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United States Department of Agriculture

Agricultural Research Service

Related Topics

Charles Lee
Bioproduct Chemistry and Engineering Research
Research Chemist

Phone: (510) 559-5858
Fax: (510) 559-5818
Room 3113
USDA, ARS, PWA, WRRC-BCE
800 BUCHANAN STREET
ALBANY, CA, 94710

Projects
Biorefining Processes
Appropriated (D)
  Accession Number: 418775
NANOSTRUCTURED ENZYME ASSEMBLIES FOR LIGNOCELLULOSIC BIOMASS BREAKDOWN FOR BIOPRODUCT AND BIOENERGY APPLICATIONS
Reimbursable (R)
  Accession Number: 423540

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Directed evolution of GH43 ß-xylosidase XylBH43 thermal stability and L186 saturation Reprint Icon - (Peer Reviewed Journal)
Singh, S.K., Heng, C., Braker, J.D., Chan, V.J., Lee, C.C., Jordan, D.B., Yuan, L., Wagschal, K.C. 2013. Directed evolution of GH43 ß-xylosidase XylBH43 thermal stability and L186 saturation. Journal of Industrial Microbiology and Biotechnology. 41(3):489-498. DOI: 10.1007/s10295-013-1377-0.
Activation of a GH43 ß-xylosidase by divalent metal cations: Slow binding of divalent metal and high substrate specificity - (Peer Reviewed Journal)
Jordan, D.B., Lee, C.C., Wagschal, K., Braker, J.D. 2013. Activation of a GH43 ß-xylosidase by divalent metal cations: Slow binding of divalent metal and high substrate specificity. Archives of Biochemistry and Biophysics. 533:79-87.
Divalent metal activation of a GH43 ß-xylosidase - (Peer Reviewed Journal)
Lee, C.C., Braker, J.D., Grigorescu, A.A., Wagschal, K.C., Jordan, D.B. 2013. Divalent metal activation of a GH43 ß-xylosidase. Enzyme and Microbial Technology. 52(2):84-90.
Selenium speciation analysis of Misgurnus anguillicaudatus selenoprotein by HPLC-ICP-MS and HPLC-ESI-MS/MS - (Peer Reviewed Journal)
Gong, L., Xu, Q., Lee, C.C., Zhang, H. 2012. Selenium speciation analysis of Misgurnus anguillicaudatus selenoprotein by HPLC-ICP-MS and HPLC-ESI-MS/MS. European Food Research and Technology. 235 (1) 169-176.
Isolation and characterization of a novel GH67 a-glucuronidase from a mixed culture - (Peer Reviewed Journal)
Lee, C.C., Wagschal, K.C., Ruiping, L., Robertson, G.H., Kibblewhite, R.E., Orts, W.J. 2012. Isolation and characterization of a novel GH67 a-glucuronidase from a mixed culture. Journal of Industrial Microbiology and Biotechnology. 39:1245-1251. DOI:10.1007/s10295-012-1128-7.
Development of enzymes and enzyme systems by genetic engineering to convert biomass to sugars - (Abstract Only)
Lee, C.C., Wagschal, K.C. 2013. Development of enzymes and enzyme systems by genetic engineering to convert biomass to sugars. [Abstract}. California American Chemical Society Section Meeting, April 25, 2012, Albany CA.
Nanostructured enzyme assemblies for lignocellulosic biomass breakdown for bioproduct and bioenergy applications - (Abstract Only) - (25-Jan-12)
Microplate-based active/inactive 1 screen for biomass degrading enzyme library purification and gene discovery - (Peer Reviewed Journal)
Wagschal, K.C., Lee, C.C. 2012. Microplate-based active/inactive 1 screen for biomass degrading enzyme library purification and gene discovery. Analytical Biochemistry. 89: 83-85.
Plant cell walls to ethanol. - (Peer Reviewed Journal)
Jordan, D.B., Bowman, M.J., Braker, J.D., Dien, B.S., Hector, R.E., Lee, C.C., Mertens, J.A., Wagschal, K.C. 2012. Plant cell walls to ethanol. Biochemical Journal. 442:247-252.
