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United States Department of Agriculture

Agricultural Research Service

Methods & Techniques

 

Methods in the Stephensen laboratory focus on evaluation of cellular immune responses in humans and in mice using a variety of methods.  Methods include the following:

·          Isolation of peripheral blood mononuclear cells or cell subsets (e.g., monocytes, total granulocytes, eosinophils, T-cell subsets) using density gradients, magnetic beads and FACS

·          Culturing primary cultures of lymphocytes, monocytes or granulocytes using Luminex or MSD array technology

·          Examination of cytokine production using cell culture supernatants or by intracellular cytokine staining and flow cytometric analysis

·          Evaluation of vitamin A status using HPLC methods

·          Western blot analysis of protein expression

·          RNA expression analysis using quantitative RT-PCR or microarray methods

 

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Last Modified: 9/23/2011
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