [abstract]. Great Lakes Regional American Chemical Society. Paper No. 04-035.

ADSORBED CELL DYNAMIC BIOFILM REACTOR FOR ETHANOL PRODUCTION FROM XYLOSE AND CORN FIBER HYDROLYSATE - (Abstract Only)

Qureshi, N., Brining, H.R., Iten, L.B., Dien, B.S., Nichols, N.N., Saha, B.C., Cotta, M.A. 2004. Adsorbed cell dynamic biofilm reactor for ethanol production from xylose and corn fiber hydrolysate [abstract]. Great Lakes Regional American Chemical Society Symposium. p. 179.

THE SACCHAROMYCES CEREVISIAE PENTOSE PHOSPHATE PATHWAY GENE, RPE1, FUNCTIONS IN FURFURAL TOLERANCE DURING FERMENTATION - (Proceedings)

Gorsich, S.W., Dien, B.S., Nichols, N.N., Slininger, P.J., Liu, Z. 2004. The Saccharomyces cerevisiae pentose phosphate pathway gene, rpe1, functions in furfural tolerance during fermentation [abstract]. Proceedings of the 11th International Congress on Yeasts in Science and Biotechnology. Paper No. PM24.

A COMPARISON BETWEEN CONVERSION OF PERICARP AND ENDOSPERM FIBER FROM CORN INTO ETHANOL - (Proceedings)

Singh, V., Dien, B.S., Johnston, D., Hicks, K.B., Cotta, M.A. 2004. A comparison between conversion of pericarp and endosperm fiber from corn into ethanol. Proceedings of ASAE Annual International Meeting. p. 1-15.

IDENTIFICATION OF SACCHAROMYCES CEREVISIAE GENES INVOLVED IN FURFURAL TOLERANCE DURING FERMENTATION. - (Abstract Only)

Gorsich, S.W., Slininger, P.J., Liu, Z., Nichols, N.N., Dien, B.S. 2004. Identification of Saccharomyces cerevisiae genes involved in furfural tolerance during fermentation [abstract]. Proceedings of the Yeast Genetics and Molecular Biology. Abstract No. 166A, p. 104.

A STANDARDIZED LABORATORY PROCEDURE FOR ETHANOL YIELD MEASUREMENT - (Abstract Only)

Tumbleson, M., Lemuz, C., Dien, B.S., Singh, V., Belyea, R., Rausch, K. 2004. A standardized laboratory procedure for ethanol yield measurement [abstract]. International Fuel Ethanol Workshop. Paper No. 61.

ETHANOL YIELD DETERMINATION FOR DRY GRIND CORN PROCESSING - (Proceedings)

Lemuz, C.R., Dien, B.S., Tumbleson, M.E., Singh, V., Rausch, K.D. 2004. Ethanol yield determination for dry grind corn processing. Proceedings Corn Utilization and Technology Conference. p. 61.

EXPRESSION ANALYSIS OF PYRUVATE DECARBOXYLASE GENES FROM ZYMOBACTER PALMAE AND SARCINA VENTRICULI IN GRAM-POSITIVE LACTIC ACID BACTERIA - (Abstract Only)

Liu, S., Nichols, N.N., Dien, B.S., Cotta, M.A. 2004. Expression analysis of pyruvate decarboxylase genes from Zymobacter palmae and Sarcina ventriculi in Gram-positive lactic acid bacteria [abstract]. American Society for Microbiology. Paper No. O-099.

ENGINEERING KLEBSIELLA OXYTOCA FOR PRODUCTION OF LACTIC ACID FROM HEXOSE AND PENTOSE - (Abstract Only)

Dien, B.S., Whitehead, T.R., Nichols, N.N., Cotta, M.A. 2004. Engineering klebsiella oxytoca for production of lactic acid from hexose and pentose [abstract]. Biotechnology for Fuels and Chemicals. Paper No. 2-08.