Isolation of alpha-glucuronidase enzyme from a rumen metagenomic library - (Peer Reviewed Journal)
Lee, C.C., Kibblewhite, R.E., Wagschal, K.C., Li, R., Orts, W.J. 2012. Isolation of alpha-glucuronidase enzyme from a rumen metagenomic library. Protein Journal. 31(3):206-211.
Hilic Separation Mechanisms of Tetracyclines on Amino Bonded Silica Column - (Peer Reviewed Journal)
Li, R., Zhang, Y., Lee, C.C., Liu, L., Huang, Y. 2011. HILIC separation mechanisms of tetracyclines on amino bonded silica column. Journal of Separation Science. 34(13):1508-1516.
A high-throughput protein expression system in Pichia pastoris using a newly developed episomal vector - (Peer Reviewed Journal)
Sasagawa, T., Moriya, S., Lee, C.C., Kitamoto, K., Arioka, M. 2011. A high-throughput protein expression system in Pichia pastoris using a newly developed episomal vector. Plasmid Journal. 65(1):65-69.
Screening and characterization of hemicellulose-degrading enzymes - (Abstract Only)
Lee, C.C., Wagschal, K.C., Orts, W.J. Screening and characterization of hemicellulose-degrading enzymes. 2010 SIM Annual Meeting and Exhibition; Abstract S160b: p101.
Screening assays for biomass-degrading enzymes - (Peer Reviewed Journal)
Lee, C.C. 2010. Screening assays for biomass-degrading enzymes. Biofuels. 1(4):575-588.
Cloning and characterization of alpha-glucuronidase enzymes from mixed cultures - (Abstract Only)
Lee, C.C., Kibblewhite, R.E., Wagschal, K.C., Orts, W.J. 2010. Cloning and characterization of alpha-glucuronidase enzymes from mixed cultures. Meeting Abstract. 85.
Development and validation of a hydrophilic interaction liquid chromatographic method for determination of aromatic amines in environmental water - (Peer Reviewed Journal)
Li, R., Zhang, Y., Lee, C.C., Lu, R., Huang, Y. Development and validation of a hydrophilic interaction liquid chromatographic method for determination of aromatic amines in environmental water. Journal of Chromatography, 2010. A 1217(11), pp 1799-1805.
Cloning and characterization of alpha-glucuronidase enzyme - (Abstract Only)
Lee, C.C., Kibblewhite, R.E., Wagschal, K.C., Wong, D., Orts, W.J. 2010. Cloning and characterization of alpha-glucuronidase enzyme. Meeting Abstract.
Cloning and characterization of alpha-glucuronidase enzyme - (Abstract Only)
Lee, C.C., Kibblewhite, R.E., Wagschal, K.C., Wong, D., Orts, W.J. 2010. Cloning and characterization of alpha-glucuronidase enzyme. Meeting Abstract.
Chromosomal integration of recombinant alpha-amylase and glucoamylase genes in saccharomyces cerevisiae for starch conversion - (Peer Reviewed Journal)
Wong, D., Batt Throne, S.B., Robertson, G.H., Lee, C.C., Wagschal, K.C. 2010. Chromosomal integration of recombinant alpha-amylase and glucoamylase genes in saccharomyces cerevisiae for starch conversion. Industrial Biotechnology. 6:112-118.
Cloning and characterization of an alpha-glucuronidase from a mixed microbial population - (Peer Reviewed Journal)
Lee, C.C., Kibblewhite, R.E., Wagschal, K.C., Robertson, G.H., Orts, W.J. 2009. Cloning and characterization of an alpha-glucuronidase from a mixed microbial population. Enzyme and Microbial Technology. 155(1-3):314-320.
Molecular cloning and characterization of multidomain xylanase from manure library - (Peer Reviewed Journal)
Li, R., Kibblewhite, R.E., Orts, W.J., Lee, C.C. 2009. Molecular cloning and characterization of multidomain xylanase from manure library. World Journal of Microbiology and Biotechnology. doi:10:1007/s11274-009-011106
Engineer Sccharomyces cerevisiae for consolidated bioprocessing - (Abstract Only)
Wong, D., Batt Throne, S.B., Wan, D., Lee, C.C., Wagschal, K.C. 2009. Engineer Sccharomyces cerevisiae for consolidated bioprocessing. Meeting Abstract.
