Phenolic Compounds Studied
Citrus are a rich source of flavonoids (Robbins
1980). Determination of Citrus plant sources with high
concentrations of individual flavonoids is desirable in order
to study their biological properties. In this study, we determined
the percentages of flavonoids, coumarins, and psoralins in different
Citrus tissues. We have also quantified a number of specific
flavonoids in these tissues. The high-pressure liquid chromatography
patterns of phenolic compounds in members of the Citrus subtribe
were evaluated using sensitive HPLC technology.
This survey quantitatively evaluates the following flavonoids
- flavanone glycosides
- eriocitrin (eriodictyol–7–O–rutinoside)
- didymin (isosakuranetin–7–O–rutinoside)
- hesperidin (hesperetin–7–O–rutinoside)
- naringin (naringenin–7–O–neohesperidoside)
- naringin–6"–malonate
- naringin–4'–glucoside
- narirutin (naringenin–7–O–rutinoside)
- narirutin–4'–glucoside
- neoeriocitrin (eriodictyol–7–O–neohesperidioside)
- neohesperidin (hesperetin–7–O–neohesperidioside)
- poncirin (isosakuranetin–7–O–neohesperidioside)
- flavone glycosides
- diosmin (diosmetin–7–O–rutinoside)
- isorhoifolin (apiginin–7–O–rutinoside)
- rhoifolin (apiginin–7–O–neohesperidoside)
- flavonol glycoside
- rutin (quercetin–3–O–rutinoside)
The flavonoid chemical structures are depicted in figure
4.
In this report, we compare the concentrations and percentages
of these particular classes of phenolic compounds in various tissues
for several members of the Citrus subtribe. For each cultivar
studied, table 3 and table
4 provide estimates of the amounts of each phenolic compound
in leaf, flavedo, albedo, and juice vesicle tissues based on identification
by ultraviolet spectra. This information can be used to help differentiate
citrus species, and it demonstrates that the tissues have different
overall phenolic concentrations.
United States
Department of Agriculture
Agricultural
Research Service
The material on this page is in the public
domain.
Original posting: April 1, 1999.
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