|Simon: Pubs: 99euph0183|
Euphytica 105: 183-189, 1999.
Phylogeny and relationships in Daucus based on restriction fragment length polymorphisms (RFLPs) of the chloroplast and mitochondrial genomes
B.S.Vivek 1,2 & Philipp W. Simon 1,*
1USDA-ARS, Vegetable Crops Research Unit, Department of Horticulture, 1575 Linden Drive, University of Wisconsin-Madison, Madison, WI 53706, U.S.A.; 2Present address: CIMMYT, Lisboa 27, Apdo, Postal 6-641, Mexico D.F., 06600, Mexico; (*author for correspondence)
Received 9 April 1998; accepted 5 August 1998
Key words: carrot, cladistic, genetic diversity, phenetic
Chloroplast DNA (cpDNA) restriction site analysis of nine species of Daucus (covering four of five sections) and nine carrot accessions was done using 14 Petunia cpDNA clones as probes and 10 restriction enzymes. Cladistic analysis yielded a phylogeny generally concordant with a recent morphological classification. A cpDNA point mutation was found in the cultivated carrots (probe P10-Bgl II digest). Mitochondrial DNA (mtDNA) restriction site analysis of the same material using four maize mtDNA clones as probes and 10 enzymes exhibited extensive mitochondrial diversity in Daucus, both within and between sections.