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United States Department of Agriculture

Agricultural Research Service

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Cold Storage of In Vitro Plants

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     Zee, Francis, R.T. Hamasaki, S.T. Nakamoto, L. Keith, K. Hummer, B. Reed  and Kawabata, A.  2010. Producing potted ornamental ohelo, Vaccinium.  UH-CTAHR OF-50 p. 1-8.

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Aynalem, H.A., T.L. Righetti, and B.M. Reed. 2006. Nondestructive evaluation of in vitro-stored plants:  A comparison of visual and image analysis. In Vitro Cellular and Developmental Biology-Plant. 42 562-567.

 

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Aynalem, H.A., T.L. Righetti, and B.M. Reed. 2006 Iron formulation affects in vitro storage of hops: an image analysis In Vitro Cellular and Developmental Biology - Plant 42: 405-410.

 

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Reed, B. 2002a. Photoperiod improves long-term survival of in vitro-stored strawberry plantlets HortScience. 37: 811-814.

 

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Reed, B.M. 1991a. Application of gas-permeable bags for in vitro cold storage of strawberry germplasm. Plant Cell Reports. 10: 431-434.

 

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Reed, B.M. 1992. Cold storage of strawberries in vitro: A comparison of three storage systems. Fruit Varieties Journal. 46: 98-102.

 

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Reed, B.M. 1993a. Improved survival of in vitro-stored Rubus germplasm. Journal of the American Society for Horticultural Science. 118: 890-895.

 

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Reed, B.M. 1999a. In vitro conservation of temperate tree fruit and nut crops, p. 139-153. In: E.E. Benson (ed.). Plant Conservation Biotechnology. Taylor and Francis, London.

 

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Reed, B.M. 1999c. In vitro storage conditions for mint germplasm. HortScience. 34: 350-352.

 

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Reed, B.M. 2005. In vitro storage of hops (Humulus L.) germplasm. Acta Horticulturae. 668: 250-256.

 

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Reed, B.M. and H.A. Aynalem. 2005. Iron formulation affects in vitro cold storage of hops. Acta Horticulturae. 668: 257-262.

 

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Reed, B.M. and Y. Chang. 1997. Medium- and long-term storage of in vitro cultures of temperate fruit and nut crops, p. 67-105. In: M.K. Razdan and E.C. Cocking (eds.). Conservation of Plant Genetic Resources In Vitro. Science Publishers, Inc., Enfield, NH, USA.

 

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Reed, B.M. and K. Hummer. 1995. Conservation of germplasm of strawberry (Fragaria species), p. 354-370. In: Y.P.S. Bajaj (ed.). Biotechnology in Agriculture and Forestry: Cryopreservation of Plant Germplasm I. Springer-Verlag, Berlin.

 

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Reed, B.M., J. DeNoma, J. Luo, Y. Chang, and L. Towill. 1998a. Cryopreservation and long-term storage of pear germplasm. In Vitro Cellular and Developmental Biology-Plant. 34: 256-260.

 

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Reed, B.M., N. Okut, J. D'Achino, L. Narver, and J. DeNoma. 2003. Cold storage and cryopreservation of hops (Humulus L.) shoot cultures through application of standard protocols. CryoLetters. 24: 389-396.

 

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Reed, B.M., C.L. Paynter, J. DeNoma, and Y. Chang. 1998b. Techniques for medium-and long-term storage of Pyrus L. genetic resources. Plant Genetic Resources Newsletter. 115: 1-4.

 

 

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Reed, B.M., Hummer, K., Gupta, S. and Change, Y.  2008.  Medium and Long-Term Storage of Rubus Germplasm.  Acta Horticulturae 777.  p.91-98. 


Contaminants of In Vitro Plants
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Buckley, P.M., T.N. DeWilde, and B.M. Reed. 1995. Characterization and identification of bacteria isolated from micropropagated mint plants. In Vitro Cellular and Developmental Biology. 31P: 58-64.

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Postman, J., J. DeNoma, and B.M. Reed. 2005. Detection and Elimination of Viruses in USDA Hop (Humulus lupulus) germplasm collection. Acta Horticulturae. 668: 143-148.

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Reed, B.M., P.M. Buckley, and T.N. DeWilde. 1995. Detection and eradication of endophytic bacteria from micropropagated mint plants. In Vitro Cellular and Developmental Biology. 31P: 53-57.

