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                                                             Intergenic Sequence Ribotype

Serotyping of Salmonella enterica species is the basis of national and international surveillance and communications.  It facilitates determining asscociations between the pathogen and sources and it gives some guidance in regards to preventing transmission.

The historic method used to serotype S. enterica is the antibody-based Kauffman-White (KW) scheme.  Postive results generate an antigenic formula based on structural details of the H-antigen of flagella and the O-antigen of lipopolysaccharide.

A major advantage of DNA analysis is that it is not impacted by variable expression of cell-surface antigens as are antibody-based agglutination assays like the KW scheme.  Major obstacles to genome typing of S. enterica becoming broadly available include expense, the need for highly specialized equipment, and, in some cases, sophisticated bioinformatics.  A discrete region within the S. enterica  has been shown to differentiate closely related serotypes.  The region of interest spans from the end of a ribosomal gene, across a 5S gene and includes the last base pair preceding a tRNA aspU ribosmal gene neighboring dkgB (previously yafB ).  This DNA-based method for assigning serotype to S. enterica at comparatively low cost and with readily accessible laboratory equipment commonly used for culturing and conducting the polymerase chain reaction (PCR) is described here.  Click on the link above to get more information.

                                                             Intergenic Sequence Ribotype

Serotyping of Salmonella enterica species is the basis of national and international surveillance and communications.  It facilitates determining asscociations between the pathogen and sources and it gives some guidance in regards to preventing transmission.

The historic method used to serotype S. enterica is the antibody-based Kauffman-White (KW) scheme.  Postive results generate an antigenic formula based on structural details of the H-antigen of flagella and the O-antigen of lipopolysaccharide.

A major advantage of DNA analysis is that it is not impacted by variable expression of cell-surface antigens as are antibody-based agglutination assays like the KW scheme.  Major obstacles to genome typing of S. enterica becoming broadly available include expense, the need for highly specialized equipment, and, in some cases, sophisticated bioinformatics.  A discrete region within the S. enterica  has been shown to differentiate closely related serotypes.  The region of interest spans from the end of a ribosomal gene, across a 5S gene and includes the last base pair preceding a tRNA aspU ribosmal gene neighboring dkgB (previously yafB ).  This DNA-based method for assigning serotype to S. enterica at comparatively low cost and with readily accessible laboratory equipment commonly used for culturing and conducting the polymerase chain reaction (PCR) is described here.  Click on the link above to get more information.

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