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United States Department of Agriculture

Agricultural Research Service

WAYNE B. HUNTER

Research Entomologist

Wayne Hunter,Ph.D.
Research Entomologist

Adult Glassy-winged Sharpshooter Leafhopper, GWSS, Homalodisca coagulata (Say). Vector of Pierce's Disease of grapes, a xylem-limited bacterium, Xylella fastidiosa. Adults are about ½ inch, (12 mm) long.
U. S. Horticultural Research Laboratory
2001 South Rock Road
Fort Pierce, FL 34945
Telephone: (772) 462-5898
Fax: (772) 462-5986
Email:
Wayne.Hunter@ars.usda.gov

Adult Glassy-winged Sharpshooter Leafhopper, GWSS, Homalodisca vitripennis Germar,. Vector of Pierce's disease of grapes, a xylem-limited bacterium, Xylella fastidiosa.  Adults are about 1/2 inch, (12 mm) long.

Current Research Projects:      

Dr. Hunter, Lead Scientist conducting research on the spread of plant diseases by insects.The main focus of research is on the glassy-winged sharpshooter, GWSS, and other leafhoppers which spread the plant infecting bacteria, Xylella fastidiosa,at the U.S. Horticultural Research Lab, Fort Pierce, Florida.  Dr. Hunter works to provide solutions to insect problems under USDA-ARS National Program 304, Crop Protection and Quarantine.  Dr. Hunter’s lab is elucidating GWSS biology using a genomics approach and has produced and published ~25,000 genetic sequences isolated from the GWSS.  These can be found at the National Center Biotechnology and Information, NCBI, expressed sequence tag, EST database for access by the research and education community.   Continuing examination of GWSS proteins have led to a deeper understanding of leafhopper feeding, development, and pathogen interactions.  He is the founder of the ‘Southeastern Xylella Sharpshooter Initiative’ aimed at increasing interactions among researchers on Pierce’s disease and leafhoppers.  Dr. Hunter is also examining the use of insect viruses as a possible management tools to reduce insect populations, such as GWSS.   He has discovered several new insect viruses isolated from aphids, leafhoppers, psyllids, and fire ants.

Dr. Hunter also conducts research on exotic insect pests.  He has produced over 120,000 sequences from many different insect species:   Aphids, ( Toxoptera citricida, Acyrthosiphon pisum, Aphis gossypii); Leafhoppers, ( Homalodisca coagulata, Oncometopia nigricans); Asian Citrus Psyllid, ( Diaphorina citri); Pink hibiscus mealybug, ( Maconellicoccus hirsutus); citrus weevil, ( Diaprepes abbreviatus); Asian longhorn beetle, ( Anoplophora glabripennis); and parasitoids ( Lysiphlebus testaceipes, Nasonia).   He was awarded ‘Entomologist of the Year 2005’ from the Florida Entomological Society for, “Internationally recognized contributions to insect genomics”.  He is a member of the International Aphid Genomics working group, working with researchers from the USA, France, Spain, Japan, and Australia.   The aphid genome effort has resulted in the production of two pea aphid microarrays which are being used to increase our understanding of gene expression in aphid biology.   Dr. Hunter is also a member of the Genome Steering committee for the National Parasitoid Genome Project, Nasonia and the International Whitefly Genome Initiative.   He also organizes the ‘International Insect Genomics Workshop at the International Plant & Animal Genome conference.   Dr. Hunter is a member of the ‘International Grape Genome Program’, and a co-developer of the ‘North American Grape Genome Database’, which is hosted at FloridaA&MUniversity.   North American grape varieties have greater resistance to grape diseases and insect pests.   Design of genetic markers linked to resistance traits are being used in a maker-assisted selection program to produce new grape varieties with resistance to important grape diseases, such as Pierce's Disease, and fungal pathogens.

Due to the economic importance of the Asian citrus psyllid, Diaphorina citri, as a vector of bacteria associated with the disease Huanglongbing, HLB in citrus, several new genomics efforts have been started to address many of the unknowns surrounding the cause and spread of HLB.

                      

International Asian Citrus Psyllid Genome Consortium
Hunter, Wayne B. (Lead) USA

National Program Staff:
Dr. Kevin Hackett, NPL, ARS, Beltsville, MD (Insects)
Dr. Gail Wisler, NPL, ARS, Beltsville, MD (Plant Disease)

Steering Committee:
Dr. Susan Brown, KSU, Arthropod Genomics Consortium, KS
Dr. Stephen Richardson, Human Genome Sequencing Center, Baylor Univ., TX
Dr. Jay Evans, USDA, ARS, Honey bee genomics, Beltsville, MD

Working Group Members:contact Wayne.Hunter@ars.usda.gov

  Project ID: 29473
  Project Status: Accepted
  Project Type:   Single Species Project
  Lead Contact's Name:   Wayne Hunter, Wayne.hunter@ars.usda.gov
  Submitting Organization:   USDA, ARS, U.S.Hort. Res. Lab, Ft. Pierce, FL
  Contact's Name:   Robert Shatters, Jr., Robert.shatters@ars.usda.gov
  Contact's Name:   David Hall, David.hall@ars.usda.gov
  Submitting Organization:   USDA, ARS, U.S.Hort. Res. Lab, Ft. Pierce, FL
  Locus Tag Prefix:   DIACI
  Source of DNA:   W. Hunter, Colonies, U.S.Horticultural Res. Lab
    2001 South Rock Road, Ft. Pierce, FL, USA .

International Diaphorina citri Wolbachia Genome Consortium
Magdalen Lindeberg, Cornell University, NY (Lead researcher)
Surya Saha, Cornell University, NY

Wayne Hunter, USHRL, ARS, Ft. Pierce, FL (co-organizer)

Justin Reese, Genformatics, LLC, Alpharetta, GA

Project Titles:

·          International Asian Psyllid Genome Consortium, Psyllid genome/transcriptome data, www.psyllid.org ,  Diaphorina citri, (Hemiptera: Psyllidae) Asian citrus psyllid, vector of bacterial pathogens associated with Citrus Greening Disease, Huanglongbing.

·          International Leafhopper Genome Consortium, i5K project: Glassy-winged sharpshooter, H. vitripennis, (Hemiptera: Cicadellidae) vector of Xylella fastidiosa, pathogen causing Pierce’s Disease of Grapevine.

·          Development of RNA interference, RNAi, strategies to reduce insect pests and disease spread.

·          Development of leafhopper and psyllid cell cultures.

·          Micro organism identification and cultivation from psyllids and leafhoppers (bacteria and viruses).

 

Select Publications (Last 10 Years): (* postdoctoral research associate author; **Student author) 

 

1.  Hunter, W.B., Lapointe, S. L., and Dang, P.  2002. Iridovirus infection and topical application to whitefly. Proc. Florida State Hort. Soc. 114:64-67. (Proceedings, Peer-Reviewed).

