Page Banner

United States Department of Agriculture

Agricultural Research Service

Bradley Jay Hernlem (Brad)
Foodborne Toxin Detection and Prevention
Chemical Engineer

Phone: (510) 559-5937
Fax: (510) 559-6429
USDA, ARS, PWA, WRRC-FTDP
800 BUCHANAN ST
ALBANY, CA, 94710

Projects
Technologies for Detecting and Determining the Bioavailability of Bacterial Toxins
Appropriated (D)
  Accession Number: 421099

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Quantitative analysis of Staphylococcus enterotoxin A by differential expression of IFN-gamma in splenocyte and CD4+ T-cells - (Peer Reviewed Journal)
Rasooly, R., Hernlem, B.J. 2014. Quantitative analysis of Staphylococcus enterotoxin A by differential expression of IFN-gamma in splenocyte and CD4+ T-cells. Sensors. 14:8869-8876 DOI:10.3390/s140508869.
Rapid detection of predation of Escherichia coli O157:H7 and sorting of bacterivorous tetrahymena by flow cytometry - (Peer Reviewed Journal)
Ravva, S.V., Hernlem, B.J., Sarreal, C.Z. 2014. Rapid Detection of predation of Escherichia coli O157:H7 and sorting of bacterivorous tetrahymena by flow cytometry. Frontiers in Cellular and Infection Microbiology. DOI:10.3389/fcimb.2014.000057.
Microwave heating inactivates Shiga Toxin (Stx2) in pH 7.4 buffer and in reconstituted fat-free Milk and adversely affects the nutritional value of cell culture medium - (Peer Reviewed Journal)
Rasooly, R., Hernlem, B.J., He, X., Friedman, M. 2014. Microwave heating inactivates Shiga Toxin (Stx2) in pH 7.4 buffer and in reconstituted fat-free Milk and adversely affects the nutritional value of cell culture medium. Journal of Agricultural and Food Chemistry. 62:3301-3305 DOI.org/10.1021/jf500278a.
Non-linear relationships between aflatoxin B1 levels and the biological response of monkey kidney vero cells - (Peer Reviewed Journal)
Rasooly, R., Hernlem, B.J., He, X. 2013. Non-linear relationships between aflatoxin B1 levels and the biological response of monkey kidney vero cells. Toxins. 5:1447-1461. doi:10.3390/toxins5081447.
Low levels of aflatoxin B1, ricin and milk enhance recombinant protein production in mammalian cells - (Peer Reviewed Journal)
Rasooly, R., Hernlem, B.J., Friedman, M. 2013. Low levels of aflatoxin B1, ricin and milk enhance recombinant protein production in mammalian cells. PLoS One. 8(8):e71682. doi:10.1371/journal.pone.0071682.
TNF as biomarker for rapid quantification of active Staphylococcus enterotoxin A in food - (Peer Reviewed Journal)
Rasooly, R., Hernlem, B.J. 2012. TNF as biomarker for rapid quantification of active Staphylococcus enterotoxin A in food. Sensors. 12:5978-5985. doi:10.3390/s120505978.
Liquid formulations sustaining the viability of a superior yeast strain Pichia anomala WRL-076 for mycotoxin control - (Abstract Only)
Hua, S.T., Hernlem, B.J., Yokoyama, W.H., Parfitt, D.E., Sarreal, S.L. 2012. Liquid formulations sustaining the viability of a superior yeast strain Pichia anomala WRL-076 for mycotoxin control. American Society for Microbiology General Meeting. [abstract].
Techniques for rapid detection and quantification of active foodborne Staphylococcus Enterotoxin(Abstract) - (Abstract Only)
Hernlem, B.J., Rasooly, R. 2012. Techniques for rapid detection and quantification of active foodborne Staphylococcus Enterotoxin(Abstract). Meeting Abstract. Poster no. B266.
Bacterial communities in urban aerosols collected with wetted-wall cyclonic samplers and seasonal fluctuations of live and culturable airborne bacteria - (Peer Reviewed Journal)
Ravva, S.V., Hernlem, B.J., Sarreal, C.Z., Mandrell, R.E. 2012. Bacterial communities in urban aerosols collected with wetted-wall cyclonic samplers and seasonal fluctuations of live and culturable airborne bacteria. Journal of Environmental Monitoring. 14(2):473-481. doi: 10.1039/clem10573d.
Cd154 As a Potential Early Molecular Biomarker for Rapid Quantification Analysis of Active Staphylococcus Enterotoxin A - (Peer Reviewed Journal)
Rasooly, R., Hernlem, B.J. 2011. CD154 as a potential early molecular biomarker for rapid quantification analysis of active Staphylococcus enterotoxin A. FEMS Immunology and Medical Microbiology. 64(2):169-174.
Auto-presentation of Staphylococcal enterotoxin A by mouse CD4+ T cells - (Peer Reviewed Journal)