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Reed, B.M. and P. Tanprasert. 1995. Detection and control of bacterial contaminants of plant tissue cultures. A review of recent literature. Plant Tissue Culture and Biotechnology. 1: 137-142.

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Reed, B.M., Mentzer, J., Tanprasert, P. and X. Yu. 1998. Internal bacterial contamination of micropropagated hazelnut: identification and antibiotic treatment.  Plant Cell Tissue Organ Culture. 52: 67-70.

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Tanprasert, P. and B.M. Reed. 1997a. Detection and identification of bacterial contaminants from strawberry runner explants. In Vitro Cellular and Developmental Biology-Plant. 33: 221-226.

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Tanprasert, P. and B.M. Reed. 1997b. Determination of minimal bactericidal and effective antibiotic treatment concentrations for bacterial contaminants from micropropagated strawberries. In Vitro Cellular and Developmental Biology-Plant. 33: 227-230.

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Tanprasert, P. and B.M. Reed. 1998a. Detection and identification of bacterial contaminants from strawberry runner explants. Plant Cell Tissue Organ Culture. 52. 53-55.

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Wang, N. and B.M. Reed. 2003. Development, Detection, and Elimination of Verticillium dahliae in Mint Shoot Cultures   HortScience. 38: 67-70.

   
   
   

Cryopreservation

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 Benson, E.E., B.M. Reed, R.M. Brennan, K.A. Clacher, and D.A. Ross. 1996. Use of thermal analysis in the evaluation of cryopreservation protocols for Ribes nigrum L. germplasm. CryoLetters. 17: 347-362.