2.  Hunter, W.B., Dang, P.M., Bausher, Chaparro, J.X., McKendree, W., Shatters, R.G. Jr., McKenzie, C.L., Sinisterra, X.H.  2003. Aphid Biology: Expressed Genes from Alate Toxoptera citricida (Kirkaldy), Brown Citrus Aphid (Hemiptera: Aphididae).  Journal of Insect Science. http://www.insectscience.org/3.23

3.  Hunter, W.B., Lapointe, S.L., Sinisterra, X.H., Achor, D.S., Funk, C.J.  2003.  Iridovirus in Diaprepes Root Weevils (Coleoptera: Curculionidae: Diaprepes abbreviatus).  Journal of Insect Science 3:9, Available: http://www.insectscience.org/3.9

4.  Hunter, W.B., and SL. Lapointe. 2003.  Iridovirus Infection of a Cell Culture from the Diaprepes Root Weevil [Coleoptera: Curculionidae: Diaprepes abbreviatus (L.)].  Journal of Insect Science, http://www.insectscience.org/3.37.

5.  Lapointe, S.L., McKenzie, C.L. and W.B. Hunter. 2003. Toxicity and repellency of Tephrosia candida to larval and adult Diaprepes root weevil (Coleoptera: Curculionidae). J. Econ. Entomol. 96(3): 811-816.

 

6.  Bausher M, Shatters R, Chaparro J, Dang P, Hunter W.B., Niedz R.  2003. An expressed sequence tag (EST) set from Citrus sinensis L. Osbeck whole seedlings and the implications of further perennial source investigations.  Plant Science 165 (2): 415-422.

 

7.  Shatters, R.G, Jr., Bausher, M.G,  Hunter, W.B., Chaparro, J.X., Dang P.M, Niedz, R.P., Mayer, R.T., McCollum, T.G., and X.H. Sinisterra. 2003.  Putative protease inhibitor gene discovery and transcript profiling during fruit development and leaf damage in grapefruit (Citrus paradise Macf.).  GENE 326: 77-86.

 

8.  Niedz, R.P., Shatters, R.G., Bowman, K.D., McCollum, G., Bausher, M.G., Hunter, W.B., and Chaparro, J.X. 2003.  The role of genomics in citrus improvement.  Proceedings of the Florida State Horticultural Society 116: 278-282. 

 

9.  Lapointe, S.L., W.B. Hunter, and R.T. Alessandro.  2004.  Cuticular hydrocarbons on elytra of the Diaprepes root weevil Diaprepes abbreviatus (L.) (Coleoptera: Curculionidae).  Agricultural and Forest Entomology 6: 1-7.

10.  Valles, S.M., C.A. Strong, P.M. Dang, W.B. Hunter, R.M. Pereira, D.H. Oi, A.M. Shapiro, D.F. Williams. 2004. A picorna-like virus from the red imported fire ant, Solenopsis invicta: Initial discovery, genome sequence, and characterization. Virology 328: 151-157.

11.  McKenzie, C.L., A.A. Weathersbee III, W.B. Hunter, and G.J. Puterka.  2004.  Sucrose octanoate toxicity to brown citrus aphid (Homoptera: Aphididae) and the parasitoid Lysiphlebus testaceipes (Hymenoptera: Aphidiidae).  Journal of Economic Entomology 97: 1233-38.

    12.  Tipping, C., R., Mizell, B., Brodbeck, P. Andersen, W.B. Hunter and E. R. Lopez-Gutierrez.2005. A novel method to induce oviposition of the GWSS (Hemiptera: Auchenorrhyncha: Cicadellidae).  J. Entomol. Sci. 40(2) 246-249.

    13.  *Sinisterra, X.H., C.L. McKenzie, W.B. Hunter, and R.G. Shatters Jr.  2005. Differential transcriptional activity of plant pathogenic begomoviruses in their whitefly vector (Bemisia tabaci, Gennadius: Hemiptera Aleyroididae). J. General Virology 86: 1525-32.

    14.  **Mozoruk, J, **Hunnicutt, L.E, Cave, R.D, Hunter, W.B., Bausher, MG. 2006. Profiling transcriptional changes in Citrus sinensis (L.) Osbeck challenged by herbivory from the xylem-feeding leafhopper Homalodisca coagulata (Say) by cDNA macroarray analysis. Plant Science 170:1068-1080.

      15.  Hunter, W.B., *Katsar, C.S, Chaparro, J.X. 2006. Nucleotide sequence of 3′ -end of Homalodisca coagulata Virus-1. A new leafhopper-infecting virus from the glassy-winged sharpshooter.  J. Insect Science 6.28. Available online: insectscience.org/6.28/.

        16.  *Hunnicutt, L.E., Hunter, W.B., Cave, R.D., Powell, C.A., Mozoruk, J.J. 2006.  Complete Genome Sequence and Molecular Characterization of Homalodisca coagulata virus-1, a novel virus discovered in the glassy-winged sharpshooter (Hemiptera: Cicadellidae). Virology 350:67-78.

        17.  Lu, J., Huang, H., Ren, Z., Bradeley, F., Hunter, W.B. 2006. Comparative Genomics Analysis between V. shuttleworthii and V. vinifera grapes. Acta Horticulturae 736. 

          18.  *Huang, H., Lu, J., Hunter, W.B.  2006. Comparative Sequence and Functional Analysis of Stilbene Synthase Genes among Vitis Species. Acta Horticulturae 736.

          19.  *Huang, Z., Hunter, W.B., Cleland, C.A, Wolinsky, M., Lapointe, S.L., Powell, C.A. 2006. A new member of the growth-promoting glycoproteins from Diaprepes root weevil (Coleoptera: Curculionidae).  Florida Entomologist 89(2): 223-232.

           

          20.  Wilson, A.C., Dunbar, H.E., Davis, G.K., Hunter, W.B., Stern, D.L., Moran, N.A. 2006. A dual-genome microarray for the pea aphid, Acyrthosiphon pisum, and its obligate bacterial symbiont, Buchnera aphidicola. BMC Genomics 2006, 7:50 (14 Mar 2006).

          21.  Sabater-Munoz, Legeai, Rispe, Bonhomme, Dossat, Duclert, Gauthier, Giblot-Ducray, Hunter, W.B., Dang, Matinez-Torres, Moya, Nakabachi, Prunier-Leterne, Rahbe, Simon, Stern, Wincker, Tagu. 2006.  Large-scale gene discovery in the pea aphid Acyrthosiphon pisum (Hemiptera). BMC Genome Biology 7:R21 (doi:10.1186/gb-2006-7-3-r21).

          22.  Sinisterra, X.H., Shatters, R.G., Jr, Hunter, W.B., Powell, C.A., McKenzie, C.L. 2006. Longevity of ingested mRNA transcripts in the gut of a homopteran (Bemisia tabaci): avoiding experimental artifacts.  Entomologia Experimentalis et Applicata. (DOI: 10.1111/j.1570-7458.2006.00473.x).

          23.  Katsar, C.S., Hunter, W.B., Sinisterra, X.H. 2007. Phytoreovirus-like sequences from glassy-winged sharpshooter salivary glands. Florida Entomologist 90(1):196-203. 

            24.  Coudron, T.A., Brandt, S.L., Hunter, W.B. 2007.  Molecular profiling of proteolytic and lectin transcripts in Homalodisca vitripennis (Hemiptera: Auchenorrhyncha: Cicadellidae) feeding on sunflower and cowpea. Archives of Insect Biochemistry and Physiology 65: Online: DOI: 10.1002/arch.20200

            25.  *Mozoruk, J., **Hunnicutt, L.E, Hunter, W.B., Bausher, M.G. 2007. CsV03-3 is a member of a novel gene family from citrus that encodes a protein with DNA binding activity and whose expression is responsive to defense signals and abiotic stress. J. Plant Physiology  (doi: 10.1016/j.jplph.2007.01.009).

