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Contents
Fruit and Veggie Cut-Ups

Food technologist Alley Watada (left) and horticulturist Ling Qi, who is
visiting from China, prepare shredded carrots and other fresh-cut produce for
automated measurement of respiration rate and ethylene production.
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Fresh-cut produce, such as carrots, cauliflower, and broccoli, has become a
hot item with consumersnot just in the United States, but worldwide.
The International Fresh-cut Produce Association claims that
fresh cut produce is the fastest growing segment of the exploding market for
fresh fruits and vegetables. Sales of fresh-cuts in the United States are
projected to increase from $5.8 billion in 1994 to $19 billion by 1999.
"Fresh-cut products, which we used to call lightly or minimally
processed, have been around for many years," says Alley E. Watada, an ARS
food technologist. "But the types and quantity have expanded tremendously
over the past decade."
This is understandable when you consider that, in 1995, each of us ate close
to 28 pounds of fresh lettuce, 10 pounds of fresh carrots, and 28 pounds of
melons. John Love, who is with the USDA's Economic Research Service, estimates
that each American consumed about 300 pounds of fresh fruits and vegetables in
1996.
Watada believes that quality of fresh-cut produce has a lot to do with the
growth of the industry. "This industry is still relatively fragile in some
countries, and again, this has to do with product quality."
At the Horticultural Crops Quality Laboratory in Beltsville, Maryland,
Watada and his colleagues are developing methods to maintain and improve the
quality of fresh-cut fruits and vegetables.

Sanitation to control foodborne microorganisms is important to fresh-cut
produce quality. Food microbiologist Isabelle Babic (right) and technician
Laurie Gould will check honeydew melon samples applied to agar plates for
subsequent growth of disease-causing microbes.
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In the United States, most fresh-cut products are prepared by national or
regional processors located near the site where the crops are produced, which
means that the raw product is at peak quality. Maintaining that quality, Watada
says, depends on how processors control the temperature, relative humidity, and
sanitation of the product.
"When you cut produce, you leave a large surface area exposed to air
without any skin for protection against loss of water and attack by
microorganisms," he says. "The produce must be
shippedsome-times for several dayswhich makes shelf life vitally
important."
Since cut-up produce is much more perishable than the whole product, it must
be stored at a lower temperature.
Harvested fruits and vegetables are living tissue still undergoing
respiration and with a shelf life of about 7 to 14 days. The rate at which that
tissue deteriorates depends on the temperature to which it is subjected. So
Watada and colleagues just completed a study of respiration rates of more than
a dozen intact and fresh cut fruits and vegetables.
We found that to ensure quality, both fresh-cut and intact produce
should be handled and stored at or near 32°Fif the product is not
sensitive to chilling injury, Watada reports. And edible coatings
and proper packaging will help attain relative humidity close to 100 percent.
But we need more research on films or coatings that will adjust gas exchange,
or respiration, as the commoditys temperature changes.
Sanitation to control the growth of microorganisms is another very important
aspect of fresh-cut produce quality. Currently, processors rinse fresh-cuts in
a 50-to-200-parts-per-million chlorine solution. But chlorine doesn't always do
the trick, and it has come under scrutiny recently.
We found several types of bacteria and yeasts on fresh-cut spinach
that had been through a chlorine rinse, Watada says. The organisms
were inside broken cells or cells adjacent to broken tissue, where the chlorine
solution had not penetrated.
Joe Graziano agrees with Watada. He is president of Graziano Produce in
Portland, Oregon, and runs the largest fresh-cut produce company in the
northwestern United States.

To provide the proper combination of carbon dioxide and oxygen to fresh-cut
vegetable samples at various temperatures, technician Willard Douglas adjusts
the valves of a multigas mixture unit.
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"Although we use chlorine to sanitize fresh-cuts," says Graziano,
"we believe there could be something out there that might be more
effective. In the industry, we're always looking for something that will
improve the quality of our produce."
In a recent study with Hidemi Izumi of Kinki University in Wakayama, Japan,
Watada reduced growth of microorganisms on cut carrots by dipping them in a
calcium chloride solution.
"Calcium chloride has been beneficial in maintaining firmness of intact
fruit, so we tried it with carrot shreds, sticks, and slices and got excellent
results, he says.
In solutions of 0.5 percent and 1 percent calcium, cut carrots stored at
32° F, 41° F, and 50° F remained firm, with minimal increase in
growth of microorganisms, he reports. Although ideal storage temperature
is 32° F, we expanded the range because many lightly processed carrots are
held commercially at 41° F and sometimes 50° F.
The treatments increased the calcium content slightly in carrot sticks and
slices and substantially in the shreds, but the carrots still turned white in
storage. Watada and ARS colleagues have used sodium chloride solution on
shredded carrots to prevent such discoloration.
One of the problems with some fresh-cut produce is that the areas that have
been cut become white and translucent. Consumers associate this with aging and,
therefore, think the produce is not fresh. Carrots develop this white
appearance within 24 hours after being cut.
"Under a scanning electron microscope, we found that the abrasive
action of the knife blade shears, separates, and compresses the cells, causing
underlying damage to tissues," Watada explains. "Dehydration of a
large mass of cells and tissues causes the whitish color. We dipped shredded
carrots in different concentrations of sodium chloride solution, which
prevented discoloration and extended shelf life.
"We know that membrane degradation and repair processes are going on in
cut carrots, but we don't know anything about how these processes are
regulated," says Watada. "More research is needed, particularly at
the molecular level, to develop carrot breeding lines with desirable
characteristics for the fresh-cut industry."
The team of Watada and Izumi also tried the calcium solution on zucchini
squash, which is highly perishable and sensitive to chilling injury. At
32° F, chilling injury occurred, and at 50°F, natural deterioration
occurred; but at 41° F, both were minimal.
According to Watada, adding chlorine to the calcium helped bring about the
positive results. -- By Doris Stanley, ARS.
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