 PDF   Chang, Y., R.E. Barker, and B.M. Reed. 2000. Cold acclimation improves recovery of cryopreserved grass (Zoysia and Lolium sp.). CryoLetters. 21: 107-116.
 PDF  Chang, Y. and B.M. Reed. 1999. Extended cold acclimation and recovery medium alteration improve regrowth of Rubus shoot tips following cryopreservation. CryoLetters. 20: 371-376.
 PDF  Chang, Y. and B.M. Reed. 2000. Extended alternating-temperature cold acclimation and culture duration improve pear shoot cryopreservation. Cryobiology. 40: 311-322.
 PDF  Chang, Y. and B.M. Reed. 2001. Preculture conditions influence cold hardiness and regrowth of Pyrus cordata shoot tips after cryopreservation. HortScience. 36: 1329-1333.
 PDF  Dumet, D., W. Block, R. Worland, B. Reed, and E. Benson. 2000. Profiling cryopreservation protocols for Ribes ciliatum using differential scanning calorimetry. CryoLetters. 21: 367-378.
 PDF  Gupta, S. and B.M. Reed. 2006. Cryopreservation of shoot tips of blackberry and raspberry by encapsulation-dehydration and vitrification. CryoLetters. 27: 29-42.
 PDF  Luo, J. and B.M. Reed. 1997. Abscisic acid-responsive protein, bovine serum albumin, and proline pretreatments improve recovery of in vitro currant shoot-tip meristems and callus cryopreserved by vitrification. Cryobiology. 34: 240-250.
 PDF  Normah, M.N., B.M. Reed, and X. Yu. 1994. Seed storage and cryoexposure behavior in hazelnut (Corylus avellana L. cv. Barcelona). CryoLetters. 15: 315-322.
PDF  Reed, B.M. 1988. Cold acclimation as a method to improve survival of cryopreserved Rubus meristems. CryoLetters. 9: 166-171.
PDF  Reed, B.M. 1989. The effect of cold hardening and cooling rate on the survival of apical meristems of Vaccinium species frozen in liquid nitrogen. CryoLetters. 10: 315-322.
 PDF  Reed, B.M. 1990b. Survival of in vitro-grown apical meristems of Pyrus following cryopreservation. HortScience. 25: 111-113.
 PDF  Reed, B.M. 1993b. Responses to ABA and cold acclimation are genotype dependent for cryopreserved blackberry and raspberry meristems. Cryobiology. 30: 179-184.
 PDF  Reed, B.M. 1996. Pretreatment strategies for cryopreservation of plant tissues, p. 73-87. In: M.N. Normah, M.K. Narimah, and M.M. Clyde (eds.). In Vitro Conservation of Plant Genetic Resources. Universiti Kebangsaan Malaysia, Bangi, Selangor, Malaysia.
 PDF  Reed, B.M. 2001. Implementing cryogenic storage of clonally propagated plants. CryoLetters. 22: 97-104.
 PDF  Reed, B.M. 2002b. Implementing Cryopreservation for Long-Term Germplasm Preservation in Vegetatively Propagated Species, p. 22-36. In: L.E. Towill and Y.P.S. Bajaj (eds.). Biotechnology in Agriculture and Forestry: Cryopreservation of Plant Germplasm II. Springer-Verlag, Berlin.
 PDF  Reed, B.M. and Y. Chang. 1997. Medium- and long-term storage of in vitro cultures of temperate fruit and nut crops, p. 67-105. In: M.K. Razdan and E.C. Cocking (eds.). Conservation of Plant Genetic Resources In Vitro. Science Publishers, Inc., Enfield, NH, USA.
 PDF  Reed, B.M., J. DeNoma, J. Luo, Y. Chang, and L. Towill. 1998a. Cryopreservation and long-term storage of pear germplasm. In Vitro Cellular and Developmental Biology-Plant. 34: 256-260.
 PDF  Reed, B.M. 1999a. In vitro conservation of temperate tree fruit and nut crops, p. 139-153. In: E.E. Benson (ed.). Plant Conservation Biotechnology. Taylor and Francis, London.
 PDF  Reed, B.M. 2004.  Cryopreservation Manual.
 PDF  Reed, B.M., D. Dumet, J.S. DeNoma, and E.E. Benson. 2001a. Validation of cryopreservation protocols for plant germplasm conservation: a pilot study using Ribes L. Biodiversity and Conservation. 10: 939-949.
 PDF  Reed, B.M. and K. Hummer. 1995. Conservation of germplasm of strawberry (Fragaria species), p. 354-370. In: Y.P.S. Bajaj (ed.). Biotechnology in Agriculture and Forestry: Cryopreservation of Plant Germplasm I. Springer-Verlag, Berlin.
 PDF  Reed, B.M. and K.E. Hummer. 2001. Long-term storage of hazelnut embryonic axes in liquid nitrogen. Acta Horticulturae. 556: 177-179.
 PDF  Reed, B.M. and K.E. Hummer. 2002a. Cryopreservation of Ribes, p. 323-343. In: L.E. Towill and Y.P.S. Bajaj (eds.). Biotechnology in Agriculture and Forestry: Cryopreservation of Plant Germplasm II. Springer-Verlag, Berlin.
 PDF  Reed, B.M., I. Kovalchuk, S. Kushnarenko, A. Meier-Dinkel, K. Schoenweiss, S. Pluta, K. Straczynska, and E.E. Benson. 2004. Evaluation of critical points in technology transfer of cryopreservation protocols to international plant conservation laboratories.  CryoLetters. 25: 341-352.
 PDF  Reed, B.M. and H.B. Lagerstedt. 1987. Freeze preservation of apical meristems of Rubus in liquid nitrogen. HortScience. 22: 302-303.
 PDF  Reed, B.M., M.N. Normah, and X. Yu. 1994. Stratification is necessary for successful cryopreservation of axes from stored hazelnut seed. CryoLetters. 15: 377-384.
 PDF  Reed, B.M., N. Okut, J. D'Achino, L. Narver, and J. DeNoma. 2003. Cold storage and cryopreservation of hops (Humulus L.) shoot cultures through application of standard protocols. CryoLetters. 24: 389-396.
 PDF  Reed, B.M., C.L. Paynter, J. DeNoma, and Y. Chang. 1998b. Techniques for medium-and long-term storage of Pyrus L. genetic resources. Plant Genetic Resources Newsletter. 115: 1-4.
 PDF  Reed, B.M., L. Schumacher, D. Dumet, and E. Benson. 2005b. Evaluation of a modified encapsulation-dehydration procedure incorporating high sucrose pretreatments for the cryopreservation of Ribes germplasm In Vitro Cellular and Developmental Biology-Plant 41: 431-436.
 PDF  Reed, B.M., L. Schumacher, N. Wang, J. D'Achino, and R.E. Barker. 2006. Cryopreservation of bermudagrass germplasm by encapsulation dehydration. Crop Science. 46: 6-11.
 PDF  Reed, B.M., S. Schwanke, and R. Shala. 2001b. Pear seeds retain viability after liquid nitrogen immersion. HortScience. 36.
 PDF  Reed, B.M. and X. Yu. 1995. Cryopreservation of in vitro-grown gooseberry and currant meristems. CryoLetters. 16: 131-136.
 PDF  Uchendu, E. and Reed, B.  2008. A Comparative Study of Three Cryopreservation Protocols for Effective Storage of In Vitro-Grown Mint (Mentha SPP.). CryoLetters. 29(3):181-188.


