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United States Department of Agriculture

Agricultural Research Service

Publications in PDF
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1 - Cold Storage of In Vitro Plants
2 - Contaminants of In Vitro Plants
3 - Cryopreservation
4 - In Vitro
5 - Seed


 Benson, E.E., B.M. Reed, R.M. Brennan, K.A. Clacher, and D.A. Ross. 1996. Use of thermal analysis in the evaluation of cryopreservation protocols for Ribes nigrum L. germplasm. CryoLetters. 17: 347-362.

 PDF   Chang, Y., R.E. Barker, and B.M. Reed. 2000. Cold acclimation improves recovery of cryopreserved grass (Zoysia and Lolium sp.). CryoLetters. 21: 107-116.
 PDF  Chang, Y. and B.M. Reed. 1999. Extended cold acclimation and recovery medium alteration improve regrowth of Rubus shoot tips following cryopreservation. CryoLetters. 20: 371-376.
 PDF  Chang, Y. and B.M. Reed. 2000. Extended alternating-temperature cold acclimation and culture duration improve pear shoot cryopreservation. Cryobiology. 40: 311-322.
 PDF  Chang, Y. and B.M. Reed. 2001. Preculture conditions influence cold hardiness and regrowth of Pyrus cordata shoot tips after cryopreservation. HortScience. 36: 1329-1333.
 PDF  Dumet, D., W. Block, R. Worland, B. Reed, and E. Benson. 2000. Profiling cryopreservation protocols for Ribes ciliatum using differential scanning calorimetry. CryoLetters. 21: 367-378.
 PDF  Gupta, S. and B.M. Reed. 2006. Cryopreservation of shoot tips of blackberry and raspberry by encapsulation-dehydration and vitrification. CryoLetters. 27: 29-42.
 PDF  Luo, J. and B.M. Reed. 1997. Abscisic acid-responsive protein, bovine serum albumin, and proline pretreatments improve recovery of in vitro currant shoot-tip meristems and callus cryopreserved by vitrification. Cryobiology. 34: 240-250.
 PDF  Normah, M.N., B.M. Reed, and X. Yu. 1994. Seed storage and cryoexposure behavior in hazelnut (Corylus avellana L. cv. Barcelona). CryoLetters. 15: 315-322.
PDF  Reed, B.M. 1988. Cold acclimation as a method to improve survival of cryopreserved Rubus meristems. CryoLetters. 9: 166-171.
PDF  Reed, B.M. 1989. The effect of cold hardening and cooling rate on the survival of apical meristems of Vaccinium species frozen in liquid nitrogen. CryoLetters. 10: 315-322.
 PDF  Reed, B.M. 1990b. Survival of in vitro-grown apical meristems of Pyrus following cryopreservation. HortScience. 25: 111-113.
 PDF  Reed, B.M. 1993b. Responses to ABA and cold acclimation are genotype dependent for cryopreserved blackberry and raspberry meristems. Cryobiology. 30: 179-184.
 PDF  Reed, B.M. 1996. Pretreatment strategies for cryopreservation of plant tissues, p. 73-87. In: M.N. Normah, M.K. Narimah, and M.M. Clyde (eds.). In Vitro Conservation of Plant Genetic Resources. Universiti Kebangsaan Malaysia, Bangi, Selangor, Malaysia.
 PDF  Reed, B.M. 2001. Implementing cryogenic storage of clonally propagated plants. CryoLetters. 22: 97-104.
 PDF  Reed, B.M. 2002b. Implementing Cryopreservation for Long-Term Germplasm Preservation in Vegetatively Propagated Species, p. 22-36. In: L.E. Towill and Y.P.S. Bajaj (eds.). Biotechnology in Agriculture and Forestry: Cryopreservation of Plant Germplasm II. Springer-Verlag, Berlin.
 PDF  Reed, B.M. and Y. Chang. 1997. Medium- and long-term storage of in vitro cultures of temperate fruit and nut crops, p. 67-105. In: M.K. Razdan and E.C. Cocking (eds.). Conservation of Plant Genetic Resources In Vitro. Science Publishers, Inc., Enfield, NH, USA.