USE OF THE ASCOMYCETE CONIOCHAETA LIGNIARIA TO REMOVE INHIBITORS FROM BIOMASS-DERIVED SUGARS - (Abstract Only)

Nichols, N.N., Dien, B.S., Guisado, G.M., Lopez, M.J. 2004. Use of the ascomycete coniochaeta ligniaria to remove inhibitors from biomass-derived sugars [abstract]. Biotechnology For Fuels And Chemicals Symposium. Paper No. 2-10.

GROWTH OF AND ENZYME PRODUCTION BY TRICHODERMA REESEI ON CORN FIBER FRACTIONS - (Abstract Only)

Li, X., Dien, B.S., Cotta, M.A. 2004. Growth of and enzyme production by Trichoderma reesei on corn fiber fractions [abstract]. Biotechnology For Fuels And Chemicals. p. 25.

GENETICALLY ENGINEERED ESCHERICHIA COLI FOR ETHANOL PRODUCTION FROM PENTOSE SUGARS: SUBSTRATE AND PRODUCT INHIBITION AND KINETIC PARAMETERS - (Abstract Only)

Qureshi, N., Dien, B.S., Nichols, N.N., Cotta, M.A. 2004. Genetically engineered Escherichia coli for ethanol production from pentose sugars: substrate and product inhibition and kinetic parameters [abstract]. Biotechnology for Fuels and Chemicals. Paper No. 5-23.

ADAPTIVE RESPONSE OF YEASTS TO FURFURAL AND 5-HYDROXYMETHYLFURFURAL AND NEW CHEMICAL EVIDENCE FOR HMF CONVERSION TO 2,5-BIS-HYDROXYMETHYLFURAN - (Peer Reviewed Journal)

Liu, Z., Slininger, P.J., Dien, B.S., Berhow, M.A., Kurtzman, C.P., Gorsich, S.W. 2004. Adaptive response of yeasts to furfural and 5-hydroxymethylfurfural and new chemical evidence for hmf conversion to 2,5-bis-hydroxymethylfuran. Journal of Industrial Microbiology and Biotechnology. 31:345-352. Available http://dx.doi.org/10.1007/s10295-004-0148-3.

FERMENTATION OF HEXOSE AND PENTOSE SUGAR MIXTURES TO LACTIC ACID BY RECOMBINANT BACTERIA - (Abstract Only)

Dien, B.S., Nichols, N.N., Cotta, M.A. 2003. Fermentation of hexose and pentose sugar mixtures to lactic acid by recombinant bacteria [abstract]. American Institute Of Chemical Engineers. Paper No. 161D.

OPTIMIZATION OF CONTROLLED PH LIQUID HOT WATER PRETREATMENT OF CORN FIBER AND STOVER - (Abstract Only)

Mosier, N.S., Kim, Y., Zeng, M., Hendrickson, R., Dien, B.S., Welsh, G., Ladisch, M. 2003. Optimization of controlled pH liquid hot water pretreatment of corn fiber and stover [abstract]. American Institute Of Chemical Engineers. Paper No. 163d.

DEVELOPMENT OF MICROBIAL BIOCATALYSTS TO PRODUCE FUELS FROM AGRICULTURAL BIOMASS - (Proceedings)

Cotta, M.A., Nichols, N.N., Dien, B.S., Li, X. 2003. Development of microbial biocatalysts to produce fuels from agricultural biomass. Proceedings 32nd annual United States Japan Natural Resources Protein Panel. p. 387-393.

PROPERTIES OF A RECOMBINANT BETA-GLUCOSIDASE FROM THE POLYCENTRIC ANAEROBIC FUNGUS ORPINOMYCES PC-2 AND ITS APPLICATION FOR CELLULOSE HYDROLYSIS - (Peer Reviewed Journal)

Li, X., Ljungdahl, L.G., Ximenes, E.A., Chen, H., Felix, C.R., Cotta, M.A., Dien, B.S. 2004. Properties of a recombinant beta-glucosidase from the polycentric anaerobic fungus Orpinomyces PC-2 and its application for cellulose hydrolysis. Journal of Applied Biochemistry and Biotechnology. 113:233-250.

