Page Banner

United States Department of Agriculture

Agricultural Research Service

Natilla_research
headline bar

Dr. Angela Natilla (Postdoctoral Research Associate)
Molecular Plant Pathology
Research Molecular Biologist
angela.natilla@ars.usda.gov
Phone: (301) 504-5099- ext 268  Fax: (301) 504-5449

 Bldg 004,Room 017 BARC-WEST
10300 BALTIMORE AVENUE
BELTSVILLE, MD, 20705

 

Research Highlights:

Research focus:

Among our research interests is the use Virus-like-particles (VLPs) technology to design plant virus platforms for a new-generation of vaccines against prevalent and emergent animal diseases, production of biomaterials, chemical tools and diagnostics. Plant - VLPs are self-assembled structures derived from plant viral coat proteins that mimic the native architecture of the viruses but lack the viral genome. The VLPs are not able to reproduce as the viruses themselves, but can carry cellular RNAs. Plant viruses possess uniqueness to selfassemble in vitro and in vivo into VLPs with nanoscale dimensions and range of properties. These attributes include particulate shape and structure, specificity of symmetry, polyvalence as well as stability of properties under different ranges of temperature and pH.  

 

This versatility of the plant viruses allows their wide potential use as nanomaterials and, not surprisingly, the new viral nanotechnology field holds significant promise in agriculture and plant virology. 

 

In exploiting the spectrum of plant virus properties, we utilize a variety of protocols and strategies to modify a range of plant viruses to assume new functions. We also employ a range of techniques including genetic engineering and bio-conjugation reactions when incorporating foreign molecules on the surface of VLPs.

 

Diagram A                                                        Diagram B          

                                                                              

          

 

 

The genetic modification of the viral genome consists of insertion of the foreign sequence into the plant virus coat protein gene, and production of chimeric VLPs which carry the foreign peptide on the viral surface (A).  Bio-conjugation chemistries allow the conjugation of ligands (peptides) through cross-linking reactions to the reactive groups present on the exterior viral surface of VLPs (B).

 

 

 

 

 

 

 

 

 

 


Last Modified: 7/19/2013
Footer Content Back to Top of Page