|Recent Research Accomplishments|
Created a B30 endolysin-lysostaphin fusion protein that is effective at killing both Streptococcal and Staphylococcal mastitis causing pathogens. Patent submitted.
Modified SAGE techniques for small and limited sample sizes.
Confirmed that primary NT bovine embryo-derived trophectoderm cell cultures produce less interferon-tau, the 'recognition of pregnancy' signal protein of the cow, compared to IVP embryo-derived cultures.
Created many new bovine trophectoderm cell lines from NT, IVP, parthenogenetic, and in vivo embryos.
Demonstrated that genetic engineering can be used to protect an important livestock species against an economically significant disease.
Established 2-D electrophoresis and mass spectrometry technologies in the laboratory.
Identified more than 800 different differentially expressed genes in hydrops placentl tissues.
Created model to explain the pathogenesis of the disease.
Generated bovine embryonic stem cell lines from NT and IVF embryos.
Improved oocyte activation protocols.
Developed a quantitative method for DNA methylation and histone acetylation in donor cells.
Produced multiple litters of normal piglets, esablishing greater than 60% pregnancy rate, with litter sized of up to 10 pigs per litter.
Improved surgical embryo transfer technique by introducing trans-oviductal uterine catheterization.
Determined that the dispersed testicular cells can be cultured for up to 2 wk.
Developed a novel procedure for removing the cryoprotectant glycerol from frozen-thawed chicken semen to enable the immediate banking of semen from genetically-valuable and/or unique research chicken lines.
Discovered the presence of a class of molecules, aquaporins, which regulate water transport in poultry male reproductive tract.