FERMENTATION OF "QUICK FIBER" PRODUCED FROM A MODIFIED CORN MILLING PROCESS INTO ETHANOL AND RECOVERY OF CORN FIBER OIL - (Peer Reviewed Journal)

Dien, B.S., Nagle, N., Singh, V., Moreau, R.A., Nichols, N.N., Johnston, D., Cotta, M.A., Hicks, K.B., Nguyen, Q., Bothast, R.J. 2004. Fermentation of "quick fiber" produced from a modified corn milling process into ethanol and recovery of corn fiber oil. Journal of Applied Biochemistry and Biotechnology. 115:937-950.

SEPARATION OF BUTANOL FROM FERMENTATION BROTH BY PERVAPORATION: MASS FLUX AND ENERGY BALANCE - (Abstract Only)

Qureshi, N., Dien, B.S., Saha, B.C., Ezeji, T.C., Blaschek, H.P., Cotta, M.A. 2003. Separation of butanol from fermentation broth by pervaporation: mass flux and energy balance [abstract]. American Institute of Chemical Engineers. Paper No. 162B.

CONVERSION OF LIGNOCELLULOSE TO ETHANOL USING A RECOMBINANT E. COLI STRAIN - (Abstract Only)

Dien, B.S., Iten, L.B., Nichols, N.N., Cotta, M.A. 2003. Conversion of lignocellulose to ethanol using a recombinant E. coli strain [abstract]. Society of Industrial Microbiology. p. 102.

COMPARATIVE TOLERANCE STUDIES REVEAL DOSE-DEPENDENT INHIBITION OF ETHANOLOGENIC YEASTS BY FURFURAL AND 5-HYDROXYMETHYLFURFURAL - (Abstract Only)

Liu, Z., Slininger, P.J., Dien, B.S., Kurtzman, C.P., Gorsich, S.W. 2004. Comparative tolerance studies reveal dose-dependent inhibition of ethanologenic yeasts by furfural and 5-hydroxymethylfurfural [abstract]. Society of Industrial Microbiology. Paper No. 40.

BACTERIA ENGINEERED FOR FUEL ETHANOL PRODUCTION: CURRENT STATUS - (Review Article)

Dien, B.S., Cotta, M.A., Jeffries, T.W. 2003. Bacteria engineered for fuel ethanol production: current status. Applied Microbiology and Biotechnology. 63:258-266.

ISOLATION OF MICROORGANISMS FOR BIOLOGICAL DETOXIFICATION OF LIGNOCELLULOSIC HYDROLYSATES - (Peer Reviewed Journal)

Lopez, M.J., Nichols, N.N., Dien, B.S., Moreno, J., Bothast, R.J. 2004. Isolation of microorganisms for biological detoxification of lignocellulosic hydrolysates. Applied Microbiology And Biotechnology. 64:125-131.

INCREASING THE ETHANOL YIELD FROM A BUSHEL OF CORN; PROSPECTS FOR BIOMASS CONVERSION - (Abstract Only)

Dien, B.S., Bothast, R.J., Nichols, N.N., Cotta, M.A. 2003. Increasing the ethanol yield from a bushel of corn; prospects for biomass conversion [abstract]. International Starch Technology. Paper No. 2.

CONVERSION OF CORN FIBROUS MATERIAL INTO ETHANOL - (Abstract Only)

DIEN, B.S., NICHOLS, N.N., LI, X., COTTA, M.A., BOTHAST, R.J. CONVERSION OF CORN FIBROUS MATERIAL INTO ETHANOL. 25TH SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS. 2003. ABSTRACT P. 68.

POTENTIAL TO IMPROVE DRY MILL ECONOMICS BY INCREASING ETHANOL YIELD FROM CORN FIBER RESIDUE - (Abstract Only)

NAGLE, N., TUCKER, M.P., DIEN, B.S., COTTA, M.A., HICKS, K.B., MOREAU, R.A., SINGH, V., NGUYEN, Q. POTENTIAL TO IMPROVE DRY MILL ECONOMICS BY INCREASING ETHANOL YIELD FROM CORN FIBER RESIDUE. 25TH SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS SYMPOSIUM. 2003. ABSTRACT P. 20.