Cloning biomass-degrading enzymes from environmental sources - (Abstract Only)
Lee, C.C., Wong, D., Wagschal, K.C., Orts, W.J. 2009. Cloning biomass-degrading enzymes from environmental sources. Meeting Abstract.
Purification and characterization of a glycoside hydrolase family 43 Beta-xylosidase from Geobacillus thermoleovorans IT-08 - (Peer Reviewed Journal)
Wagschal, K.C., Heng, C., Lee, C.C., Robertson, G.H., Orts, W.J., Wong, D. 2008. Purification and characterization of a glycoside hydrolase family 43 Beta-xylosidase from Geobacillus thermoleovorans IT-08. Applied Biochemistry and Biotechnology. 155(1-3):1-10.
Biochemical characterization of a novel dual-function a-L-arabinofuranosidase/b-xylosidase isolated from a compost starter mixture - (Peer Reviewed Journal)
Wagschal, K.C., Heng, C., Lee, C.C., Wong, D. 2009. Biochemical characterization of a novel dual-function a-L-arabinofuranosidase/b-xylosidase isolated from a compost starter mixture. Applied Microbiology and Biotechnology. 81:855-863.
Metagenomic gene discovery and enzyme engineering for biomass conversion - (Abstract Only)
Wong, D., Lee, C.C., Wagschal, K.C., Smith, M.R., Robertson, G.H., Orts, W.J. Metagenomic gene discovery and enzyme engineering for biomass conversion. American Chemical Society Western Regional Meeting. American Chemical Society Symposium: New Developments in Biofuels Research. Abstract 204.
Gene discovery and engineering for biomass degradation - (Proceedings)
Lee, C.C., Wagschal, K.C., Wong, D. 2008. Gene discovery and engineering for biomass degradation. United States-Japan Cooperative Program in Natural Resources. 2008. 50-51.
Cloning of Bacillus licheniformis xylanase gene and characterization of recombinant enzyme - (Peer Reviewed Journal)
Lee, C.C., Accinelli, R., Smith, M.R., Wagschal, K.C., Orts, W.J., Wong, D. 2008. Cloning of Bacillus licheniformis xylanase gene and characterization of recombinant enzyme. Current Microbiology. 57:301-305.
Biofuels Group NSF DUSEL Project - (Abstract Only)
Bang, S., Sani, R., Bleakly, B., Bischoff, K.M., Hughes, S.R., Wagschal, K.C., Wong, D., Lee, C.C., Jordan, D.B., Pokkuluri, R., Schiffer, M. 2008. Biofuels Group NSF DUSEL Project [abstract]. Homestake DUSEL Spring Workshop. Talk 10. p. 2.
Cloning, Expression and Characterization of a Glycoside Hydrolase Family 39 Xylosidase from Bacillus Halodurans C-125 - (Peer Reviewed Journal)
Wagschal, K.C., Franquivillanueva, D.M., Lee, C.C., Robertson, G.H., Wong, D. Cloning, Expression and Characterization of a Glycoside Hydrolase Family 39 Xylosidase from Bacillus Halodurans C-125. Applied Biochemistry and Biotechnology.
Cloning and characterization of xylanase gene from hot spring microorganism - (Abstract Only)
Lee, C.C., Accinelli, R., Smith, M.R., Wagschal, K.C., Orts, W.J., Wong, D. Cloning and characterization of xylanase gene from hot spring microorganism. Meeting Abstract #307.
Developing enzyme systems for biomass deconstruction - (Abstract Only)
Wong, D., Lee, C.C., Wagschal, K.C., Smith, M.R., Robertson, G.H., Orts, W.J. 2007. Developing enzyme systems for biomass deconstruction. Meeting Abstract. Paper No. 217
Satb1 Packages Densely Looped, Transcriptionally Active Chromatin for Coordinated Expression of Cytokine Genes - (Peer Reviewed Journal)
Cai, S., Lee, C.C., Kohwi-Shigematsu, T. 2006. Satb1 packages densely looped, transcriptionally active chromatin for coordinated expression of cytokine genes. Nature Genetics. (38):1278-1288
Expression and Secretion of Clostridium Cellulovorans Xyna in Saccharomyces Cerevisiae - (Abstract Only)
Batt, S.B., Wong, D., Wagschal, K.C., Lee, C.C. 2006. Expression and secretion of clostridium cellulovorans xyna in saccharomyces cerevisiae. Division of Biological Chemistry, Enzymes Program. Abstract 220.