             

            26.  Boykin, LM, Bagnall, RA, Frohlich, DR, Hall, DG, Hunter, W.B., Katsar, CS, McKenzie, CL, Rosell, RC, Shatters, Jr, RG. 2007.  Twelve polymorphic microsatellite loci from the Asian citrus psyllid, Diaphorina citri Kuwayama, the vector for citrus greening disease, Huanglongbing.  Molecular Ecology Notes: online (doi: 10.111/j1471-8286.2007.01831.x).

            27.  Niehuis, O, Judson, AK, Werren, JH, Hunter, W.B., Dang, PM, Dowd, SE, Grillenberger, B, Beukeboom, LW, Gädau, J. 2007. Species-diagnostic Single-Nucleotide Polymorphism and sequence-tagged site markers for parasitic wasp genus Nasonia (Hymentopera; Pteromalidae). J. Econ. Entomol. 100:1033-1036.

              28.  Hunnicutt, L.E., Mozoruk, J.J., Hunter, W.B., Crosslin, J.M., Cave, R.D., Powell, C.A. 2007. Prevalence and natural host range of Homalodisca coagulata virus-1 (HoCV-1). Archives of Virology 152(12): online (DOI: 10.1007/s00705-007-1066-2).

              29.  Tipping, C, Mizell III, R.F., Andersen, P.C., Brodbeck, B.V., Hunter, W.B. 2007.  A Behavioral Model for the Glassy-winged Sharpshooter, Homalodisca vitripennis:  Optimization of Host Plant Utilization in a Risky Environment. (Accepted Oct. 07, J. Econ. Entomol.)

              30.  Lu, J. H. Huang, Z. Ren. F. Bradeley, W. Hunter, W.B., P. Dang. 2007. Towards identification, isolation and characterization of disease resistance genes from native north American grape (Vitis L.) species. Acta Horticulturae, 738:767-772. 

              31.  *Huang, H., Lu, J., Hunter, W.B. 2007. Comparative Analysis of Stilbene Synthase Genes among Vitis Species.  Acta Horticulturae. 738:755-758.  

              32.  *Katsar, CS, Hunter, W.B., Sinisterra, XH. 2007. Phytoreovirus-like sequences from glassy-winged sharpshooter salivary glands. Florida Entomologist 90(1):196-203. 

              33.  Coudron, TA,  Brandt, SL,  Hunter, W.B. 2007.  Molecular profiling of proteolytic and lectin transcripts in Homalodisca vitripennis (Hemiptera: Auchenorrhyncha: Cicadellidae) feeding on sunflower and cowpea. Archives of Insect Biochemistry and Physiology 66:76-88.

              34.  Boykin, LM, Bagnall, RA, Frohlich, DR, Hall, DG, Hunter, W.B., Katsar, CS, McKenzie, CL, Rosell, RC, Shatters, Jr, RG. 2007.  Twelve polymorphic microsatellite loci from the Asian citrus psyllid, Diaphorina citri Kuwayama, the vector for citrus greening disease, Huanglongbing.  Molecular Ecology Notes: online (doi: 10.111/j1471-8286.2007.01831.x). 

              35.  Niehuis, O, Judson, AK, Werren, JH, Hunter, W.B., Dang, PM, Dowd, SE, Grillenberger, B, Beukeboom, LW, Gadau, J. 2007. Species-diagnostic Single-Nucleotide Polymorphism and sequence-tagged site markers for parasitic wasp genus Nasonia (Hymentopera; Pteromalidae). J. Econ. Entomol. 100:1033-1036. 

              36.  Polston, JE., Hiebert, E., Abouzid, A.M., Hunter, W.B.2008. “Materials and methods for producing geminivirus resistant plants” Patent No. 7,294,758.

                37.  Valles, SM, Pereira, RM, Hunter, W.B., Oi, DH, Strong, CA, Dang, PM, Williams, DF. 2008. “Solenopsis invicta Viruses”.  Serial Number 11/239,183. (Patent 2008).

                38.  *Hunnicutt, LE, Mozoruk, JJ, Hunter, W.B., Crosslin, JM, Cave, RD, Powell, CA. 2008. Prevalence and natural host range of Homalodisca coagulata virus-1 (HoCV-1). Archives of Virology 153: 61-67.  Online (DOI: 10.1007/s00705-007-1066-2).

                  39.  Mozoruk, J, Hunnicutt, LE, Hunter, W.B., Bausher, MG. 2008. CsV03-3 is a member of a novel gene family from citrus that encodes a protein with DNA binding activity and whose expression is responsive to defense signals and abiotic stress. Journal of Plant Physiology, 165 (5): 531-543.  doi:10.1016/j.jplph.2007.01.009.

                  40.  Mizell, RF, Tipping, C, Andersen, PC, Brodbeck, BV, Hunter, W.B., Northfield, T. 2008. Behavioral model for Homalodisca vitripennis (Hemiptera: Cicadellidae): Optimization of host plant utilization and management implications. Environ. Entomol. 37:1049-1062./

                  41.  Hall, DG.,Hunter, W.B. 2008. Populations of sharpshooters in two citrus groves in East-central Florida as indicated by yellow sticky card traps. Florida Entomologist 91(3): 488-490. 

                  42.  Valles, SM, Strong, CA, Hunter, W.B., Dang, PM, Pereira, RM, Oi, DH, Williams, DF. 2008. Expressed sequence tags from the red imported fire ant, Solenopsis invicta: Annotation and utilization for discovery of viruses. Journal of Invertebrate Pathology 99:74-81.

                  43.  Hunter, W.B., Smith, MT, Hunnicutt, LE.  2009. Analysis and functional annotation of expressed sequence tags from the Asian longhorned beetle, Anoplophora glabripennis. J. Insect Science. 9/39.

                   

                  44.  Northfield,TD.,  Mizell, RF., Paini, DR., Andersen, PC., Brodbeck, BV., Riddle, TC., Hunter, W.B. 2009. Dispersal, Patch Leaving, and Distribution of Homalodisca vitripennis (Hemiptera: Cicadellidae). Environ. Entomol. 38(1): 183-191.

                  45.  Stenger, DC, Sisterson, MS, Krugner, R, Backus, EA, Hunter, W.B. 2009. A New Phytoreovirus Infecting the Glassy-Winged Sharpshooter (Homalodisca vitripennis) Virology 386:469-477.

                   

                  46.  Hunter, W.B., Shelby, KS, Boykin, LM, Dang, PM, Weathersbee, AA. 2009. Ribosomal proteins and expressed sequence tags from Lysiphlebus testaceipes (Hymenoptera: Aphidiidae). Florida Scientist 72:196-207.

                  47.  *Marutani-Hert, M., Hunter, W.B., Katsar, CS, Sinisterra, XH., Hall, DG.,  Powell, CA. 2009. Reovirus-like sequences isolated from adult Asian citrus psyllid, (Hemiptera: Psyllidae: Diaphorina citri).  Florida Entomologist 92:314-320.