CORN FIBER HYDROLYSIS AND FERMENTATION TO BUTANOL USING CLOSTRIDIUM BEIJERINCKII BA101 - (Abstract Only)

EBENER, J.M., QURESHI, N., EZEJI, T.C., BLASCHEK, H.P., DIEN, B.S., COTTA, M.A. CORN FIBER HYDROLYSIS AND FERMENTATION TO BUTANOL USING CLOSTRIDIUM BEIJERINCKII BA101. 25TH SYMPOSIUM ON BIOTECHNOLOGY FOR FUELS AND CHEMICALS. 2003. PAPER NO. 2-14.

PRINCIPLES AND ECONOMICS OF PRETREATING CELLULOSE IN WATER FOR ETHANOL PRODUCTION - (Abstract Only)

Mosier, N., Hendrickson, R., Dreschel, R., Dien, B.S., Bothast, R.J., Welch, G., Ladisch, M.R. 2003. Principles and economics of pretreating cellulose in water for ethanol production [abstract]. American Chemical Society. Paper No. 103.

ENHANCEMENT OF ETHANOL YIELD FROM THE CORN DRY GRIND PROCESS BY FERMENTATION OF THE KERNEL FIBER FRACTION - (Peer Reviewed Journal)

Dien, B.S., Nichols, N.N., Iten, L.B., Bothast, R.J. 2004. Enhancement of ethanol yield from the corn dry grind process by fermentation of the kernel fiber fraction. In: Nelson, W.M., editor. Agricultural Applications in Green Chemistry. Chap. 6. Washington, DC:American Chemical Society. p. 63-77.

ENGINEERING LACTIC ACID BACTERIA WITH PYRUVATE DECARBOXYLASE AND ALCOHOL DEHYDROGENASE GENES FOR ETHANOL PRODUCTION FROM ZYMOMONAS MOBILIS - (Peer Reviewed Journal)

NICHOLS, N.N., DIEN, B.S., BOTHAST, R.J. ENGINEERING LACTIC ACID BACTERIA WITH PYRUVATE DECARBOXYLASE AND ALCOHOL DEHYDROGENASE GENES FOR ETHANOL PRODUCTION FROM ZYMOMONAS MOBILIS. JOURNAL OF INDUSTRIAL MICROBIOLOGY AND BIOTECHNOLOGY. 2003. V. 30. P. 315-321.

PRODUCTION OF ACETIC ACID BY DEKKERA/BRETTANOMYCES YEASTS UNDER CONDITIONS OF CONSTANT PH - (Peer Reviewed Journal)

FREER, S.N., DIEN, B.S., MATSUDA, S. PRODUCTION OF ACETIC ACID BY DEKKERA/BRETTANOMYCES YEASTS UNDER CONDITIONS OF CONSTANT PH. WORLD JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY. 2003. V. 19. P. 101-105.

REMOVAL OF FERMENTATION INHIBITORS FORMED DURING PRETREATMENT OF BIOMASS BY POLYMERIC ADSORBENTS - (Peer Reviewed Journal)

WEIL, J.R., DIEN, B.S., BOTHAST, R.J., HENDRICKSON, R., MOSIER, N.S., LADISCH, M.R. REMOVAL OF FERMENTATION INHIBITORS FORMED DURING PRETREATMENT OF BIOMASS BY POLYMERIC ADSORBENTS. INDUSTRIAL AND ENGINEERING CHEMISTRY RESEARCH. 2002. V. 41. P. 6132-6138.

ACETIC ACID PRODUCTION BY DEKKERA/BRETTANOMYCES YEASTS - (Abstract Only) - (15-Aug-02)

BIOCATALYSTS FOR PRODUCTION OF BIOETHANOL - (Abstract Only)

BOTHAST, R.J., NICHOLS, N.N., DIEN, B.S., COTTA, M.A. BIOCATALYSTS FOR PRODUCTION OF BIOETHANOL. SOCIETY OF INDUSTRIAL MICROBIOLOGY ANNUAL MEETING. 2002. PAPER NO. 12-1.

