|Ethidium Bromide SOP|
Ethidium Bromide (EtBr) wastes are commonly collected from ARS laboratories. Please dispose of this waste according to your state or local regulations. While most states treat this as non-hazardous waste, it is prudent that each laboratory evaluate all components of the EtBr waste stream and make a waste determination. In general the mutagenic properties of this waste stream may present a hazard if disposed down the drain or in regular trash. Please contact your ASHM, servicing CEPS, or LRPO for additional assistance. As always, your location is encouraged to edit this SOP to meet specific laboratory requirements.
Use a soap and water mixture, or detergent solution to clean or decontaminate laboratory work surfaces.
Use a UV light to survey work surfaces in the laboratory.
Solutions containing EtBr should be treated (many laboratories already do this) or it should be collected for disposal.
Test tubes, golves, paper trash:
If clearly contaminated with EtBr, this trash should be disposed of as hazardous waste. There are some locations that can dispose of this trash into their "burnable waste stream" because they specifically have access to a landlord incinerator that meets all requirements for incineration.
If the paper trash waste is not clearly contaminated with EtBr, such as some low concentration of gels, please confirm with your Location Radiation Protection Officer (LRPO) before trash disposal.
In all other cases:
Test tubes/glass/objects classified as RMW or sharps - always place in approved sharps container to prevent injury.
All Other Solids:
Label as "Ethidium Bromide Contaminated Debris"
Gels - Waste
Treatment of EtBr Waste
EtBr wastes can be treated in order to render them non-hazardous and suitable for disposal down the drain. We recommend the following procedure for treatment of aqueous wastes containing EtBr:
Supelco provides a product that will filter EtBr waste to remove it from solution. However, the resin used to filter the waste must be disposed as hazardous waste. For product information, call Supelco at 800-247-6628. Other companies specializing in separation technologies may also provide similar products.