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United States Department of Agriculture

Agricultural Research Service

Scientific Works of Art Reveal a Hidden World

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Scientific Works of Art Reveal a Hidden World


Scanning electron micrograph of a pair of red palm mites. Image was taken for the ARS Systematic Entomology Lab.
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It’s been said that a picture is worth a thousand words, and at the Agricultural Research Service’s Electron and Confocal Microscopy Unit (ECMU) in Beltsville, Maryland, this adage couldn’t be more true. Led by unit director Gary Bauchan, the ECMU is tasked with producing high-resolution images that provide a window to the extraordinary world of the unseen.

“We have observed viruses, bacteria, fungi, nematodes, insects, mites, and parasites that threaten global food security, and we’ve contributed to the discovery of how pathogens spread by helping elucidate their relationship to the environment, hosts, and vectors,” says Bauchan. “We’ve also described new biocontrol agents for the management of pathogens and characterized healthy and infected plant and animal tissues to discern the structural changes caused by pathogens.”


Scanning electron micrograph of a purple, red-eyed predatory mite, found on soil while hunting for small insects and mites. Image was taken for the ARS Systematic Entomology Lab.
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Other ECMU capabilities include tracking the development of genetically transformed plants using fluorescently tagged plant cells and tissues and contributing to improved food safety by determining the mechanisms by which bacteria, fungi, and parasites infect fresh produce.


Scientists conducting multifaceted studies at ARS’s Beltsville Agricultural Research Center (BARC) routinely call upon the microscopy experts in the unit to image all manner of specimens and samples. The researchers benefit not only in furthering their research objectives, but also by obtaining images to illustrate their journal articles, journal covers, grant proposals, web pages, and poster presentations.

 


A three-dimensional image, constructed from a series of images taken with a confocal fluorescence microscope, of a Cryptosporidium oocyst (bright green) inside a stoma (pore) of a spinach plant. The red spheres surrounding the oocyst are the plant’s chloroplasts.
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Needless to say, the microscopy unit’s services are in high demand and greatly appreciated. The latter was apparent from the turnout of BARC scientists and others who attended an October 11, 2012, ribbon-cutting ceremony presided over by Bauchan and Beltsville Area Director Joseph Spence. The event marked the completion of 2 years of renovations to the climate-controlled building that houses the ECMU and its prized collection of multi-million-dollar microscopes. Those include a low-temperature scanning electron microscope (LTSEM), a variable-pressure scanning electron microscope, two transmission electron microscopes, a confocal laser-scanning microscope (CLSM), and a Hirox digital video microscope. All are equipped with state-of-the-art digital cameras to speed the delivery of resulting images to researchers.

 

 


 


Scanning electron micrograph of a dust mite that feeds on dead human cells commonly found in homes. Image was taken for the ARS Systematic Entomology Lab.
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The ribbon-cutting ceremony also was in recognition of the expertise, creativity, and resourcefulness with which Bauchan, ECMU support scientists Charlie Murphy and Margaret Dienelt, and information technology specialist Christopher Pooley produce the images (at magnifications of up to 300,000 times) and ensure the integrity of the specimens for analysis by the researchers who submit them.

For example, when John Hammond, a plant pathologist at the U.S. National Arboretum’s Floral and Nursery Plants Research Unit, contacted the team for assistance, the result was a three-dimensional image of how viruses spread from leaf veins to adjacent leaf cells. The image, which was generated using fluorescently tagged viruses and a Zeiss 710 CLSM, accompanied an article in the August 2012 issue of the Journal of General Virology and illustrated the cover of the November 2012 issue.



In the ARS Electron and Confocal Microscopy Unit, in Beltsville, Maryland, (left to right) support scientist Charlie Murphy and entomologist Ron Ochoa observe a red velvet mite with a digital video camera microscope while lab director Gary Bauchan, plant pathologist John Hammond, and IT specialist Chris Pooley use a confocal laser scanning microscope to view tobacco leaves after gene insertion.
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Each of the unit’s microscopes offers unique capabilities and requires special handling to prepare specimens and samples prior to imaging. For systematic studies of mites, an LTSEM can be used. This necessitates flash-freezing a mite specimen (while still alive) at -321°F and coating it with platinum. In essence, the procedure freeze-frames the mite in time, allowing detailed examination of its features, physiology, behavior, and interaction with its immediate surroundings.

Imparting color to the high-magnification images helps further reveal critical, but often subtle, morphological differences between mite species, such as the size, shape, or number of setae (sensory organs) on their bodies. This capability has already proven invaluable to Beltsville scientists working with visiting scientist Jenny Beard, from the Queensland Museum in Australia, to differentiate species of mites in the genus Raoiella, members of which pose a significant threat to crops worldwide, including species of palm in the United States.

 


Scanning electron micrograph of a bean rust pustule ready to burst with infectious spores. Image was taken for the ARS Soybean Genomics and Improvement Lab.
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“At the Systematic Entomology Laboratory [SEL], our mite identifications support APHIS [USDA’s Animal and Plant Health Inspection Service] plant protection and quarantine efforts, state agricultural agents, extension agents, and other countries looking for information and help on mite pests that affect their crops—or crops they export to our country,” says mite expert Ron Ochoa, an ARS entomologist.

Images developed by SEL and ECMU have been used to develop an online identification key for flat mites of the world, tinyurl.com/flatmites. One year after the March 2012 launch of this website, there had been more than 86,392 visits to the web page, with inquiries from 196 countries. Ochoa notes that having access to the images is especially critical in making identifications that can become the impetus for regulatory decisions aimed at safeguarding U.S. agriculture, such as whether to treat, quarantine, or reject an agricultural import.


Scanning electron micrograph (SEM) of several Cryptosporidium oocysts (animal parasites, gold colored here) inoculated onto the surface of spinach to determine where they will settle.
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SEM of a Cryptosporidium oocyst in a spinach leaf stoma. Both images were taken for the ARS Environmental Microbiology and Food Safety Lab.
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Fluorescent confocal micrograph of the gut of a chicken with large cells containing intestinal parasites. Image was taken for the ARS Animal Parasitic Diseases Lab.
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Bauchan estimates that the ECMU collaborated on 40 different projects last year, and 2013 looks to be just as busy—not only in literally answering the call of science, but also capturing it visually, as the sampling of images illustrating this article can attest.—By Jan Suszkiw, Agricultural Research Service Information Staff.

This research is part of Plant Diseases, an ARS national program (#303) described at www.nps.ars.usda.gov.

 

 

 

 

 

 

 


Confocal micrograph of epidermal cells of a plant infected with a virus fluorescently labeled (yellow) to show virus movement from cell to cell within a leaf. Red spheres are chloroplasts from a second cell layer that’s not infected. Image was taken for the U.S. National Arboretum, ARS Floral and Nursery Plants Research Unit.
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Confocal micrograph of a soybean cell having two different genes inserted into it, each with a different color fluorescent tag. One attaches primarily to the nucleus (red) and the other to the cytoplasm (green). Image was taken for the Soybean Genomics and Improvement Lab.
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Gary Bauchan is in the USDA-ARS Electron and Confocal Microscopy Unit, Soybean Genomics and Improvement Laboratory, 10300 Baltimore Ave., Beltsville, MD 20705-2350; (301) 504-6649.

"Scientific Works of Art Reveal a Hidden World" was published in the August 2013 issue of Agricultural Research magazine.

Last Modified: 7/30/2013
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