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Chicorium	491000
Cucurbita	490384
Cucumis	490382
Daucus	493996
Ocimum	490973
Pastinaca	490972

ENO: 491000  Chicorium
Duration	Temperature	Fluorescent Lights
12 hours	30 ºC	ON
12 hours	20 ºC	OFF
Seeds are placed on top of two blue blotter papers moistened with 0.1% KNO3 in plastic boxes (5" x 5-1/4"). Let seeds imbibe overnight at room temperature. Transfer to germinator with temperatures alternating alternating between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 5 and 14 days after start of test.

 

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ENO: 490384   Cucurbita
Duration		Temperature	Fluorescent Lights
12 hours		30 ºC	OFF
12 hours		20 ºC	OFF
Seeds are placed in folded paper towels moistened with tap water (substrate on dry side) and placed in plastic tubs. These are enclosed by a black plastic bag to exclude light. Transfer to germinator with temperatures alternating between 30 ºC for 12 hours and 20 ºC for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Counts are done 4 and 8 days after start of test.

 

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ENO: 490382   Cucumis
Duration		Temperature	Fluorescent Lights
12 hours		30 ºC	OFF
12 hours		20 ºC	OFF
Seeds are placed in folded paper towels moistened with tap water (substrate on dry side) and placed in plastic tubs. These are enclosed by a black plastic bag to exclude light. Transfer to germinator with temperatures alternating between 30 ºC for 12 hours and 20 ºC for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Counts are done 4 and 8 days after start of test.

 

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ENO: 493996   Daucus
Duration		Temperature	Fluorescent Lights
12 hours		25 ºC	ON
12 hours		15 ºC	OFF
Seeds are placed on top of 2 blue blotter papers moistened with tap water in plastic boxes (5" x 5-1/4"). Let sit overnight at room temperature. Transfer to germinator with temperatures alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Counts are done 7, 14, 21, and (if needed) 28 days after start of test.

 

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ENO: 490973  Ocimum
Duration	Temperature	Fluorescent Lights
12 hours	30 ºC	ON
12 hours	20 ºC	OFF
Seeds are placed on top of 2 blue blotter papers moistened with 0.1% KNO3 in plastic boxes (5" x 5-1/4"). Let sit overnight at room temperature. Transfer to germinator with temperatures alternating alternating between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7 and 14 after start of test.

 

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ENO: 490972   Pastinaca
Duration		Temperature	Fluorescent Lights
12 hours		25 ºC	ON
12 hours		15 ºC	OFF
Seeds are placed in folded paper towels moistened with tap water and placed in plastic tubs. Let sit overnight at room temperature. Transfer to germinator with temperatures alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Counts are done at 7, 14, and 21 days after start of test.

 

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