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United States Department of Agriculture

Agricultural Research Service

Methods & Techniques

Trangenic Murine Model- to evaluate the role of TLR4 in the development of insulin resistance. Various molecular biological and imaging techniques to study signaling pathways, gene expression and cellular responses: Cell culture, plasmid tranfection, confocal microscopy, lipid raft fractionation by sucrose gradient ultra-centrifugation, immunoprecipitation, western blot, ELISA, multiplex, and rtPCR techniques.



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Last Modified: 9/22/2011
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