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United States Department of Agriculture

Agricultural Research Service

1 - Project Overview
2 - Vectors & Control Elements
3 - Laboratory Protocols
4 - Photo Gallery
Vectors & Control Elements

We make these plasmid maps of the vectors and control elements used in our lab available for viewing and downloading. To view them, you must download and install Adobe Reader® from Adobe Systems, Inc. Please contact Dr. Bill Belknap for sequences and maps.

Control Elements

Granule Bound Starch Synthase (GBSS) promoter
View Map(PDF)
  • HindII-EcoRI fragment in pCR vector
  • High-level general tuber and root expression
  • Ref: Van der Lief, et. al., Mol. Gen. Genet., 228:240-248, 1991.
Ubiquitin 3 Promoter
View Map(PDF)
  • GUS expression cassette in pUC 19 vector
  • Constitutive expression
  • Ref: Garbarino et. al., Plant Physiology 109: 1371-1378, 1995.
Ubiquitin 3 poly ubiquitin fusion promoter
View Map(PDF)
  • GUS expression cassette in pUC 19 vector
  • High-level constitutive protein expression
  • Ref: Garbarino et. al., Plant Physiology 109: 1371-1378, 1995.
Ubiquitin 3 Terminator
View Map(PDF)
  • EcoRI insert in pCR vector
  • All-purpose polyadenlyation signal
Patatin Class I Promoter in pUC 19 vector
View Map(PDF)
  • Tuber specific expression
  • Ref: Blundy, et. al., Plant Molecular Biology, 16:153-160, 1991.
Granule Bound Starch Synthase (GBSS) promoter + Ubiquitin3 terminator
View Map(PDF)
  • HindIII-EcoRI expression cassette with BamHI cloning site for gene insertion
  • Expression in tubers and roots
409s-MCS-409T Detail
View Map(PDF)

409s-MCS-409T in pBinplus/ARS
View Map(PDF)

Binary Vectors

pBINPLUS-ARS
View Map (PDF)
  • Binary vector developed from pBINPLUS
  • Complete sequence available in GenBank accession DQ320121
ubi3-GUS-ubi3T
View Map(PDF)
  • in pBINPLUS/ARS
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Last Modified: 5/10/2011
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