In Vitro

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Bell, R.L. and Reed, B.M.  2002.  In vitro Tissue Culture of Pear: Advances in Techniques for Micropropagation and Germplasm Preservation.  Acta Horticulturae.  596:412-418.

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Kumar, M.B., Barker R.E., and Reed B.M. 1999. Morphological and Molecular Analysis of Genetic Stability in Micropropagated Fragaria x ananassa cv. Pocahontas. In Vitro Cellular and Developmental Biology-Plant. 35: 254-258.

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Hummer, K.E. and Reed, B.M.  1996.  Establishment and Operation of a Clonal Gene bank. In: Proceedings of a Consultation on Management of In Vitro and Field Germplasm Collections.  IPGRI, Rome

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Reed, B.M. 1990a. Multiplication of Rubus Germplasm In Vitro: A screen of 256 accessions. Fruit Varieties Journal. 44: 141-148.

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Reed, B.M. 1991. Micropropagation of Meadowfoam (Limnanthes spp.). Plant Cell Reports. 10: 94-96.

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Reed, B.M. 1995. Screening Pyrus Germplasm for In Vitro Rooting Response. HortScience. 30: 1292-1294.

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Reed, B.M. and Abdelnour-Esquivel A. 1991. The use of Zeatin to Initiate In Vitro Cultures of Vaccinium Species and Cultivars. HortScience. 26: 1320-1322.

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Reed, B.M., Engelmann F., Dulloo E., and Engels J. 2005. Technical Guidelines for the Management of Field and In Vitro Germplasm Collections, IPGRI/FAO/SGRP. , Rome, Italy, pp. 95.

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Reed, B.M. and Hummer K.E. 2002. Genetic Stability of Strawberries in Culture, p. 98-101. In: S.C. Hokanson and A.R. Jamieson (eds.). Strawberry Research to 2001. ASHS Press, Alexandria, VA.

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Reed, B.M. and Smith M.A.L. 1999. Designing a Micropropagation System: Workshop Presentations from the 1998 SIVB Congress on In Vitro Biology. In Vitro Cellular and Developmental Biology-Plant. 25: 275-284.

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Tsao, C. and. Reed, B.M. 2002. Gelling Agents, Silver Nitrate, and Sequestrene Iron Influence Adventitious Shoot and Callus Formation from Rubus Leaves. In Vitro Cellular and Developmental Biology-Plant. 38: 29-32.

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Tsao, C.W., Postman J.D., and Reed B.M. 2000. Virus Infections Reduce In Vitro Multiplication of 'Malling Landmark' Raspberry. In Vitro Cellular and Developmental Biology-Plant. 36: 65-68.

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Yeo, D.Y. and Reed B.M. 1995. Micropropagation of Three Pyrus Rootstocks. HortScience. 30: 620-623.

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Yu, X. and Reed B.M. 1993. Improved Shoot Multiplication of Mature Hazelnut (Corylus avellana L.) In Vitro Using Glucose as a Carbon Source. Plant Cell Reports. 12: 256-259.

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Yu, X. and Reed B.M. 1995. A Micropropagation System for Hazelnuts (Corylus species). HortScience. 30: 120-123.

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Seed
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Wada, S. and Reed, B.  2008.  Morphological Analysis of Rubus Seed.  Acta Horticulturae. 782 p. 67-74

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Reed, B.M., Schwanke, S., and Shala, R.  2001.  Pear Seeds Retain Viability after Liquid Nitrogen Immersion.  HortScience.  36(6):1121-1122.

   
   
   
   

Last Modified: 5/4/2011