NCGRP Plant Research Unit Laboratories

Cryobiology of Vegetative Tissues | Cryobiology of Cells
Seed Quality and Biophysics | Population Genetics

Cellular and Molecular Aspects of Plant Cryopreservation

Lab Mission

Promote preservation technology of plant tissues through an understanding of cellular and molecular contributions to stress tolerance and genetic diversity.

Staff

Gayle Volk  Adam Henk   Jackie Harris Ann Caspersen

Principal Investigator Molecular phylogenetics, high-throughput genotyping, gene expression   Cryopreservation, plant tissue culture Microscopy, protoplast culture

Selected Research Projects

• Garlic genetic diversity
• Preservation of garlic under short-term condition
• Cryopreservation of vegetatively propagated crops
• Genetic diversity and erosion in wild relatives of fruit
• Damage and protection from exogenous cryoprotective solutions
• Gene expression patterns and cellular changes during acquisition of tolerance to cryoexposure

GAS c DNA alignments.txt

Non-truncated GAS alignments.txt

Publications Highlights

Volk, G.M., A. Henk, and C.M. Richards. 2004. Genetic diversity among US garlic clones as detected using AFLP methods. Journal of the American Society for Horticultural Science 129:559-569.

Volk, G.M., N. Maness, and K. Rotindo. 2004. Crypreservation of garlic (Allium sativum L.) using plant vitrification solution 2. CryoLetters 25:219-226.

Volk, G.M., C.M. Richards, A.A. Reilley, A.D. Henk, P.L. Forsline, and H.S. Aldwinckle. 2005. Ex situ conservation of vegetatively-propagated species: Development of a seed-based core collection for Malus sieversii. Journal of the American Society for Horticultural Science 130: (in press).