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United States Department of Agriculture

Agricultural Research Service

Safety: Biohazardous Spill Protocol
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BIOHAZARDOUS SPILLS - OUTSIDE Containment (Biological Safety Cabinet)

  • Alert co-workers and leave lab area immediately.
  • Close door, post lab with "Do Not Enter" sign.
  • Remove and put contaminated garments into a container for autoclaving.
  • Wash hands/face with soap/antimicrobial agent.
  • Notify PI/supervisor.
  • Wait at least 30 minutes before re-entry (to allow aerosols to dissipate).
  • Upon re-entry, wear PPE (disposable gown, mask, gloves, etc.).
  • Carefully, pour an appropriate disinfectant solution (1:10 dilution of household bleach) around spill and cover spill with disinfectant soaked paper towels. Let stand for at least 20 minutes (taking care to not create aerosols while pouring).
  • Transfer all contaminated materials into an autoclave bag.
  • Wash and mop entire area with appropriate disinfectant.
  • Remove & discard PPE. Autoclave.
  • Shower or wash hands with soap/antimicrobial agent.
  • Report incident to the BSO (Biological Safety Officer), Occupational Health and the PI (if not already contacted).

BIOHAZARDOUS SPILLS - INSIDE Containment (Biological Safety Cabinet)

  • Immediately stop all work, but leave BSC blower fan on during clean-up.
  • Wear PPE (gloves, lab coat, eye protection).
  • Pour an appropriate disinfectant solution around spill and cover spill with disinfectant soaked paper towels.
  • Using paper towels and appropriate disinfectant detergent, wipe down walls, work surfaces and equipment.
  • Flood work surface & drain pan (type II, BSC) with disinfectant allow to stand at least 20 minutes.
  • Wipe up all excess disinfectant.
  • Autoclave all contaminated materials.
  • Report incident to the PI (if not already contacted).


Last Modified: 3/12/2010
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