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Apple seeds from rare, wild trees in Central Asia offer a potential genetic bonanza for breeders looking for better flavor, disease resistance and other traits. ARS and university plant explorers, who collected more than 60,000 seeds last summer, are now analyzing traits of young plants grown from the seed. The trip was the third in a series to Central Asia, where the modern domestic apple is thought to have originated. The scientists collected seed and cuttings from the wild species Malus sieversii. It's a forerunner of the domestic apple, M. x domestica, which includes Red and Golden Delicious, McIntosh, Granny Smith and other popular varieties. One of the richest collecting sites was in the Tarbagatai mountain range, not previously visited by the American team. Tarbagatai, where winter temperatures dip to -40 degrees F, is the northernmost limit of M. sieversii. So, the new germplasm could someday extend apple growing farther north on this continent. The biggest apples the researchers collected--up to three inches in diameter--were found in the Tarbagatai area. Of the superior types collected, 84 percent had a pleasant, aromatic flavor and 70 percent were free of scab, a fungal disease.
Plant Genetic Resources, Geneva, NY
Philip L. Forsline, (315) 787-2390
A protein found in stored Golden Delicious apples can protect the fruit from storage rots. Also present in pears, tomatoes and raspberries, the protein stops the rampage of an enzyme produced by a pathogenic fungus, that attacks fruit after harvest. This is the first time that the protein, called polygalacturonase-inhibiting protein, has been purified from apples. Found in the apples by ARS scientists, the protein decreases as the fruit matures. The decrease occurs at a time when the fruit still needs protection from organisms that cause decay. Growers have long protected fruit from rots with fungicides, but many of the chemicals are no longer available because of health and environmental concerns. Scientists are cloning the gene responsible for producing the protein. They plan to manipulate the gene to produce large amounts of the protein as the fruit matures. It will be some time before they produce transgenic apple trees with the new gene, but once it has been inserted into tissue-cultured plants, they will be ready for field testing.
Horticultural Crops Quality Laboratory, Beltsville, MD
William S. Conway/Chenglin Yao, (301) 504-6128
A specific site on Chromosome 23 in Holstein dairy cows may hold a vital clue to whether a particular cow is more prone to mastitis infection. Mastitis is a bacterial infection of dairy cows that costs U.S. farmers more than $2 billion annually for treatment and lost milk production. Researchers studied the DNA of "grandsires" from seven different families of Holstein cattle, noting any genetic differences at three locations on each chromosome of the bulls. They discovered the lineup of genes at a specific site on Chromosome 23 differed between bulls whose daughters' milk contained high numbers of somatic cells--indicating a greater rate of mastitis--and bulls whose female offspring had lower somatic cell scores. Their conclusion: One particular variation in the DNA appears to be linked to lower somatic cell scores than in all other genetic lineups at that spot on Chromosome 23. Next, the researchers will check the DNA of Holsteins in a Maryland dairy herd, test those animals to see how well they resist mastitis infection, and decide whether the DNA predictors are accurate. If they can pinpoint which DNA lineups suggest reduced mastitis susceptibility, prospective breeding animals could be screened in advance for resistance to the disease.
Gene Evaluation and Mapping Laboratory, Beltsville, MD
Melissa S. Ashwell, (301) 504-8543
A map of the human genetic makeup is helping ARS scientists save time and money as they sort out and map chickens' genes. An initial genetic map for chickens has been developed. It will help breeders in the future produce birds known to carry the best genes for traits such as disease resistance and egg and meat production. To help complete the process of breeding a better chicken, scientists hope to find the exact genes by using information from the human genetic map. While the genetic makeup of chickens and humans isn't a particularly close match, researchers say there is enough similarity that information already available on the human genetic map can be used to search for specific genes in poultry. That's because during evolution, certain "chunks" of genes may have traveled together. So, a gene that's linked to arm length in humans might occupy approximately the same chromosomal location as genes related to wing growth in birds.
Avian Disease and Oncology Research, East Lansing, MI
Hans Cheng, (517) 337-6758
A variety of taro called Lila can triple the yield of this root crop on tropical farms. Taro produces underground stems, called corms, that are an excellent source of starch and minerals. The stems can be boiled, baked or roasted. When cooked, a corm tastes like a potato but has a softer texture. ARS scientists conducted field studies of Lila and found that, if properly fertilized and irrigated, it produces up to 17,770 pounds of commercial corms per acre--a 230 percent increase over the worldwide average for the crop. One reason for the yield increase: Lila was more efficient than other varieties in using soil nutrients. In general, taro is grown more widely in Pacific Island countries than in the Caribbean--partly because little attention has been paid to its potential compared to similar vegetables. In Puerto Rico, taro is grown on a small scale, though scientists say the findings could help boost taro production throughout the Caribbean and in other parts of the world.
Tropical Agriculture Research Station, Mayaguez, PR
Ricardo Goenaga, (787) 831-3435
Genes in a wild tobacco plant may give food and fiber crops a new defense against silverleaf whiteflies and other pests. Those genes could enable cotton, tomatoes and other crops to make the same natural chemicals the wild plant makes to kill the leaf-feeding insects. That's the objective of ARS scientists who have been analyzing the genetics of Nicotiana gossei--a wild Australian relative of commercial tobacco. N. gossei produces a number of insecticidal chemicals including sucrose esters. In lab and field tests, the esters have killed whiteflies, aphids, spider mites, pear psyllas and tobacco hornworms. Now, the scientists are pursuing genetic research, seeking to identify and isolate the sucrose-ester genes. Ultimately, scientists would insert the appropriate genes into crop species as a potential new form of nature-based pest control.
Soybean and Alfalfa Research Lab, Beltsville, MD
George Pittarelli, (301) 504-5723
Western Cotton Research Laboratory, Phoenix, AZ
David Akey, (602) 379-3524
Medical researchers may more quickly locate "jumping genes"--also known as transposons--that could have potential use in gene therapies. That's because ARS scientists and a software engineer at Silicon Graphics, Mountain View, CA, devised a transposon-finding mathematical formula. The team's intent is to design on-off switches for lab-built genes geared, for example, to protect potatoes from bacteria. But the team first needs to find more plant transposons. They then can devise new gene switches to mimic the transposons' genetic architecture. The ARS-designed formula, called an algorithm, instructs a supercomputer to search a gene database for patterns associated with transposons. The scientists recently tested the algorithm on the human gene data base. They knew this would be a tougher challenge than the much smaller database of plant genes. But the algorithm might also speed medical scientists' search for additional human transposons. New transposons might prove valuable for moving potentially beneficial genes into cells of patients suffering from AIDS, cancer or gene-linked diseases. The ARS team found two human transposon genes--one previously unknown, one only partially described before.
Crop Improvement and Utilization Research Unit, Albany, CA
William R. Belknap, (510)559-6072
For more information on Genetic Resources visit the National Germplasm Resources Laboratory) ARS/USDA.
Last updated: July 10, 1996
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Last Modified: 02/11/2002
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