                    48.  Hunter, W.B., Dowd, SE, Katsar, CS, Shatters, Jr, RG, McKenzie, CL, Hall, DG. 2009. Psyllid biology: expressed genes in adult Asian citrus psyllids, Diaphorina citri Kuwayama. The Open Entomology Journal 3: 18-29.

                      49.  *Marutani-Hert, M, Hunter, W.B., Hall, DG. 2009. Establishment of Asian Citrus Psyllid (Diaphorina citri) Cell Lines. In Vitro Cellular & Developmental Biology-Animal. 45:317-320.  DOI 10.1007/s11626-00909188-3.

                      50.  Avery, P. B., Hunter, W. B, Hall, D. G., Jackson, M. A., Powell, C. A. and M. E. Rogers 2009. Diaphorina citri (Hemiptera: Psyllidae) infection and dissemination of the entomopathogenic fungus Isaria fumosorosea (Hypocreales: Cordycipitaceae) under laboratory conditions. Florida Entomologist 92: 608-618.

                        51.  *Huang, H., Lu, J., Hunter, W.B.., Dowd, S., Katsar, C., Jörgenson, C. 2009. Development of a grape genomics database using IBM DB2 content manager software. Acta Horticulturae 827: 87-92.

                          52.  **Schreiber, H., Hail, D., Hunter, W.B., Bextine, B. 2009. Phylogenetic analysis of heat shock proteins in glassy-winged sharpshooter, Homalodisca vitripennis. Southwestern Entomologist 34:457-468.

                            53.  *Oliveira, DCSG., Hunter, W.B..,  Ng, J., Desjardins, CA., Dang, PM., JH. Werren. 2010. Data mining cDNAs reveals three new single stranded RNA viruses in Nasonia (Hymenoptera: Pteromalidae). Insect Mol. Biol. 19:99-107. DOI 10.1111/j.1365-2583.2009.00934.x

                              54.  The International Aphid Genomics Consortium. 2010. Genome Sequence of the Pea Aphid Acyrthosiphon pisum. Ins. Mol.Biol. 19 (Special edition).

                              55.  The International Aphid Genomics Consortium. 2010. Genome Sequence of the Pea Aphid Acyrthosiphon pisum. PLoS Biol. 8(2): e1000313. doi:10.1371/journal.pbio.1000313
                              02/23/10 published.

                                56.  Nasonia Genome Working Group. 2010. Functional and Evolutionary insights from the genomes of three parasitoid Nasonia species. Science 327(5963):343-348.

                                57.  International Nasonia Genome Consortium. 2010. (Special Issue: The Nasonia Genome.) Insect Molecular Biology 19(1): 1-187. Journal compilation © 2009 Royal Entomological Society. Online ISSN: 1365-2583, Print ISSN: 0962-1075.

                                58.  Hunter, W.B.. 2010. Medium for development of bee cell cultures (Apis mellifera: Hymenoptera: Apidae). In Vitro Cell. Dev. Biol.--Animal 46:83-86.

                                59.  Hunter, W.B., Shelby, KS, Purcell, AH, Hunnicutt, LE. 2010. Protein identities- Graphocephala atropunctata expressed sequence tags: Expanding leafhopper vector biology.  Florida Scientist 73:89-98.

                                60.  **Tufts, DM, Hunter, W.B., Bextine, B. 2010. Discovery and effects of Texas Solenopsis invicta virus [SINV-1 (TX5)] on red imported fire ant populations. Journal Invertebrate Pathology

                                61.  *Marutani-Hert, M., Hunter, W.B.., Hall, D.G. 2010. Gene response to stress in the Asian citrus psyllid (Hemiptera: Psyllidae). Florida Entomologist 93: 519-525.

                                62.  Hunter, W.B., Ellis, J, vanEngelsdorp, D, Hayes, J, Westervelt, D, Glick, E, Williams, M, Sela, I, Maori, E, Pettis, J, Cox-Foster, D, Paldi, N.2010. Large-Scale Field Application of RNAi Technology Reducing Israeli Acute Paralysis Virus Disease in Honey Bees (Apis mellifera, Hymenoptera: Apidae). PLoS Pathog 6(12): e1001160. doi:10.1371/journal.ppat.1001160.

                                63.  Umar, G., Vasanthaiah, HK, Kambiranda, D., Basha, S.M., Phills, B.R., Hunter, W.B.. 2010. Assessment of genetic diversity among selected raspberry cultivars. Proc. Fla. State Hort. Soc. 123:26-28. (peer-reviewed proceedings).

                                64.  **Hail, D., Hunter, W.B.., Dowd, S.E., Bextine, B.R. 2010. Expressed Sequence Tag (EST) Survey of Life Stages of the Potato Psyllid, Bactericera cockerelli, using 454 Pyrosequencing. Southwestern Entomologist, 35(3):463-466.

                                65.  Huang, H., Lu, J., Ren, Zhongbo, R., Hunter, W.B.., Dowd, SE, Dang, P. 2010. Mining and validating grape (Vitis L.) ESTs to develop EST-SSR markers for genotyping and mapping. Mol. Breeding 28(2): 241-254.

                                66.  Avery, P.B., Hunter, W.B., Hall, D.G., Jackson, M.A., Powell, C.A., Rogers, M.E. 2009. Diaphorina citri (Hemiptera: Psyllidae) Infection and Dissemination of the Entomopathogenic Fungus Isaria fumosorosea (Hypocreales: Cordycipitaceae) Under Laboratory Conditions.  Florida Entomologist 92:608-618.

                                67.  Huang H., Lu  J.,  Hunter W.B.., Liang S. 2011. Using IBM Content Manager for genomic data annotation and quality assurance tasks.  IBM Journal of Research and Development , 55(6),(DOI: 10.1147/JRD.2011.2172837).

                                 

                                68.  Jenkins DA, Hunter W.B., Goenaga R. 2011. Effects of Invertebrate Iridescent Virus 6 in Phyllophaga vandinei and its potential as a biocontrol delivery system. Journal of Insect Science 11:9 available online: insectscience.org/11.9.

                                  69.  **Tufts, D.M., Spencer, K.,Hunter, W.B.., Bextine, B.R. 2011. Delivery system using sodium alginate virus loaded pellets to red imported fire ants (Solenopsis invicta, Hymenoptera: Formicidae). Florida Entomologist  94(2):237-241.

                                    70.  **Welch, E.W., Hunter, W.B., Shelby, K.S., Mizell, R.F., Tipping, C., Katsar, C.S., Bextine, B.R. 2011. Leafhopper comparative genomics-Identifying similarities and differences across leafhopper vectors of Xylella fastidiosa. Southwestern Entomol. 36: 305-321.

                                      71.  Avery, P.B., Wekesa, V.W., Hunter, W.B., Hall, D.G., McKenzie, C. L., Osborne, L.S., Powell, C.A., Rogers, M.E. 2011. Effects of the fungus Isaria fumosorosea (Hypocreales: Cordycipitaceae) on reduced feeding and mortality of the Asian citrus psyllid, Diaphorina citri (Hemiptera: Psyllidae). Biocontrol Science and Technology 21: 1065-1078.

                                        72.  Hunter, W.B., Avery, P. B., Pick, D, and C. A. Powell. 2011. Broad spectrum potential of Isaria fumosorosea on insect pests of citrus. Florida Entomologist 94: 1051-1054.

