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United States Department of Agriculture

Agricultural Research Service

Key Publications
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High-Throughput Automated Integrated Molecular Biology Robotic Platforms

 

·         Hughes, S.R., Riedmuller, S.B., Mertens, J.A., Li, X-L., Bischoff,K.M., Cotta, M.A., and Farrelly, P.J.  Development of a liquid handler component for a plasmid-based functional proteomic robotic workcell. .J. Assoc. Lab. Automat. 10:287-300.  2005.

 

·         Hughes, S.R., Riedmuller, S.B., Mertens, J.A., Li, X-L, Bishoff, K.M., Qureshi, N., Cotta, M.A., and Farrelly, P.J.  High-throughput screening of cellulose F mutants from multiplexed plasmid sets using an automated plate assay on a functional proteomic robotic workcell.  Proteome Science. 4:10.  2006.

 

·         Hughes, S.R., Dowd, P.F., Hector, R.E., Riedmuller, S.B., Bartolett, S., Mertens, J.A., Qureshi, N., Liu, S., Bischoff, K.M., Li, X., Jackson Jr, J.S., Sterner, D., Panavas, T., Cotta, M.A., Farrelly, P.J., Butt, T.  High-throughput fully automated construction of a multiplex library of mutagenized open reading frames for an insecticidal peptide using a plasmid-based functional proteomic robotic workcell with improved vacuum system. Journal of Laboratory Automation. 12(4):202-212.  2007.

 

·         Hughes, S.R., Dowd, P.F., Hector, R.E., Panavas, T., Sterner, D.E., Qureshi, N., Bischoff, K.M., Bang, S.B., Mertens, J.A., Johnson, E.T., Li, X., Jackson Jr, J.S., Caughey, R.J., Riedmuller, S.B., Bartolett, S., Liu, S., Rich, J.O., Farrelly, P.J., Butt, T.R., Labaer, J., Cotta, M.A.  Lycotoxin-1 insecticidal peptide optimized by amino acid scanning mutagenesis and expressed as a co-product in an ethanologenic Saccharomyces cerevisiae strain. Journal of Peptide Science. 14(9):1039-1050.  2008.

 

·         Hughes, S.R., Sterner, D.E., Bischoff, K.M., Hector, R.E., Dowd, P.F., Qureshi, N., Bang, S.S., Grynavyski, N., Chakrabarty, T., Johnson, E.T., Dien, B.S., Mertens, J.A., Caughey, R.J., Liu, S., Butt, T.R., Labaer, J., Cotta, M.A., Rich, J.O.  Engineered Saccharomyces cerevisiae strain for improved xylose utilization with a three-plasmid SUMO yeast expression system. Plasmid. 61(1):22-38.  2009.

 

·         Hughes, S.R., Hector, R.E., Rich, J.O., Qureshi, N., Bischoff, K.M., Dien, B.S., Saha, B.C., Liu, S., Jackson Jr, J.S., Sterner, D.E., Butt, T.R., Labaer, J., Cotta, M.A. 2009. Automated yeast mating protocol using open reading frames from Saccharomyces cerevisiae genome to improve yeast strains for cellulosic ethanol production. Journal of the Association for Laboratory Automation. 8:190-199.  2009.

 

·         Hughes, S.R., Rich, J.O., Bischoff, K.M., Hector, R.E., Qureshi, N., Saha, B.C., Dien, B.S., Liu, S., Jackson Jr, J.S., Sterner, D.E., Butt, T.R., Labaer, J., Cotta, M.A.  Automated yeast transformation protocol to engineer S. cerevisiae strains for cellulosic ethanol production with open reading frames that express proteins binding to xylose isomerase identified using robotic two-hybrid screen. Journal of the Association for Laboratory Automation. 8:200-212.  2009.

 

Process for Rapid Automated Synthetic Optimized Open Reading Frame Assembly and Expression Using Amino Acid Scanning Mutagenesis

 

·         Hughes, S.R., Moser, B.R., Harmsen, A.J., Bischoff, K.M., Jones, M.A., Pinkelman, R., Bang, S.S., Tasaki, K., Doll, K.M., Qureshi, N., Liu, S., Saha, B.C., Jackson Jr, J.S., Cotta, M.A., Rich, J.O., Caimi, P.  Production of Candida antarctica lipase B gene open reading frame using automated PCR gene assembly protocol on robotic workcell and expression in ethanologenic yeast for use as resin-bound biocatalyst in biodiesel production. Journal of the Association for Laboratory Automation. 16(1):17-37. DOI: 10.1016/j.jala.2010.04.002.  2010.

 

·         Hughes, S.R., Butt, T.R., Bartolett, S., Riedmuller, S.B., Sterner, D., Farrelly, P.  Design and construction of a first-generation high-throughput integrated robotic molecular biology platform for bioenergy applications. Journal of the Association for Laboratory Automation. p. 292-307. DOI: 10.1016/j.jala.2011.04.004  2011.

 

·         Hughes, S.R., Moser, B.R., Robinson, S., Cox, E.J., Harmsen, A.J., Friesen, J.A., Bischoff, K.M., Jones, M.A., Pinkelman, R., Bang, S.S., Tasaki, K., Doll, K.M., Qureshi, N., Liu, S., Saha, B.C., Jackson, J.S., Cotta, M.A., Rich, J.O., Caimi, P.  Synthetic resin-bound truncated Candida antarctica lipase B for production of fatty acid alkyl esters by transesterification of corn and soybean oils with ethanol or butanol. Journal of Biotechnology. 159:69-77. DOI: 10.1016/j.jbiotec.2012.01.025.  2012.

  

Integreated Biorefinery Concepts

 

·         Gibbons, W.R., Hughes, S.R.  Integrated biorefineries with engineered microbes and high-value co-products for profitable biofuels production. In Vitro Cellular and Developmental Biology - Plants. 45:218-228.  2009.

 

·         Songstad, D.D., Lakshmanan, P., Chen, J., Gibbons, W., Hughes, S.R., Nelson, R. 2009. Historical perspective of biofuels: Learning from the past to rediscover the future. In Vitro Cellular and Developmental Biology - Plants. 45:189-192.

 

High Content UV-C Mutagenesis and High-throughput Screening of Improved Industrial Strains

 

·         Hughes, S.R., Gibbons, W.R., Bang, S.S., Pinkelman, R., Bischoff, K.M., Slininger, P.J., Qureshi, N., Kurtzman, C.P., Liu, S., Saha, B.C., Jackson, J.S., Cotta, M.A., Rich, J.O., Javers, J.E.  Random UV-C mutagenesis of Scheffersomyces (formerly Pichia) stipitis NRRL Y-7124 to improve anaerobic growth on lignocellulosic sugars. J Ind Microbiol Biotechnol. 39(1):163–173.  2012.


Last Modified: 2/27/2014
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