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United States Department of Agriculture

Agricultural Research Service

Simon: Pubs: 99tag_0058
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Theor Appl Genet (1999) 99: 58-64

B. S. Vivek . P. W. Simon

Linkage relationships among molecular markers and storage root traits of carrot (Daucus carota L. ssp. sativus)

Received: 2 September 1998/Accepted: 28 November 1998

Abstract  A 109-point linkage map consisting of three phenotypic loci (P1, Y2, and Rs), six restriction fragment length polymorphisms (RFLPs), two random amplified polymorphic DNAs (RAPDs), 96 amplified fragment length polymorphisms (AFLPs), and two selective amplification of microsatellite polymorphic loci (SAMPL) was constructed for carrot (Daucus carota L. ssp. sativus; 2n=2x=18). The incidence of polymorphism was 36% for RFLP probes, 20% for RAPD primers, and 42% for AFLP primers. The overall incidence of disturbed segregation was 18%. Linkage relationships at a LOD score of 4.0 and theta=0.25 indicated 11 linkage groups. The total map length was 534.4 cM and the map was clearly unsaturated with markers spaced at 4.9 cM. AFLP P6B15 was 1.7 cM from P1, AFLP P1B34 was 2.2 cM from Y2, and AFLP P3B30XA was 8.1 cM from Rs.

Key words  Genetic map RFLP . AFLP . RAPD . SAMPL . Daucus carota L. ssp. sativus


Communicated by G. Wenzel

B. S. Vivek1 . P. W. Simon (envelope)
USDA-ARS, Vegetable Crops Research Unit,
Department of Horticulture, 1575 Linden Drive,
University of Wisconsin-Madison, Madison, WI 53705, USA
Fax: +1 608 262 4743
E-mail: psimon@facstaff.wisc.edu

Present address:
1CIMMYT, Lisboa 27, Apdo. Postal 6-641, Mexico D.F.,
06600, Mexico



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