                                          73.  Moran, P.J., Patt, J.M., Cabanillas, H.E., Adamczyk, J.L., Jackson, M.A., Dunlap, C.A, Hunter, W.B., Avery. P.B. 2011. Localized autoinoculation and dissemination of Isaria fumosorosea for control of the Asian citrus psyllid in South Texas. Subtropical Plant Science 63:23-35.

                                            74.  Hunter, W.B., Glick, E, Paldi, N, Bextine, BR. 2012. Advances in RNA interference: dsRNA Treatment in Trees and Grapevines for Insect Pest Population Suppression. Southwestern Entomologist 37(1):85-87.

                                              75.  Hunter, W.B.., Powell, C.A., Hall, D.G. 2012. Entomopathogenic fungus, Isaria fumosorosea, and aphid parasitoid, Lysiphlebus testaceipes, for managing infestations of Brown Citrus Aphid, Toxoptera citricidus. 95(3): 764-766.

                                                76.  Boykin, L.M., De Barro, P., Hall, D.G., Hunter, WB., McKenzie, C.L., Powell, C.A., Shatters, R.G..  2012. Overview of worldwide diversity of Diaphorina citri Kuwayama mitochondrial cytochrome oxidase 1 haplotypes: Two old world lineages and a new world invasion. Bull Entomol Res. 17:1-10.

                                                  77.  **Arras, J., Hunter, W.B., Bextine, B.R. 2012. Comparative analysis of antennae sensory arrays in Asian citrus psyllid, Diaphorina citri, and potato psyllid Bactericera cockerelli (Hemiptera). Southwestern Entomol. 37:1-12.

                                                   

                                                  78.  Genersch, E., Gisder, S., Hedtke, K., Hunter, W.B., Möckel, N., Müller, U. 2012. Cell culture techniques in honey bee research. COLOSS:Beebook, Journal of Apicultural Research 51(2): 151-153.

                                                   

                                                  79.  Mizell, R.F., Andersen, P.C., Brodbeck, B.V., Hunter, W.B. 2012. Congener response reduces risks from bottom-up and top-down forces: Behavioral parsimony by a xylophage. American Entomol. 58: 106-115.

                                                   

                                                  80.     Burand, J.P., Hunter, W.B. 2012. RNAi: Future in insect management. J. Invertebr. Pathol.  http://dx.doi.org/10.1016/j.jip.2012.07.012.

                                                   

                                                  81.     Saha, S., Hunter, W.B., Reese, J., Morgan, J.K., Marutani-Hert, M., Huang, H., Lindeberg, M. 2012.  Survey of Endosymbionts in the Diaphorina citri Metagenome and Assembly of a Wolbachia wDi Draft Genome. PLoS ONE 7(11): e50067. doi:10.1371/journal.pone.0050067

                                                   

                                                  82.     Genersch, E., Gisder, S., Hedtke, K., Hunter, W.B., MOckel, N., Müller, U. 2012. Cell culture techniques in honey bee research. Journal of Apicultural Research 51(1):2013. COLOSS: Beebook.  DOI 10.3896/IBRA.1.52.1.02. 

                                                   

                                                  (UPDATE   DATASETS AVAILABLE  IN NCBI”)

                                                  Published Public Access Insect Genetic Datasets-NCBI

                                                   

                                                  1.     Bausher, M., McKendree, W., Dang, P., Chaparro, J., Shatters, R., Hunter, W.B., Niedz, R 2002.  Published 2,306 electronic pages of “EST high quality sequences from a Citrus sinensis (sweet orange) whole seedling cDNA library” NCBI EST database.  http://www4.ncbi.nlm.nih.gov/ (Public Database).

                                                   

                                                  2.     Hunter, W.B. 2003.  Published 4,320 electronic pages:  Adult Alate Brown Citrus Aphid Toxoptera citricida cDNA clones.  U.S. Horticultural Research Laboratory, 2001 South Rock Rd., Fort Pierce, FL 34945, USA.  Accession no. CB832665-CD452353. NCBI.  (Public Database).

                                                   

                                                  3.     Hunter, W.B. 2003.  Published 427 electronic pages.  Data Set: Glassy-winged Sharpshooter, Expressed Sequence Tags, ESTs, from Adult Homalodisca coagulata (Say) (Hemiptera: Cicadellidae).  GenBank Accession Numbers CF194966 through CF195393.  National Center for Biotechnology Information, NCBI.  (Public Database).

                                                   

                                                  4.     Hunter, W.B., and Dang, P.M.  2003.  Published 970 electronic pages:  Gene expression in Acyrthosiphon pisum (Hemiptera: Aphididae).  U.S. Horticultural Research Laboratory, 2001 South Rock Rd., Fort Pierce, FL 34945, USA.  Accession no. CF587442-CF588411.  NCBI.  (Public Database).

                                                   

                                                  5.     Lu, J., and Hunter, W.B., Dang, P.M., Huang, H.  2003-2004.  Published 218 electronic pages:  Genes expressed in Vitis shuttleworthii. Grapes.  10,600 EST’s. Florida A&M University, Tallahassee, USDA, ARS.  Acc. No. CF568781- CV100862, NCBI.(Public Database).

                                                   

                                                  6.     Sabater-Munoz, B, Legeai, F, Bonhomme, J, Dang, P, Dossat, C, Duclert, A., Gauthier, J.P., Hunter, W.B., Martinez-Torres, D., Moya, A., Nakabachi, A., Prunier-Leterme, N, Rahbe, Y., Shigenobu, S., Simon, J.C., Stern, D, Wincker, P, Tagu, D. 2004. Annotated ESTs of the Pea Aphid.  21,588 sequences from Acyrthosiphon pisum. Acc. nos:  CV850040-CV828453.  NCBI. (PubMed).

                                                   

                                                  7.     Hunter, W.B., Martinez-Torres, D., Rahbe, Y., Sabater-Munoz, B., Stern, D., Tagu, D., Wincker, P. 2004. An expressed sequence tag database for the pea aphid Acyrthosiphon pisum. 15,514 sequences Acc. No. CN7664460-CN748946. (Public Database).

                                                   

                                                  8.     Lapointe, S.L., Hunter, W.B. and Dang, P.M. 2004.  Gene expression in Diaprepes abbreviatus (Coleoptera: Curculionidae). 3,546 sequences, Acc. no. CN488395-CN473751. National Center for Biotechnology Information, NCBI.  (Public Database).

                                                   

                                                  9.     Hunter W.B., Dang PM, Puterka G, Shatters RG, Jr, McKenzie CL, Sinisterra XH. 2005.  5th Instar Glassy-winged Sharpshooter Homalodisca coagulata, (Hemiptera: Cicadellidae) Accession No. DN195890-DN203579, 3,921 mRNA seqs. NCBI. (Public).

                                                   

                                                  10.     Hunter W.B., Mizell RF, III, Tipping C, Dang PM, Hunnicutt LE. 2005.  Adult sharpshooter leafhopper Oncometopia nigricans, (Hemiptera: Cicadellidae), cDNA clones, Acc.No. DR755012-DR759538. 4,527 mRNA seqs. NCBI. (Public Database).

                                                   

                                                  11.     Weathersbee, AA, III, Hunter, W.B., Panchal, TD, Dang, PM. 2005. Lysiphlebus testaceipes, (Hymenoptera: Braconidae), Acc. No. DQ062217- DQ062237 and AY863030- AY961584. Set of 123 putative full cDNA sequence identities. (Public).

