Deadly Defenses Could Squelch Mosquitoes
Image from an electron
microscope of a nucleus in
Culex nigripalpus infected
with a baculovirus.
|You're strolling along, enjoying the
night air, when you're suddenly attacked! Whack! You get the assailantbut
other mosquitoes are on their way, seeking your blood as an evening snack.
ARS researchers are using new biological
controls to retaliate against the mosquito, an insect that can transmit
viruses, protozoan parasites, and filariae (small worms). Such defenses would
be useful here in the United States and vital in other countries where
mosquito-carried diseases such as malaria are a major health problem.
In the United States, other mosquito-transmitted viruses, such as West Nile
virus and St. Louis encephalitis, are making headlines.
West Nile virus, transmitted by the northern house mosquito, Culex
pipiens, first appeared in the United States in 1999. It was identified in
Uganda's West Nile District in 1937. The virus causes brain and spinal cord
inflammation in humans. West Nile virus infects many different bird species,
but it appears to be lethal to crows, jays, and hawks. Mosquitoes feed on birds
infected with the virus and then transmit it to humans and other animals.
St. Louis encephalitis is not new to the United States. It first appeared in
St. Louis, Missouri, in 1933, and is the most common mosquito-transmitted
disease in the United States. Mild infections cause fever or headaches.
Symptoms of severe infections include headaches, high fever, coma, tremors,
convulsions, paralysis, or even death.
Entomologists Jimmy Becnel
(foreground) and Don Barnard
examine ultrastructural features
of a mosquito baculovirus with
an electron microscope.
|A Baculovirus for a Blood
Entomologist James J. Becnel has found a new baculovirus (a virus specific to
arthropods) that kills mosquitoes carrying St. Louis encephalitis and West Nile
virus. Becnel, who is with the ARS Center for Medical, Agricultural, and
Veterinary Entomology's Mosquito and Fly Research Unit in Gainesville, Florida,
discovered the baculovirus in Florida in Culex nigripalpus and C.
fasciatus mosquitoes. The virus kills mosquitoes in the Culex genus,
which contains more than 500 species.
"No one has looked into using baculoviruses for mosquito control in 25
years. While baculoviruses have been used extensively for controlling
Lepidoptera (moth-type) species, baculoviruses from mosquitoes were
rare, and transmitting them was difficult," Becnel says. "We've been
able to get our new baculovirus to kill Culex nigripalpus mosquito
larvae in 48 to 72 hours. We can infect 60 to 70 percent of mosquitoes in
natural populations and 100 percent in the laboratory."
Culex nigripalpus larvae.
Magnesium is the key to Becnel's mosquito-killing tactics, because it activates
the baculovirus. Becnel developed a special cocktail that is added to water
where mosquitoes breed. Mosquitoes die when they drink the water containing the
virus. The higher the baculovirus concentration in the water, the longer
lasting the mosquito control.
"This baculovirus is very specificit only infects
mosquitoesand doesn't pose a threat to plants or wildlife," notes
"Another significant finding is that calcium prevents transmission of the
virus to mosquitoes. Understanding how to block as well as transmit the
baculovirus is crucial to developing successful control formulations for
various habitats. This knowledge may also be applied to other industries that
use baculoviruses," Becnel says.
Research associate Bettina
Moser examines a sample of
mosquito baculovirus purified
on a gradient.
|Becnel has filed for patents on his
discovery. He says this baculovirus could lead to a new, safe, cost-effective,
and specific biological control for mosquitoes that carry St. Louis
encephalitis and West Nile virus. Gainesville scientists are testing the
effectiveness of the baculovirus on Culex mosquitoes carrying West Nile
virus in the Northeast.
The Gainesville researchers are working with New York colleagues at ARS' Plum
Island Animal Disease Center to sequence the entire genome of the baculovirus
that infects Culex mosquitoes.
"We want to develop a germplasm repository that has genetic information on
a large number of mosquito viruses," says Donald R. Barnard, who heads the
mosquito unit. "Sequencing these viruses will help us determine which
genes are responsible for disease resistance or transmission in specific
mosquitoes. This will help us develop and test the baculovirus in other
Technician Heather Furlong
examines Culex nigripalpus
larvae for infection with
|Controlling an International
ARS researchers are also developing a biocontrol agent for yellowfever
mosquitoes, Aedes aegypti, which are pests of humans and livestock
worldwide. These mosquitoes transmit yellow fever virus and dengue virus, which
can cause dengue hemorrhagic fever. They develop in almost any container that
collects rainwater, such as barrels, tanks, old tires, cups, cans, and bottles.
