...From the pages of Agricultural Research magazine
The Healing Touch of Cotton
Chemist Vince Edwards examines
a computer graphic image of an
enzyme model used in the design
of cotton-based chronic-wound
Cotton gauze has been used to dress wounds for hundreds of years because it is naturally soft, pliable, and absorbent. Now, a scientist from the Agricultural Research Service and his collaborators are using modern technology to further improve this cotton product and add to its list of beneficial characteristics. ARS has succeeded in patenting and licensing one type of improved gauze, and the researchers are working on creating another.
Chronic Wound Dressings
Vince Edwards, a chemist in the Cotton Textile Chemistry Research Unit at ARS' Southern Regional Research Center in New Orleans, Louisiana, has a background in medicinal chemistry. Although his research is focused on cotton chemistry, he keeps up to date on findings in the medical world as well. In 1997, he was reading the clinical journal Wound Repair and Regeneration when his interest was piqued by an article from scientists at the Medical College of Virginia (MCV) at Virginia Commonwealth University, in Richmond.
Technician Sarah Batiste
applies a finishing technique
to cotton gauze modified to
be a specialized dressing for
Researchers there, led by Dorne Yager, had identified a certain protease, or enzyme, present in elevated levels in bedsores, diabetic foot sores, and other wounds that won't heal. This particular protease, called elastase, breaks down connective tissue proteins that are essential to proper wound healing.
"In normal wounds, elastase and other proteases do a good job
of breaking down dying tissue and clearing debris from the site,"
says Kel Cohen, director of the Division of Plastic and Reconstructive
Surgery at the MCV Wound Healing Center. "But in chronic wounds,
these enzymes build up and overcompensate." In fact, in the chronic
skin ulcers common to long-term hospital patients, elastase can build
to more than 20 times normal levels.
Although occlusive dressingssynthetic bandages that limit water vapor loss and promote moist wound environmentsare being used more frequently on chronic wounds, cotton gauze remains a popular dressing material because of its low cost and high absorbency.
Chemist Phyllis Howley
examines the results of
an enzyme assay designed
to test how well modified
cotton wound dressings
sequester destructive proteases
found in chronic-wound fluid.
Edwards says, "After reading about the MCV research,
I thought we might be able to work together to make a new kind of cotton
dressing, since cotton fibers are an excellent carrier for wound-healing
He began collaborating with the Virginia medical group and in 1999 developed a way to chemically modify cotton gauze so that it selectively targets and soaks up elastase. "We bonded medicinal chemicals to cotton textiles to create a 'smart' wound dressing," he says.
The team has achieved promising results during in vitro
testing and expects to achieve similar results during in vivo testing.
Edwards explains their in vitro experiment:
"After receiving informed consent from a patient with a chronic
wound and the Virginia Commonwealth University Committee on the Conduct
of Human Research, we took fluid from the patient's pressure ulcer.
We clarified and buffered the wound fluid in a saline solution.
"Then we soaked both modified and unmodified gauze samples in
the wound fluid. The modified gauze, which specifically targeted elastase,
was able to sequester a lot more of it than the unmodified gauze,"
"We haven't yet tested these smart wound dressings on patients
with chronic wounds, but that's our next step," says Cohen.
Every year, about 1 million Americans develop chronic wounds, or pressure
ulcers, which result in patient-care costs approaching $750 million.
The modified gauze that Edwards and his collaborators developed is simple
and inexpensive to manufacture. It may ultimately be used in hospitals
and nursing homes.
Tissue Technologies, a company based in Richmond, Virginia, has licensed the technology. In 2002, it received a phase-one grant from the National Institutes of Health (NIH) through the Small Business Innovation Research program. In July 2002, the company applied for phase-two funding$1 millionwhich would be enough to pay for a clinical trial. Cohen, who is chief operating officer for Tissue Technologies, has served as a special research fellow at NIH and would oversee the clinical trial at a hospital in Richmond if they receive the funds.
Edwards and his collaborators have not limited themselves to improving
gauze for chronic wounds. They are also working on ways to improve cotton
burn dressings. Currently, they are developing a method to graft alginate,
which is produced by brown seaweed, to cotton fibers.
Cotton-based alginate dressings are absorbent and easily conform to
the shape and size of a wound. They are dry when applied but form a
soft gel when they interact with wound fluid and thus maintain a moist,
wound-healing environment. The wound dressing materials are designed
to accommodate movement around joints while providing water vapor transmissibility.
Edwards and his collaborators are achieving impressive results with
this research, although several more years of testing may be required
before it is introduced to the public. The Journal of Biomedical
Materials Research will soon publish an article about their work.By
Agricultural Research Service Information Staff.
This research is part of Quality and Utilization of Agricultural
Products, an ARS National Program (#306) described on the World Wide
Web at http://www.nps.ars.usda.gov.
J. Vincent Edwards is in the USDA-ARS Cotton Textile Chemistry Research Unit, Southern Regional Research Center, 1100 Robert E. Lee Blvd., New Orleans, LA 70179-0687; phone (504) 286-4360, fax (504) 286-4271.
"The Healing Touch of Cotton" was published in the January 2003 issue of Agricultural Research magazine.