Genetic and Biochemical Characterization of a Novel Bacterial A-L-Arabinofuranosidase - (Peer Reviewed Journal)
Wagschal, K.C., Franqui Espiet, D., Lee, C.C., Accinelli, R., Robertson, G.H., Wong, D. 2007. Genetic and biochemical characterization of a novel bacterial a-l-arabinofuranosidase. Enzyme and Microbial Technology. (40):747-753.
Evolutionary Enzyme Design for Improved Biorefining of Crops and Residues - (Abstract Only)
Wong, D., Lee, C.C., Wagschal, K.C., Smith, M.R., Robertson, G.H., Orts, W.J. 2006. Evolutionary enzyme design for improved biorefining of crops and residues. Technical Program (abstract). P8.
Synergistic Action of Barley Alpha-Amylase and Lentinula Edodes Glucoamylase on Raw Starch Hydrolysis - (Abstract Only)
Wong, D., Robertson, G.H., Lee, C.C., Wagschal, K.C. 2006. Synergistic action of barley alpha-amylase and lentinula edodes glucoamylase on raw starch hydrolysis. Approved 10/27/2005. American Society of Biochemistry & Molecular Biology, San Francisco, CA. April 1-4, 2006, Program 73.16, vol A27.
Cloning and Characterization of Cold-Active Xylanase Enzymes - (Abstract Only)
Lee, C.C., Accinelli, R., Wagschal, K.C., Robertson, G.H., Wong, D. 2006. Cloning and characterization of cold-active xylanase enzymes. 28th Symposium on Biotechnology for Fuels and Chemicals. Paper No. 1A-41.
Cloning and Characterization of a Cold Active Xylanase Enzyme - (Peer Reviewed Journal)
Lee, C.C., Accinelli, R., Wagschal, K.C., Robertson, G.H., Wong, D. 2006. Cloning and characterization of a cold active xylanase enzyme. Extremophiles. 10:295-300.
Native Or Raw Starch Digestion - (Review Article)
Robertson, G.H., Wong, D., Lee, C.C., Wagschal, K.C., Smith, M.R., Orts, W.J. 2006. Native or raw starch digestion: A Key step in energy efficient biorefining of grain. Journal of Agricultural and Food Chemistry, 54:353-65.
Isolation and Characterization of a Psychrophilic Xylanase Enzyme from Flavobacterium Sp. - (Peer Reviewed Journal)
Lee, C.C., Smith, M.R., Accinelli, R., Williams, T.G., Wagschal, K.C., Wong, D., Robertson, G.H. 2006. Isolation and characterization of a psychrophilic xylanase enzyme from flavobacterium sp. Current Microbiology, 52(2):112-116.
Characterization of Active Lentinula Edodes Glucoamylase Expressed and Secreted by Saccharomyces Cerevisiae - (Peer Reviewed Journal)
Wong, D., Batt, S.B., Lee, C.C., Wagschal, K.C., Robertson, G.H. 2005. Characterization of active lentinula edodes glucoamylase expressed and secreted by saccharomyces cerevisiae. Protein Journal. vol 24, pp 455-463.
Characterization and Directed Evolution of Hemicellulotic Enzymes Using Natural Xylooligoaccharide Substrates - (Abstract Only)
Wagschal, K.C., Franqui Espiet, D., Lee, C.C., Robertson, G.H., Wong, D.W.S. 2005. Characterization of hemicellulotic degrading enzymes using natural substrates. Symposium on Biotechnology for Fuels and Chemicals, Denver, CO. Paper No. 1B-65. Abstract A10, Enzyme Engineering XVIII.
Enzyme-Coupled Assay for Beta-Xylosidase Hydrolysis of Natural Substrates - (Peer Reviewed Journal)
Wagschal, K.C., Franqui Espiet, D., Lee, C.C., Robertson, G.H., Wong, D. 2005. Enzyme-coupled assay for beta-xylosidase hydrolysis of natural substrates. Applied and Environmental Microbiology. 71:5318-5323.