SCREENING AND DISCOVERY OF MICROORGANISMS FOR INHIBITOR ABATEMENT IN TOXIC FERMENTATION SUBSTRATES - (Abstract Only)

LOPEZ, M.J., NICHOLS, N.N., DIEN, B.S., MORENO, J., BOTHAST, R.J. SCREENING AND DISCOVERY OF MICROORGANISMS FOR INHIBITOR ABATEMENT IN TOXIC FERMENTATION SUBSTRATES. SOCIETY OF INDUSTRIAL MICROBIOLOGY ANNUAL MEETING. 2002. PAPER NO. 6-2.

PRE-TREATMENT OF WET-MILLED CORN FIBER TO IMPROVE RECOVERY OF CORN FIBER OIL, AND PHYTOSTEROLS - (Peer Reviewed Journal)

Cereal Chem. 80:118-122, 2003

FERMENTATION OF SUGAR MIXTURES USING ESCHERICHIA COLI CATABOLITE REPRESSION MUTANTS ENGINEERED FOR PRODUCTION OF L-LACTIC ACID - (Peer Reviewed Journal)

DIEN, B.S., NICHOLS, N.N., BOTHAST, R.J. FERMENTATION OF SUGAR MIXTURES USING ESCHERICHIA COLI CATABOLITE REPRESSION MUTANTS ENGINEERED FOR PRODUCTION OF L-LACTIC ACID. JOURNAL OF INDUSTRIAL MICROBIOLOGY AND BIOTECHNOLOGY. 2002. V. 29. P. 221-227.

IDENTIFICATION OF PSEUDOMONAS PUTIDA GENES INVOLVED IN DEGRADATION OF FURFURAL AND 2-FUROIC ACID - (Abstract Only) - (23-May-02)

SCREENING FOR MICROORGANISMS USEFUL IN DETOXIFICATION OF LIGNOCELLULOSIC HYDROLYSATES - (Abstract Only) - (23-May-02)

REMOVAL OF FURFURAL FORMED DURING BIOMASS PRETREATMENT BY POLYMERIC ADSORBENTS - (Abstract Only) - (01-May-02)

STRATEGIES FOR ENHANCED ETHANOL YIELD FROM CORN - (Abstract Only) - (01-May-02)

INFLUENCE OF CORN HYBRID ON ETHANOL PRODUCTION - (Peer Reviewed Journal)

DIEN, B.S., BOTHAST, R.J., ITEN, L.B., BARRIOS, L., ECKHOFF, S.R. FATE OF BT PROTEIN AND INFLUENCE OF CORN HYBRID ON ETHANOL PRODUCTION. CEREAL CHEMISTRY. 2002. V. 79. P. 582-585.

THE U.S. CORN ETHANOL INDUSTRY: AN OVERVIEW OF CURRENT TECHNOLOGY AND FUTURE PROSPECTS - (Review Article)

DIEN, B.S., BOTHAST, R.J., NICHOLS, N.N., COTTA, M.A. THE U.S. CORN ETHANOL INDUSTRY: AN OVERVIEW OF CURRENT TECHNOLOGY AND FUTURE PROSPECTS. INTERNATIONAL SUGAR JOURNAL. 2002. V. 104. P. 204-211.

IMPROVED BIOCATALYST FOR PRODUCTION OF BIOETHANOL AND LACTIC ACID - (Abstract Only)

DIEN, B.S., BOTHAST, R.J., NICHOLS, N.N., SKORY, C.D. IMPROVED BIOCATALYST FOR PRODUCTION OF BIOETHANOL AND LACTIC ACID. AMERICAN CHEMICAL SOCIETY. 2002. ABSTRACT NO. 322.

