| Four Big Puzzles, Thousands
of Small Pieces
Kistler, along with molecular geneticists Todd J. Ward and Kerry O'Donnell
in the ARS Microbial Genomics and Bioprocessing Research Unit, Peoria,
Illinois, sequenced parts of several hundred Fusarium genes to
help map the genome. This work revealed that the fungus has only four
chromosomes. O'Donnell compares each chromosome to a huge jigsaw puzzle,
saying that the Broad Institute's task was to put the pieces together.
They succeeded, except for some odd pieces, which Kistler and Gale have
"We're 99.8 percent done. It's a remarkable achievement,"
says Kistler. "The Broad Institute used their computer to assemble
hundreds of thousands of little bits of DNA sequences into the complete
Fusarium genome. Other fungi have lots of simple DNA sequences
that are almost like spacers in the genome. These 'puzzle pieces' are
so simple that they could fit almost anywhere. So it can be nearly impossible
to find exactly where they fit," says Kistler. "Fortunately,
the Fusarium genome has very few of these ambiguous, generic
pieces. Almost all the pieces clearly fit in only one place."
Because of this, the sequencing is more complete than has been done
for any other fungus of this type. Kistler and his colleagues are encouraged
about finding a way to control scab disease using the genome information.
Two Maps Are Better Than One
"The Broad Institute's role was to take our raw genetic material
and do the actual sequencing needed to assemble the genome," says
Kistler. "ARS provided the genetic mapwhich locates the genes
in relation to each otherand the Broad Institute developed the
physical map, which shows the chemical makeup, or DNA sequences, that
make up the chromosomes. The two maps correspond well to each other,
validating the accuracy of the genome sequencing."
The genome map has been revised since its initial release. Both maps
have been combined and are now on the Broad Institute's web site at
"We're releasing information as fast as possible so it can reach
researchers anywhere in the world and immediately help them discover
genes they can use to genetically control scab," says Kistler.
Genome work always requires collaboration, Kistler says. "It's
too big a job for one person or lab to do. We enjoy this collaboration
because it's an integral part of what science is all about, and we all
profit from it."
At Michigan State University, associate professor Frances Trail focuses
on identifying genes that enable Fusarium to form the spores
that start the disease's spread each spring. At Purdue University, associate
professor Jin-Rong Xu specializes in finding genes that enable the fungus
to cause disease.
"Many Fusarium species can't cause disease," Kistler
notes, "so we want to find out which genes make it possible for
F. graminearum to cause scab. We are studying altered strains
of the fungus that don't form spores or don't cause disease. That will
help us find out which genes are needed to form spores and which genes
cause the disease.
"We know where only half the genes that produce the toxins are.
We are now using the genome sequence to find the other ones. Our overall
goal is to find out how this fungus causes scab, how it produces toxins,
and what environmental cues trigger scab. From there, we can devise
a strategy for preventing the disease. It's a case of getting to know
your enemy."By Don
Comis, Agricultural Research Service Information Staff.
This research is part of Plant, Microbial, and Insect Genetic Resources,
Genomics, and Genetic Improvement (#301), Crop Protection and Quarantine
(#304), and Food Safety (#108), three ARS National Programs described
on the World Wide Web at www.nps.ars.usda.gov.
H. Corby Kistler is with the
USDA-ARS Cereal Disease Laboratory,
1551 Lindig St., St. Paul, MN 55108; phone (612) 625-9774, fax (651)
and Todd J. Ward are
in the USDA-ARS Microbial
Genomics and Bioprocessing Research Unit, National Center for Agricultural
Utilization Research, 1815 North University St., Peoria, IL 61604-3999;
phone (309) 681-6383 [O'Donnell], (309) 681-6394 [Ward], fax (309) 681-6672.
"A First for Fusarium" was published in the
issue of Agricultural Research magazine.