Rasooly, R., Do, P.M., Hernlem, B.J. 2011. Auto-presentation of Staphylococcal enterotoxin A by mouse CD4+ T cells. Open Journal of Immunology. 1(1):8-14. doi:10.4236/oji.2011.11002.
Fluorescent viability stains to probe the metabolic status of aflatoxigenic fungus in dual culture of Aspergillus flavus and Pichia anomala - (Peer Reviewed Journal)
Hua, S.T., Brandl, M., Hernlem, B.J., Eng, J.G., Sarreal, S.L. 2010. Fluorescent viability stains to probe the metabolic status of aflatoxigenic fungus in dual culture of Aspergillus flavus and Pichia anomala. Mycopathologia. Available: http://www.springerlink.com/content/w0204147t65552h8/fulltext.pdf.
Vitality Stains and Real Time PCR Studies to Delineate the Interactions of Pichia anomala and Aspergillus flavus - (Abstract Only)
Hua, S.T., Brandl, M., Hernlem, B.J., Sarreal, S.L. 2010. Vitality Stains and Real Time PCR Studies to Delineate the Interactions of Pichia anomala and Aspergillus flavus. American Society for Microbiology General Meeting, San Diego, CA, May 23-27, 2010.
Dihaploid stocks for switchgrass isolated by a screening approach - (Peer Reviewed Journal)
Young, H.A., Hernlem, B.J., Anderton, A.L., Lanzatella-Craig, C., Tobias, C.M. 2010. Dihaploid stocks for switchgrass isolated by a screening approach. BioEnergy Research. 3:305-313.
Dual fluorochrome flow cytometric assessment of yeast viability - (Peer Reviewed Journal)
Hernlem, B.J., Hua, S.T. 2010. Dual fluorochrome flow cytometric assessment of yeast viability. Current Microbiology. 61:57-63.
Development of monoclonal antibodies specific for Ricinus agglutinins - (Peer Reviewed Journal)
Brandon, D.L., Hernlem, B.J. 2009. Development of monoclonal antibodies specific for Ricinus agglutinins. Food and Agricultural Immunology. 20(1):11-22
Application of flow cytometry and cell sorting to the bacterial analysis of environmental aerosol samples - (Peer Reviewed Journal)
Hernlem, B.J., Ravva, S.V. 2007. Application of flow cytometry and cell sorting to the bacterial analysis of environmental aerosol samples. Journal of Environmental Monitoring. 2007(9) 1317-1322.
Electrolytic Disinfection of Escherichia Coli and Coliform Bacteria in a Batch Cell with Dsa Electrodes - (Abstract Only)
Hernlem, B.J. 2006. Electrolytic disinfection of escherichia coli and coliform bacteria in a batch cell with dsa electrodes [Abstract]. ISAC XXIII International Congress. Abstract #229; Poster # P83.
Combination of Chlorine-Free Electrolytic and Photochemical Methods for Sterilization of Contaminated Waters - (Abstract Only)
Barashkov, N.N., El Fenni, M., Eisenberg, D., Eisenberg, S., Lam, L., Hernlem, B.J. Combination of chlorine-free electrolytic and photochemical methods for sterilization of contaminated waters [Abstract]. The 232nd ACS National Meeting. Paper ENVR 161.
Electrolytic Destruction of Urea in Dilute Chloride Solution Using Dsa Electrodes in a Recycled Batch Cell - (Peer Reviewed Journal)
Hernlem, B.J. 2005. "Electrolytic Destruction of Urea in Dilute Chloride Solution using DSA Electrodes in a Recycled Batch Cell." Water Research. 39(11):2245-2252
Electroflotation of Dairy Manure Lagoon Water: Chemical & Microbiological Effects - (Other)
Hernlem, B.J. 2004. Electroflotation of dairy manure lagoon water: chemical & microbiological effects. Meeting Abstract.
Disinfection and Solids Removal of Poultry Chiller Water by Electroflotation - (Peer Reviewed Journal)
Tsai, L.-S., Hernlem, B.J., Huxsoll, C.C. 2002. Disinfection and Solids Removal of Poultry Chiller Water by Electroflotation. Journal of Food Science. 67(6):2160-2164.
Chlorine Generation and Disinfection by Electroflotation - (Peer Reviewed Journal)
Hernlem, B.J., Tsai, L.-S. 2000. Chlorine Generation and Disinfection by Electroflotation. Journal of Food Science. 65(4):834-837.
Combined Electroflotation and Disinfection in Food Processing - (Abstract Only)
Hernlem, B.J., Tsai, L.-S., Huxsoll, C., Robertson, G. 2000. Combined Electroflotation and Disinfection in Food Processing. Paper Number 74-12, presented at the 2000 Institute of Food Technologists Annual Meeting, June 10-14, 2000, Dallas, TX. Available: http://ift.confex.com/ift/2000/techprogram/paper_4502.htm.
ARS News Articles
Last Modified: 11/28/2014