                                                  12.     Hunter W.B., Dang P.M., Lee L. 2005.  Adult alate Aphis gossypii, (Hemiptera: Aphididae), Acc. No. DR389179-DR397419. 8,241 mRNA seqs. NCBI. (Public).

                                                   

                                                   

                                                  13.     Hunter W.B., Smith M. 2005. 5th-instar Anoplophora glabripennis larva (Coleoptera: Cerambicidae) cDNA clones, Acc. No. DR108748-DR109303, Set of 556 mRNA seqs. Plus two full-length cDNA, DQ067276 & DQ067275.  NCBI. (Public Database).

                                                   

                                                  14.     Hunter W.B., Dang, PM., McKenzie, C.L. 2005 Diaphorina citri Kuwayama (Hemiptera: Psyllidae) Diaphorina citri, Acc. No. DN465721-DN470410, 4,686 mRNA sequences. NCBI. (Public Database).

                                                   

                                                  15.     Hunter W.B, McKenzie C.L., Shatters RG., Weathersbee AA., Hall D.G. 2005. Gene expression in field collected Asian citrus psylla, Diaphorina citri Kuwayama (Hemiptera: Psyllidae) 1,220 EST’s, Acc. No. DN202325- DN201110. NCBI.  (Public Database).

                                                   

                                                  16.     Hunter, W.B.. 2005. Nasonia, Produced three cDNA expression libraries, (Sequencing done by TIGR, 17,000 sequences, TIGR database).

                                                   

                                                  17.     Hunter W.B., and Dang, P.M. 2005. Gene expression in filed collected Asian citrus psyllid, Diaphorina citri, (Hemiptera: Psyllidae).  National Center for Biotechnology Information, NCBI (Public Database).

                                                   

                                                  18.     Hunter W.B.., Dang P.M, Cabrera I. Powell CA. 2005. Gene expression in field collected Citrus Root Weevil, Diaprepes abbreviatus L. (DRW) (Coleoptera: Curculionidae). Acc. No. DN199437-DN201109. 1,674 mRNA seqs. NCBI. (Public Database).

                                                   

                                                  19.     Hunter, W.B., Hunnicutt, L.E., Hall, D.G.  2006. Ribosomal proteins of the Asian citrus psyllid, Diaphorina citri. 88 Proteins, Acc. No.  DQ765535-DQ673441. National Center for Biotechnology Information, NCBI.  (Public Database).

                                                   

                                                  20.     Hunter, W.B., Hunnicutt, L.E., Wistrom, C.M., Purcell, A.H. 2006.  Proteins expressed in the Blue-green sharpshooter, Graphocephala atropunctata (Hemiptera: Cicadellidae). 44 Proteins, Acc. No. DQ445499-DQ445542. NCBI (Public Database).

                                                   

                                                  21.     Weathersbee, A.A., Panchal, T.D., Hunter, W.B., and Dang, P.M. 2006.  Expressed Genes from Lysiphlebus testaceipes (Hymenoptera: Braconidae).  7,762 mRNA seqs, Acc. nos.: EH009595-EH017357.  NCBI. (Public Database).

                                                   

                                                  22.     Hunter, W.B., Backus, E.A., Hunnicutt, L.E., Habibi, J., Dang, P.M. 2006.  Salivary gland gene expression in glassy-winged sharpshooter, Homalodisca vitripennis (syn. H. coagulata) (Hemiptera: Cicadellidae), Published 3,933 seqs. Acc. No. EG372472-EG368539. NCBI.

                                                  23.     Hunter, W.B.., Backus, E.A., Hunnicutt, L.E., Habibi, J., Dang, P.M. 2006. Midgut gene expression in glassy-winged sharpshooter, Homalodisca vitripennis (syn. H. coagulata) (Hemiptera: Cicadellidae), Published 3,933 seqs. Accession no. EG368538-EG365118.  NCBI. (Public Database).

                                                  24.     Hunter, W.B., Hunnicutt, L.E. 2007. Dataset of 162 Proteins expressed in the pink hibiscus mealybug, Maconellicoccus hirsutus (Hemiptera: Pseudococcidae).  Accession No. EF070444-EF070605. NCBI. (Public Database).

                                                  25.     Hunter, W.B., Dang, P.M., Nguyen, R. 2007.  Genes expressed in field-caught pink hibiscus mealybugs, Maconellicoccus hirsutus (Green) (Hemiptera: Pseudococcidae).  7,669 mRNA seqs, Acc. No. EH211307-EH218975. NCBI. (Public Database).

                                                  26.     Hunter, W.B., Hunnicutt, L.E., Wistrom, C.M., Purcell, A.H. 2007.  Gene expression in adult blue-green sharpshooters, Graphocephala atropunctata (Signoret) (Hemiptera: Cicadellidae).  6,481 mRNA seqs. Acc. No. EH655849-EH662332. NCBI. (Public).

                                                  27.     Valles, S.M., Strong, C.A., Dang, P.M., Hunter, W.B.., Pereira, R.M., Oi, D.H., Williams, D.F.  2007.  1,054 unique sequences from Solenopsis invicta, were deposited into the GenBank database under accession numbers EH412746 through EH413799.

                                                  28.     Hunter, W.B., Hall, DG. 2008. Midgut gene expression in Asian citrus psyllid, Diaphorina citri (Hemiptera: Psyllidae) 6,200 EST’s, FK254041- FK260232. NCBI.

                                                  29.     Hunter, W.B., Hall, D.G. 2008. Testes gene expression in Asian citrus psyllid, Diaphorina citri (Hemiptera: Psyllidae). GD264847-GD269020. NCBI.

                                                  30.     Hunter, W.B., Reese, J., Shatters, RG, Hall, DG, Leng, N., et al. 2011. Asian Citrus Psyllid Genome. DIACI_1.0 draft genome database, http://www.psyllid.org.

                                                   

                                                  31.     Hunter, W.B., Reese, J., Shatters, RG, Hall, DG., Leng, N. 2011. Asian citrus psyllid transcriptome.  DIACI_Transcriptome_0.9.  http://www.psyllid.org .

                                                   

                                                  Sequencing Links:

                                                  Asian Citrus Psyllid ESTs http://www.psyllid.org

                                                  D. C. S. G. Oliveira, W. B. Hunter, J. Ng,C. A. Desjardins, P. M. Dang, and J. H. Werren.  Data mining cDNAs reveals three new single stranded RNA viruses in Nasonia (Hymenoptera: Pteromalidae). Insect Molecular Biology, 2010, 19 (Suppl 1), 99-107.

                                                  Hunter, Wayne B, Shelby, Kent S., Purcell, Alexander H., and Laura Hunnicut. Protein Identities - GRAPHOCEPHALA ATROPUNCTATA Expressed sequence tags: Expanding Leafhopper Vector Biology. 2009, FLSC-73-01-11.3d, 89-107.

                                                  W.B. Hunter,S.E. Dowd, D.S. Katsar, R.G. Shatters Jr., C.L. McKenzie and D.G. Hall.  Psyllid Biology:  Expressed Genes in Adult Asian Citrus Psyllids, Diaphorina citri Kuwayama.  The Open Entomology Journal, 2009, 3, 18-29.