Evolved Enzymes for Cold Or Non-Cooking Hydrolysis of Starches to Fermentation Feedstock - (Abstract Only)
Robertson, G.H., Wong, D., Lee, C.C., Wagschal, K.C., Smith, M.R. 2005. Evolved enzymes for cold or non-cooking hydrolysis of starches to fermentation feedstock. In Proceedings of the North American Grain Congress, February 20-22, 2005, Reno, NV. 2005 CDROM.
Expression and Characterization of Active Lentinus Edodes Glucoamylase in Saccharomyces Cerevisiae - (Abstract Only)
Wong, D., Batt, S.B., Lee, C.C., Wagschal, K.C., Robertson, G.H. 2005. Expression and characterization of active. lentinus edodes glucoamylase in saccharomyces cerevisiae. Presentation 04:05. Paper No 11277.
Cloning and Characterization of Biomass Degrading Enzymes - (Abstract Only)
Lee, C.C., Accinelli, R., Batt, S.B., Williams, T.G., Wong, D., Wagschal, K.C., Robertson, G.H. 2005. Cloning and characterization of biomass degrading enzymes. Symposium on Biotechnology for Fuels and Chemicals, Denver, CO. Paper No. IB-41 (Abstract).
Directed Evolution of Barley Alpha-Amylase for Increased Activity - (Abstract Only)
Batt, S.B., Wong, D., Lee, C.C., Wagschal, K.C., Robertson, G.H. 2004. Directed evolution of barley alpha-amylase for increased activity. American Chemical Society Western Regional Meeting, Sacramento, CA. Paper No. 144 (Abstracts).
Heterogeneous Biocatalyic Liquefaction and Saccharificaation of Starch for Ethanol - (Abstract Only)
Robertson, G.H., Wong, D., Lee, C.C., Wagschal, K.C. 2004. Heterogeneous biocatalyic liquefaction and saccharificaation of starch for ethanol. Corn Utilization Conference Proceedings, June 6-9, 2004, Indianapolis, Indiana, Abstract.
In Vitro Evolution of High-Activity Barley Alpha-Amylase for Raw Starch Digestion - (Abstract Only)
Wong, D., Robertson, G.H., Batt, S.B., Lee, C.C. 2004. In vitro evolution of high-activity barley alpha-amylase for raw starch digestion. American Association of Cereal Chemists Meetings, September 19-22, 2004, San Diego, California. Abstract.
High-Activity Barley Alpha-Amylase by Directed Evolution - (Peer Reviewed Journal)
Wong, D., Batt, S.B., Lee, C.C., Robertson, G.H. 2004. High-activity barley alpha-amylase by directed evolution. Journal of Protein Chemistry, 23:53-460.
Rapid Selection of Alpha-Amylase Mutants in Yeast Libraries for Raw Starch Activity - (Abstract Only)
Wong, D., Batt, S.B., Lee, C.C., Robertson, G.H. Rapid selection of alpha-amylase mutants in yeast libraries for raw starch activity. 2003. Society of Biomolecular Screening Annual Conference, Portland, Oregon.
Engineering Apha-Amylase for Enhanced Activity by Molecular Evolution - (Abstract Only)
Wong, D., Batt, S.B., Lee, C.C., Robertson, G.H. Engineering apha-amylase for enhanced activity by molecular evolution. American Society of Biochemistry and Molecular Biology Annual Meeting, June 12-16, 2004, Boston, Massachusetts. Abstract No. B38.
An Episomal Expression Vector for Screening Mutant Gene Libraries in Pichia Pastoris - (Peer Reviewed Journal)
Lee, C.C., Williams, T.G., Wong, D., Robertson, G.H. 2004. An episomal expression vector for screening mutant gene libraries in pichia pastoris. Plasmid Journal. 54(1):80-85.
Direct Screening of Libraries of Yeast Clones for Alpha-Amylase Activity on Raw Starch - (Peer Reviewed Journal)
Wong, D., Batt, S.B., Lee, C.C., Robertson, G.H. 2004. Direct screening of libraries of yeast clones for alpha-amylase activity on raw starch. Protein and Peptide Letters. 10:459-468.
Cloning and Characterization of the Xyn11a Gene from Lentinula Edodes - (Peer Reviewed Journal)
Lee, C.C., Wong, D., Robertson, G.H. 2005. Cloning and characterization of the xyn11a gene from lentinula edodes. Protein Journal. 24(1):21-26.
ARS News Articles
Last Modified: 7/31/2014