We make these plasmid maps of the vectors and control elements used in our lab available for viewing and downloading. To view them, you must download and install Adobe Reader® from Adobe Systems, Inc. Please contact Dr. Bill Belknap for sequences and maps.

Control Elements

Granule Bound Starch Synthase (GBSS) promoter
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• HindII-EcoRI fragment in pCR vector
• High-level general tuber and root expression
• Ref: Van der Lief, et. al., Mol. Gen. Genet., 228:240-248, 1991.

Ubiquitin 3 Promoter
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• GUS expression cassette in pUC 19 vector
• Constitutive expression
• Ref: Garbarino et. al., Plant Physiology 109: 1371-1378, 1995.

Ubiquitin 3 poly ubiquitin fusion promoter
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• GUS expression cassette in pUC 19 vector
• High-level constitutive protein expression
• Ref: Garbarino et. al., Plant Physiology 109: 1371-1378, 1995.

Ubiquitin 3 Terminator
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• EcoRI insert in pCR vector
• All-purpose polyadenlyation signal

Patatin Class I Promoter in pUC 19 vector
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• Tuber specific expression
• Ref: Blundy, et. al., Plant Molecular Biology, 16:153-160, 1991.

Granule Bound Starch Synthase (GBSS) promoter + Ubiquitin3 terminator
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• HindIII-EcoRI expression cassette with BamHI cloning site for gene insertion
• Expression in tubers and roots

409s-MCS-409T Detail
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409s-MCS-409T in pBinplus/ARS
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Binary Vectors

pBINPLUS-ARS
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• Binary vector developed from pBINPLUS
• Complete sequence available in GenBank accession DQ320121

ubi3-GUS-ubi3T
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• in pBINPLUS/ARS