PRODUCTION OF L-LACTIC ACID USING RECOMBINANT ESCHERICHIA COLI - (Abstract Only) - (02-Aug-01)

FERMENTATION OF HIGH STARCH AND GMO CORN - (Abstract Only) - (02-Aug-01)

CONSTRUCTION OF AN OPERON FOR PRODUCTION OF ETHANOL IN LACTOCOCCUS LACTIS - (Abstract Only) - (02-Aug-01)

RECOMBINANT ESCHERICHIA COLI ENGINEERED FOR PRODUCTION OF L-LACTIC ACID FROM HEXOSE AND PENTOSE SUGARS - (Peer Reviewed Journal) - (13-Jul-01)

FATE OF THE BT PROTEIN IN DRY/WET MILLED FOR ETHANOL PRODUCTION - (Abstract Only) - (06-Jun-01)

METABOLIC ENGINEERING OF ESCHERICHIA COLI FOR IMPROVED PRODUCTION OF ETHANOL AND LACTIC ACID - (Abstract Only) - (09-May-01)

USE OF CATABOLITE REPRESSION MUTANTS FOR THE FERMENTATION OF SUGAR MIXTURES TO ETHANOL - (Peer Reviewed Journal) - (29-Dec-00)

METABOLIC ENGINEERING TECHNOLOGIES FOR IMPROVED PRODUCTION OF ETHANOL AND LACTIC ACID - (Abstract Only) - (19-Dec-00)

FERMENTATION OF SUGAR MIXTURES TO ETHANOL USING E. COLI CATABOLITE REPRESSION MUTANTS - (Abstract Only) - (25-May-00)

ACETIC ACID PRODUCTION BY BRETTANOMYCES YEAST - (Abstract Only) - (25-May-00)

PRODUCTION OF LACTATE AND ETHANOL FROM HEXOSE AND PENTOSE SUGARS USING RECOMBINANT ESCHERICHIA COLI - (Abstract Only) - (31-Mar-00)

GENETIC CONTROL OF METABOLIC FUNCTIONS FOR PRODUCTION OF FUELS AND CHEMICALS - (Abstract Only) - (31-Mar-00)

INCREMENTAL ETHANOL YIELDS FROM PROCESSING CORN FIBER BY THERMAL PRETREATMENT AND ENZYMATIC HYDROLYSIS - (Abstract Only) - (31-Mar-00)

XYLITOL PRODUCTION FROM CORN FIBER HYDROLYSATES BY A TWO-STAGE FERMENTATIONPROCESS - (Peer Reviewed Journal) - (11-Oct-99)

DEVELOPMENT OF NEW ETHANOLOGENIC ESCHERICHIA COLI STRAINS FOR FERMENTATION OF LIGNOCELLULOSIC BIOMASS - (Peer Reviewed Journal) - (20-Sep-99)

CONVERSION OF CORN FIBER TO FUEL ETHANOL: APPLICATIONS OF METABOLIC ENGINEERING TECHNOLOGIES - (Abstract Only) - (26-Aug-99)

PRODUCTION OF LACTATE FROM HEXOSE AND PENTOSE SUGARS USING RECOMBINANT ESCHERICHIA COLI - (Abstract Only) - (06-Aug-99)

MOLECULAR STRATEGIES FOR IMPROVED ETHANOL PRODUCING BACTERIA - (Abstract Only) - (06-Aug-99)

THE FUTURE OF FERMENTATIVE MICROORGANISMS - (Popular Publication) - (15-May-99)

CONVERSION OF CORN FIBER TO ETHANOL USING RECOMBINANT ESCHERICHIA COLI STRAINS - (Abstract Only) - (06-May-99)

FERMENTATIONS WITH NEW RECOMBINANT ORGANISMS - (Peer Reviewed Journal) - (05-May-99)

CONVERSION OF CORN FIBER TO ETHANOL BY RECOMBINANT E. COLI STRAIN FBR3 - (Peer Reviewed Journal) - (04-Mar-99)

REMOVAL OF FERMENTATION INHIBITORS BY POLYMERIC ADSORBENTS - (Abstract Only) - (27-Aug-98)

CANDIDA ARABINOFERMENTANS, A NEW L-ARABINOSE FERMENTING YEAST - (Peer Reviewed Journal) - (22-Jul-98)

DEVELOPMENT AND SCALE-UP OF A PROCESS FOR CONVERSION OF CORN FIBER INTO ETHANOL - (Abstract Only) - (03-Jun-98)