The Gainesville experts are working with one of the first biological control
agents specific for A. aegypti mosquitoes. Edhazardia aedis is a
naturally occurring protozoan parasite that infects and kills these mosquitoes.
A scientist discovered the parasite in Puerto Rico in 1930. It was rediscovered
in Thailand in 1979. Becnel acquired regulatory permission to import the
parasite into the United States in 1993 for ARS researchers to study. When
adult female mosquitoes are infected, tiny spores pass the parasite to their
offspring. Most of the larvae die, although some survive to adulthood and
spread the infection to other mosquitoes.
|| In other mosquito species, however,
the parasite kills the larvae and is not spread to the next generation. But
A. aegypti adults will spread the pathogen as they lay eggs and move
from one water-filled container to another. Barnard says this is the first
pathogen-based biocontrol technology for container-bred mosquitoes.
Federal and state regulatory agencies have already granted ARS a permit to
release E. aedis in the United States, the first ever for an exotic
mosquito pathogen. "We completed field tests in Florida showing E.
aedis is highly effective against A. aegypti mosquitoes. It
completely eliminated test mosquito populations in 11 weeks," says Becnel.
Other Nations May Benefit
E. aedis could also help other countries with mosquito problems.
Brazilian researchers are especially interested in this biological control. ARS
and the Federative Republic of Brazil have established a formal agreement to
introduce E. aedis in Brazil to control A. aegypti mosquitoes.
"Dengue fever and malaria are major diseases transmitted by these
mosquitoes in Brazil," says Brazilian entomologist Paulo Vilarinhos,
coordinator of Brazil's National Biological Control Program. "Dengue is
present in all five Brazilian regions. And we expect the number of cases of
mosquito-related diseases to increase as a result of increased agricultural
production and more people moving near rivers and forests." These
conditions provide more breeding sites and hosts for mosquitoes.
A. aegypti infests 3,650 counties out of Brazil's total 5,500. Brazil
started a full-scale eradication program in 1997 using chemical controls.
Vilarinhos says, "Although we got more than a 50-percent reduction in
dengue cases from 1998, we'd like to reduce or replace chemical use with a
biological alternative. The mosquitoes are starting to develop resistance to
About 205,000 dengue cases are reported in Brazil each year. The last dengue
epidemic occurred in 1998 and resulted in 540,000 cases. Malaria is a major
problem in the country's northern region, with 600,000 cases reported annually.
Worldwide, dengue is one of the most important arthropod-transmitted human
viral diseases. About 50 million to 100 million cases of dengue hemorrhagic
fever are reported annually. A. aegypti mosquitoes are the major dengue
vector in urban tropical and subtropical regions of the world.
No vaccine is available for dengue. Barnard says the only effective prevention
method is to control the mosquitoes. He says this new biological control should
help do just that while reducing chemical use, environmental pollution, and
human and animal pesticide exposure.
ARS scientists are working with Vilarinhos to see whether E. aedis can
be established in natural mosquito populations in Brazil. "We're very
enthusiastic about its potential," says Vilarinhos.
The team will establish test sites in Brazil's five regions, each of which has
a different climate. The researchers want to know whether climate will alter
E. aedis' effectiveness. E. aedis will be deployed to the sites
in 2001. "We're hoping this biocontrol can collapse the Aedes
mosquito populations," says Vilarinhos.
"If our tests are successful in Brazil," says Barnard, "it could
have an impact on mosquitoes and mosquito-transmitted diseases throughout the
Weaver-Missick, Agricultural Research Service Information Staff.
This research is part of Arthropod Pests of Animals and Humans, an ARS
National Program (#104) described on the World Wide Web at
Donald R. Barnard and
James J. Becnel are with
the USDA-ARS Center for Medical,
Agricultural, and Veterinary Entomology, Mosquito and Fly Research Unit,
1700 SW 23rd Dr., Gainesville, FL 32604; phone (352) 374-5931, fax (352)
"Deadly Defenses Could Squelch Mosquitoes"
was published in the April 2001 issue
of Agricultural Research magazine.