                                                  Dr. Hunter's Publication List (2002-2009)

                                                  Dr. Hunter's Publications (1995 -2002):

                                                  Hunter, W.B., Sinisterra, X.H., McKenzie, C.L., and Shatters, Jr., R.G. 2002. Iridovirus infection and vertical transmission in citrus aphids. Proceedings of the Florida Horticultural Society 114:70-72.

                                                  McKenzie, CL., Lapointe, SL., Hunter, WB., and GJ. Puterka. 2002. Efficacy of Surround for control of Asian citrus psyllid on citrus. Arthropod Management Tests 27: (Report).

                                                  McKenzie, C.L., W.B. Hunter, S.L. Lapointe, and P.M. Dang. 2002. Iridovirus: potential entomopathogenic biocontrol agent for whitefly. Silverleaf Whitefly National Research, Action, and Technology Transfer Plan: Fourth Annual Review of the Second 5-Year Plan and Final Report for 1992-2002. USDA-ARS 2002-01: 162.

                                                  McKenzie, CL., Hunter, WB., Lapointe, SL., and Dang, P. 2002. Iridovirus infection and topical application to whitefly. Proceedings of the Florida State Horticultural Society 114:64-67.

                                                  Hunter, W.B., and Polston, J.E. 2001. Development of a continuous whitefly cell line [Homoptera: Aleyrodidae: Bemisia tabaci (Gennadius)] for the study of Begomovirus.Journal of Invertebrate Pathology 77: 33-36.

                                                  Funk, C J., Hunter, W.B., and Achor, DS. 2001. Replication of insect iridescent virus 6 in a whitefly cell line. Journal of Invertebrate Pathology 77: 144-146.

                                                  Mayer, Richard T., Wayne B. Hunter, Stephen L. Lapointe, C. L. McKenzie, R. Shatters, J. Shapiro, Hamed Doostdar and A. A. Weathersbee III. 2001. Diaprepes Root Weevil: Recent Advances at the U.S. Horticultural Research Laboratory, Fort Pierce. Citrus & Vegetable Magazine, Part 2, Vol. 65, No. 12, August 2001, pp. 8-13.

                                                  Mayer, Richard T., Wayne B. Hunter, Stephen L. Lapointe, C. L. McKenzie, R. Shatters, J. Shapiro, Hamed Doostdar and A. A. Weathersbee III. 2001. Diaprepes Root Weevil: Recent Advances at the U.S. Horticultural Research Laboratory, Fort Pierce. Citrus & Vegetable Magazine. Part 1, Vol. 65, No. 11, July 2001, pp. 9-12.

                                                  Hunter, W.B., C.P. Patte, X.H. Sinisterra, D.S. Achor, C.J. Funk and J.E. Polston. 2001. Discovering new insect viruses: Whitefly iridovirus (Homoptera: Aleyrodidae: Bemisia tabaci). Journal of Invertebrate Pathology 78: 220-225.

                                                  Lapointe, S. L., W. B. Hunter, and C. J. Funk(2000). Infection of the Diaprepes root weevil, Diaprepes abbreviatus (Coleoptera: Curculionidae), by an Iridovirus.

                                                  Hunter, W.B.and H.T. Hsu. 1999. Synthetic diets and piercing-sucking insect research. Intl. J. Ecol. Environ. Science 25: 259-266.

                                                  Tang, Y.Q., S.L. Lapointe, L.C. Brown, and W. B. Hunter. 1999. Effects of host plant and temperature on the biology of Toxoptera citricida (Homoptera: Aphididae). Environ. Entomol. 28(5): 895-900.

                                                  Hunter, W. B., E. Hiebert, S. E. Webb, J. H. Tsai & J. E. Polston. 1997. Location of geminivirus in the whitefly, Bemisia tabaci (Homoptera: Aleyrodidae). Plant Dis. 82: 1147-1151.

                                                  Hunter, W. B., E. Hiebert, S. Webb, J. E. Polston, and J. H. Tsai. 1996 Precibarial and Cibarial Chemosensilla in the Whitefly, Bemisia Tabaci(Gennadius), (Homoptera: Aleyrodidae) Int. J Insect Morphol. and Embryol. 25(3): 295-304.

                                                  Hunter, W. B.& H. T. Hsu. 1996. Formulation of an insect medium for thrips monolayer cell cultures (Thysanoptera: Thripidae: Frankliniella occidentalis). J. Invert. Pathol. 67:125-128.

                                                  Hunter, W. B.& H. T. Hsu. 1996. Inoculation of thrips cell mono-layers with a tospovirus. pp. 359-363. In International Symposium on Tospoviruses and Thrips of Floral and Vegetable Crops. George Kuo (ed). Acta Horticulturae, No. 431. ISHS. Asian Vegetable Research & Development Center, AVRDC.

                                                  Hunter, W. B.& H. T. Hsu. 1995. Establishing Thrips Cell Cultures to Study Tospoviruses.pp. 163-166. In Thrips Biology and Management, Bruce L. Parker, Margaret Skinner, and Trevor Lewis (eds). Plenum Press, New York.

                                                  Hunter, W. B., H. T. Hsu & R. Lawson. 1995. A novel method for acquisition of Tospoviruses by thrips. Phytopathology 85(4): 480-483. (Public Database).

                                                  Dr. Hunter's Project List

                                                  Published EST Gene Sequences:

                                                  Hunter, W.B, Hunnicutt, L.E. 2007. Dataset of 162 Proteins expressed in the pink hibiscus mealybug, Maconellicoccus hirsutus(Hemiptera: Pseudococcidae). Accession no. EF070444-EF070605. National Center for Biotechnology Information, NCBI. (Public Database).

                                                  Hunter, W.B., Dang, P.M., Nguyen, R. 2007. Genes expressed in field-caught pink hibiscus mealybugs, Maconellicoccus hirsutusNCBI.(Public Information, Biotechnology for Center National EH211307-EH218975. nos.: Acc. sequences, mRNA 7,669 Pseudococcidae). (Hemiptera: (Green)>

                                                  Hunter, W.B., Hunnicutt, L.E., Wistrom, C.M., Purcell, A.H. 2007. Gene expression in adult blue-green sharpshooters, Graphocephala atropunctata(Signoret) (Hemiptera: Cicadellidae)6,481 mRNA sequences. Acc. nos.: EH655849-EH662332. National Center for Biotechnology Information, NCBI. (Public Database).

                                                  Mozoruk, J, Hunnicutt, LE, Cave, RD, Hunter, W.B. , Bausher, MG. 2006. Profiling transcriptional changes in Citrus sinensis(L.) Osbeck challenged by herbivory from the xylem-feeding leafhopper Homalodisca coagulata(Say) by cDNA macroarray analysis. Plant Science 170:1068-1080.

                                                  Hunter,W.B.,Hunnicutt,L.E., Hall, D.G. 2006. Ribosomal proteins of the Asian citrus psyllid, Diaphorina citri. 88 Proteins, Acc. nos: DQ765535-DQ673441. National Center for Biotechnology Information, NCBI. http://www4.ncbi.nlm.nih.gov/. (Public Database).