 PDF  Reed, B.M., J. DeNoma, J. Luo, Y. Chang, and L. Towill. 1998a. Cryopreservation and long-term storage of pear germplasm. In Vitro Cellular and Developmental Biology-Plant. 34: 256-260.
 PDF  Reed, B.M. 1999a. In vitro conservation of temperate tree fruit and nut crops, p. 139-153. In: E.E. Benson (ed.). Plant Conservation Biotechnology. Taylor and Francis, London.
 PDF  Reed, B.M. 2004.  Cryopreservation Manual.
 PDF  Reed, B.M., D. Dumet, J.S. DeNoma, and E.E. Benson. 2001a. Validation of cryopreservation protocols for plant germplasm conservation: a pilot study using Ribes L. Biodiversity and Conservation. 10: 939-949.
 PDF  Reed, B.M. and K. Hummer. 1995. Conservation of germplasm of strawberry (Fragaria species), p. 354-370. In: Y.P.S. Bajaj (ed.). Biotechnology in Agriculture and Forestry: Cryopreservation of Plant Germplasm I. Springer-Verlag, Berlin.
 PDF  Reed, B.M. and K.E. Hummer. 2001. Long-term storage of hazelnut embryonic axes in liquid nitrogen. Acta Horticulturae. 556: 177-179.
 PDF  Reed, B.M. and K.E. Hummer. 2002a. Cryopreservation of Ribes, p. 323-343. In: L.E. Towill and Y.P.S. Bajaj (eds.). Biotechnology in Agriculture and Forestry: Cryopreservation of Plant Germplasm II. Springer-Verlag, Berlin.
 PDF  Reed, B.M., I. Kovalchuk, S. Kushnarenko, A. Meier-Dinkel, K. Schoenweiss, S. Pluta, K. Straczynska, and E.E. Benson. 2004. Evaluation of critical points in technology transfer of cryopreservation protocols to international plant conservation laboratories.  CryoLetters. 25: 341-352.
 PDF  Reed, B.M. and H.B. Lagerstedt. 1987. Freeze preservation of apical meristems of Rubus in liquid nitrogen. HortScience. 22: 302-303.
 PDF  Reed, B.M., M.N. Normah, and X. Yu. 1994. Stratification is necessary for successful cryopreservation of axes from stored hazelnut seed. CryoLetters. 15: 377-384.
 PDF  Reed, B.M., N. Okut, J. D'Achino, L. Narver, and J. DeNoma. 2003. Cold storage and cryopreservation of hops (Humulus L.) shoot cultures through application of standard protocols. CryoLetters. 24: 389-396.
 PDF  Reed, B.M., C.L. Paynter, J. DeNoma, and Y. Chang. 1998b. Techniques for medium-and long-term storage of Pyrus L. genetic resources. Plant Genetic Resources Newsletter. 115: 1-4.
 PDF  Reed, B.M., L. Schumacher, D. Dumet, and E. Benson. 2005b. Evaluation of a modified encapsulation-dehydration procedure incorporating high sucrose pretreatments for the cryopreservation of Ribes germplasm In Vitro Cellular and Developmental Biology-Plant 41: 431-436.
 PDF  Reed, B.M., L. Schumacher, N. Wang, J. D'Achino, and R.E. Barker. 2006. Cryopreservation of bermudagrass germplasm by encapsulation dehydration. Crop Science. 46: 6-11.
 PDF  Reed, B.M., S. Schwanke, and R. Shala. 2001b. Pear seeds retain viability after liquid nitrogen immersion. HortScience. 36.
 PDF  Reed, B.M. and X. Yu. 1995. Cryopreservation of in vitro-grown gooseberry and currant meristems. CryoLetters. 16: 131-136.
 PDF  Uchendu, E. and Reed, B.  2008. A Comparative Study of Three Cryopreservation Protocols for Effective Storage of In Vitro-Grown Mint (Mentha SPP.). CryoLetters. 29(3):181-188.

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Last Modified: 5/4/2011
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