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United States Department of Agriculture

Agricultural Research Service

Germination Test Methods
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1 - Main area
2 - Amaranth: D. Brenner, Curator
3 - Maize: M. Millard, Curator
4 - Oilseeds: L. Marek, Curator
5 - Ornamentals: M. Widrlechner, Curator
6 - Vegetables: K. Reitsma, Curator
7 - Seed Testing Papers
8 - General & Historic
Vegetables: K. Reitsma, Curator


Vegetables Curator Group logo

 Click here for information on the seed testing papers we use.
 Contact Curator Kathy Reitsma.


Chicorium

491000

Cucurbita 490384
Cucumis 490382
Daucus 493996
Ocimum 490973
Pastinaca 490972


ENO: 491000  Chicorium

Duration

Temperature

Fluorescent Lights

12 hours

30 ºC

ON

12 hours

20 ºC

OFF

Seeds are placed on top of two blue blotter papers moistened with 0.1% KNO3 in plastic boxes (5" x 5-1/4"). Let seeds imbibe overnight at room temperature. Transfer to germinator with temperatures alternating alternating between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 5 and 14 days after start of test.

 

 TOP

ENO: 490384   Cucurbita

Duration

Temperature

Fluorescent Lights

12 hours

30 ºC

OFF

12 hours

20 ºC

OFF

Seeds are placed in folded paper towels moistened with tap water (substrate on dry side) and placed in plastic tubs. These are enclosed by a black plastic bag to exclude light. Transfer to germinator with temperatures alternating between 30 ºC for 12 hours and 20 ºC for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Counts are done 4 and 8 days after start of test.

 

 TOP

ENO: 490382   Cucumis

Duration

Temperature

Fluorescent Lights

12 hours

30 ºC

OFF

12 hours

20 ºC

OFF

Seeds are placed in folded paper towels moistened with tap water (substrate on dry side) and placed in plastic tubs. These are enclosed by a black plastic bag to exclude light. Transfer to germinator with temperatures alternating between 30 ºC for 12 hours and 20 ºC for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Counts are done 4 and 8 days after start of test.

 

 TOP

ENO: 493996   Daucus

Duration

Temperature

Fluorescent Lights

12 hours

25 ºC

ON

12 hours

15 ºC

OFF

Seeds are placed on top of 2 blue blotter papers moistened with tap water in plastic boxes (5" x 5-1/4"). Let sit overnight at room temperature. Transfer to germinator with temperatures alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Counts are done 7, 14, 21, and (if needed) 28 days after start of test.

 

 TOP

ENO: 490973  Ocimum

Duration

Temperature

Fluorescent Lights

12 hours

30 ºC

ON

12 hours

20 ºC

OFF

Seeds are placed on top of 2 blue blotter papers moistened with 0.1% KNO3 in plastic boxes (5" x 5-1/4"). Let sit overnight at room temperature. Transfer to germinator with temperatures alternating alternating between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7 and 14 after start of test.

 

 TOP

ENO: 490972   Pastinaca

Duration

Temperature

Fluorescent Lights

12 hours

25 ºC

ON

12 hours

15 ºC

OFF

Seeds are placed in folded paper towels moistened with tap water and placed in plastic tubs. Let sit overnight at room temperature. Transfer to germinator with temperatures alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Counts are done at 7, 14, and 21 days after start of test.

 

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Last Modified: 3/8/2012
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