                                                  Hunter, W.B., Hunnicutt, L.E., Wistrom, C.M., Purcell, A.H. 2006. Proteins expressed in the Blue-green sharpshooter, Graphocephala atropunctata(Hemiptera: Cicadellidae). 44 Proteins, Acc. nos.: DQ445499-DQ445542. National Center for Biotechnology Information, NCBI. (Public Database).

                                                  Weathersbee, A.A., Panchal,T.D., Hunter,W.B.and Dang,P.M. 2006. Expressed Genes from Lysiphlebus testaceipes(Hymenoptera: Braconidae). 7,762 mRNA sequences, Acc. nos.: EH009595-EH017357. National Center for Biotechnology Information, NCBI. (Public Database).

                                                  Hunter W.B.,Dang PM, Puterka G, Shatters RG, Jr, McKenzie CL, Sinisterra XH. 2005. 5th Instar Glassy-winged Sharpshooter Homalodisca coagulata, (Hemiptera: Cicadellidae) Accession numbers- DN195890-DN203579, 3,921 mRNA sequences. National Center for Biotechnology Information, NCBI. http://www.ncbi.nlm.nih.gov/

                                                  Hunter W.B.,Mizell RF, III, Tipping C, Dang PM, Hunnicutt LE. 2005. Adult sharpshooter leafhopper Oncometopia nigricans, (Hemiptera: Cicadellidae), cDNA clones, Acc.Nos.-DR755012-DR759538. 4,527 mRNA sequences. NCBI. http://www.ncbi.nlm.nih.gov/

                                                  Weathersbee, AA, III, Hunter,W.B., Panchal, TD, Dang, PM. 2005. Lysiphlebus testaceipes,(Hymenoptera: Braconidae), Acc. Nos.- DQ062217- DQ062237 and AY863030-AY961584. Set of 123 putative full cDNA sequences with identities. http://www.ncbi.nlm.nih.gov/

                                                  Hunter W.B., Dang PM, Lee L. 2005. Adult alate Aphis gossypii, (Hemiptera: Aphididae), Acc. No.-DR389179-DR397419.   8,241 mRNA sequences. NCBI.   http://www.ncbi.nlm.nih.gov/  

                                                  Hunter W.B., Smith M. 2005. 5th-instar Anoplophora glabripennislarva (Coleoptera: Cerambicidae) cDNA clones, Acc. Nos.-DR108748-DR109303, Set of 556 mRNA sequences. Plus two full-length cDNA, DQ067276 & DQ067275. NCBI. http://www.ncbi.nlm.nih.gov/  

                                                  Hunter W.B. , Dang, PM, McKenzie, CL. 2005 Diaphorina citriKuwayama (Hemiptera: Psyllidae) Diaphorina citri, Acc. nos.- DN465721-DN470410, 4,686 mRNA sequences http://www.ncbi.nlm.nih.gov/

                                                  Hunter W.B., McKenzie CL, Shatters RG. Weathersbee AA. Hall DG. Gene expression in field collected Asian citrus psylla, Diaphorina citriKuwayama (Hemiptera: Psyllidae) 1,220 EST’s, Acc. Nos. DN202325- DN201110. NCBI. http://www.ncbi.nlm.nih.gov/  

                                                  Werren, J, Hunter, W., Dang, P. 2005. Nasonia, Produced cDNA expression libraries, (Sequencing done by TIGR, 17,000 sequences,TIGR database).

                                                  Hunter W.B. and Dang, PM. 2005. Gene expression in filed collected Asian citrus psyllid, Diaphorina citri, (Hemiptera: Psyllidae). National Center for Biotechnology Information, NCBI.   http://www.ncbi.nlm.nih.gov/

                                                  Hunter WB., Dang PM, Cabrera I. Powell CA. 2005. Gene expression in field collected Citrus Root Weevil, Diaprepes abbreviatusL. (DRW) (Coleoptera: Curculionidae). Acc. Nos.-DN199437-DN201109. 1,674 mRNA sequences. NCBI. (Public Database).

                                                  http://www.ncbi.nlm.nih.gov/

                                                  Hunter, WB.Dang,P.M., Chaparro,J.X., Lu,J., Huang,H. and Leong,S. 2004. Genes expressed in Vitis shuttleworthii. Grapes. Accession no. CN603662-CN605482, CV092198- CV100862, CF568781-CF568998.   NationalCenterfor Biotechnology Information, NCBI. http://www.ncbi.nlm.nih.gov/   (Public Database).

                                                  Hunter, W.B.2003. Published 4,320 electronic pages: Adult Alate Brown Citrus Aphid Toxoptera citricidacDNA clones.   U.S.Horticultural Research Laboratory, 2001 South Rock Rd., Fort Pierce, FL34945, USA.   Accession no. CB832665-CD452353. NationalCenterfor Biotechnology Information, NCBI. http://www.ncbi.nlm.nih.gov/  (Public Database).

                                                  Hunter, WB.2003. Published 427 electronic pages. Data Set: Glassy-winged Sharpshooter, Expressed Sequence Tags, ESTs, from Adult Homalodisca coagulata(Say) (Hemiptera: Cicadellidae). GenBank Accession Numbers CF194966 through CF195393.   NationalCenterfor Biotechnology Information, NCBI. http://www.ncbi.nlm.nih.gov/  (Public Database).

                                                  Hunter, W.B.and Dang, P.M. 2003. Published 970 electronic pages: Gene expression in Acyrthosiphon pisum(Hemiptera: Aphididae).   U.S.Horticultural Research Laboratory, 2001 South Rock Rd., Fort Pierce, FL34945, USA.   Accession no. CF587442-CF588411.   NationalCenterfor Biotechnology Information, NCBI. http://www.ncbi.nlm.nih.gov/  (Public Database).

                                                  Bausher, M., McKendree, W., Dang, P., Chaparro, J., Shatters, R., Hunter, W.B., Niedz, R 2002. Published 2,306 electronic pages of “EST high quality sequences from a Citrus sinensis(sweet orange) whole seedling cDNA library” to NCBI EST database.   http://www.ncbi.nlm.nih.gov/ (Public Database).

                                                  Datasets Submitted NCBI:

                                                  Hunter, W.B., Backus, E.A., Hunnicutt, L.E., Habibi, J., Dang, P.M. 2006. Salivary gland gene expression in glassy-winged sharpshooter, Homalodisca vitripennis (syn. H. coagulata) (Hemiptera: Cicadellidae), Published 3,933 sequences. Accession nos.: EG372472-EG368539. National Center for Biotechnology Information, NCBI. (Public Database).

                                                  Hunter, W.B., Backus, E.A., Hunnicutt, L.E., Habibi, J., Dang, P.M. 2006. Midgut gene expression in glassy-winged sharpshooter, Homalodisca vitripennis (syn. H. coagulata) (Hemiptera: Cicadellidae), Published 3,933 sequences. Accession # EG368538-EG365118. National Center for Biotechnology Information, NCBI. (Public Database).

                                                  Desjardins, C.A., Oliveira, D.C.S.G., Edwards, R.M., Dang, P.M., Lee, D., Colbourne, J.K., Tettelin, H., Hunter, W.B., Werren, J.H. 2007. Nasonia expression libraries from larvae, pupae, and adults. 37,360 sequences published. Acc. no.: ES6512267-ES613911. National Center for Biotechnology Information, NCBI. (Public Database).


                                                  Last Modified: 2/19/2013