DEVELOPMENT AND SCALE-UP OF A PROCESS FOR CONVERSION OF CORN FIBER INTO ETHANOL - (Abstract Only) - (07-May-98)

FERMENTATION OF HEXOSE AND PENTOSE SUGARS USING A NOVEL ETHANOLOGENIC ESCHERICHIA COLI STRAIN - (Peer Reviewed Journal) - (27-Apr-98)

TWO-STAGE FERMENTATION OF CORN FIBER HYDROLYSATES FOR PRODUCTION OF XYLITOL - (Abstract Only) - (29-Mar-98)

GENETIC STABILITY OF METABOLIC ENGINEERED ESCHERICHIA COLI STRAINS FOR PRODUCTION OF ETHANOL - (Abstract Only) - (07-Aug-97)

FUEL ETHANOL PRODUCTION FROM CORN FIBER: CURRENT STATUS AND TECHNICAL PROSPECTS - (Review Article) - (25-Jul-97)

FERMENTABLE SUGARS FROM CORN BYPRODUCTS - (Proceedings) - (15-May-97)

FUEL ETHANOL PRODUCTION FROM CORN FIBER: CURRENT STATUS AND TECHNICAL PROSPECTS - (Abstract Only) - (08-May-97)

SEQUENTIAL FERMENTATION OF MIXED SUGARS FOR XYLITOL PRODUCTION - (Abstract Only) - (28-Apr-97)

A NOVEL METHOD FOR STABILIZING PLASMIDS IN CULTURES OF ESCHERICHIA COLI FORPRODUCTION OF ETHANOL AND L-LACTATE WITHOUT USING SELECTIVE MEDIUM - (Abstract Only) - (17-Apr-97)

CONVERSION OF CORN MILLING FIBROUS CO-PRODUCTS TO ETHANOL - (Peer Reviewed Journal) - (18-Oct-96)

FERMENTATION OF CORN FIBER SUGARS BY AN ENGINEERED XYLOSE UTILIZING SACCHAROMYCES YEAST STRAIN - (Peer Reviewed Journal) - (23-Sep-96)

PILOT SCALE CONVERSION OF CORN FIBER TO ETHANOL - (Abstract Only) - (17-Sep-96)

CONVERSION OF CORN FIBER TO ETHANOL - (Review Article) - (17-Sep-96)

STABILIZATION OF PET OPERON PLASMIDS AND ETHANOL PRODUCTION IN ESCHERICHIA COLI STRAINS LACKING LACTATE DEHYDROGENASE AND PYRUVATE-FORMATE LYASE ACTIVITIES - (Peer Reviewed Journal) - (09-Sep-96)

STABLE ETHANOL PRODUCTION BY PET OPERON-CONTAINING ESCHERICHIA COLI STRAINSLACKING LACTATE DEHYDROGENASE AND PYRUVATE-FORMATE LYASE - (Abstract Only) - (09-Aug-96)

CONVERSION OF CORN MILLING LOW-VALUE CO-PRODUCTS TO ETHANOL - (Abstract Only) - (18-Jul-96)

CONVERSION OF CORN MILLING LOW-VALUE CO-PRODUCTS TO ETHANOL - (Proceedings) - (18-Jul-96)

ETHANOL PRODUCTION FROM AFEX PRETREATED CORN FIBER BY RECOMBINANT BACTERIA - (Peer Reviewed Journal) - (04-Jul-96)

USE OF LOW-VALUE CORN MILLING FIBER MATERIALS FOR PRODUCTION OF ETHANOL ANDOTHER BIOPRODUCTS - (Abstract Only) - (31-May-96)

AFEX PRETREATMENT, ENZYMATIC HYDROLYSIS AND FERMENTATION OF GLUCOSE AND XYLOSE FROM CORN FIBER BY SACCHAROMYCES STRAIN (1400PLNH32) - (Abstract Only) - (09-May-96)

SCREENING FOR L-ARABINOSE FERMENTING YEASTS - (Peer Reviewed Journal) - (07-Aug-95)

CONVERSION OF MULTIPLE SUBSTRATES TO ETHANOL - (Abstract Only) - (26-Jan-95)