Click here for information on the seed testing papers we use. Contact Curator Laura Marek.
ENO: 493335 Alliaria, Erysimum, Hesperis, Sinapis 
Duration 
Temperature 
Fluorescent Lights 
7 days 
4 ºC 
 
7 days 
12 hours 
30 ºC 
ON 
12 hours 
20 ºC 
OFF 
14 days + 
4 ºC 
 
7 days 
12 hours 
30 ºC 
ON 
12 hours 
20 ºC 
OFF 
Seeds are placed on top of 2 blue blotter papers moistened with tap water in plastic boxes (5" x 51/4"); let sit overnight at room temperature. Prechill at 4 ºC for 7 days. Transfer to germinator with temperatures alternating between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Leave at this regimen for 7 days. Place germination boxes back in cold room for at least 14 days  leave until seeds begin to germinate in cold room. Transfer back to 20/30 ºC, as before. Score immediately and at 7, 14, and 21 days. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. 
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ENO: 493339 Alyssum, Berteroa, Crambe, Eruca, Erucastrum, Isatis

Duration 
Temperature 
Fluorescent Lights 
7 days 
4 ºC 
 
12 hours 
20 ºC 
ON 
12 hours 
20 ºC 
OFF 
Seeds are placed between 2 blue blotter papers moistened with 0.1% KNO_{3} solution in plastic boxes (5" x 51/4") and kept at room temperature overnight. Prechill at 4 ºC for 7 days. Transfer to germinator set at constant 20 ºC with light for 12 hours and dark for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur weekly after start of test. 
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ENO: 491490 Asters 
Duration 
Temperature 
Fluorescent Lights 
14 days 
4 ºC 
 
16 hours 
25 ºC 
ON 
8 hours 
15 ºC 
OFF 
Seeds are placed on top of 2 blue blotter papers moistened with 1 mM GA_{3} in plastic boxes (5" x 51/4"); let sit overnight at room temperature. Prechill at 4 ºC for 14 days. Transfer to germinator with temperatures alternating alternating between 25 ºC with light for 16 hours and 15 ºC in darkness for 8 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of the germination test.
Additional comments: Madia prefers 15 ºC constant and dark environment. 
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ENO: 490360 Biscutella, Linum flavum 
Duration 
Temperature 
Fluorescent Lights 
12 hours 
30 ºC 
ON 
12 hours 
20 ºC 
OFF 
Seeds are placed on top of 3 blotter papers (1 blue, 2 white – in contact with seeds) moistened with 0.1% KNO_{3} in plastic boxes (5" x 51/4"); let sit overnight at room temperature. Transfer to germinator with temperatures alternating alternating between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of test. 
TOP
ENO: 178 Brassica (carinata, napus, rapa) 
Duration 
Temperature 
Fluorescent Lights 
12 hours 
25 ºC 
ON 
12 hours 
15 ºC 
OFF 
Seeds are placed between blotter papers moistened with 0.1% KNO_{3} in plastic boxes (5" x 51/4") with the following arrangement:
(top) 1 blue paper seeds 1 white paper (bottom) 1 blue paper
Let seeds imbibe overnight at room temperature; prechill at 4 ºC for 14 days. Transfer to germinator with temperatures alternating alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. (Alternatively, 2 reps of 100 seeds.) The first germination count occurs between 3 and 7 days and the last count is on the 14^{th} day after start of test. 
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ENO: 181 Brassica (juncea, tournefortii)

Duration 
Temperature 
Fluorescent Lights 
7 days 
10 ºC 
 
12 hours 
25 ºC 
ON 
12 hours 
15 ºC 
OFF 
Seeds are placed on top of 2 blue blotter papers moistened with 0.1% KNO_{3} solution in plastic boxes (5" x 51/4") and kept at room temperature overnight. Prechill at 10 ºC for 7 days. Transfer to germinator with temperatures alternating alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. The first germination count occurs between 3 and 7 days and the last count is on the 14^{th} day after start of the germination test. 
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ENO: 181 Brassica nigra

Duration 
Temperature 
Fluorescent Lights 
3 days 
10 ºC 
 
12 hours 
25 ºC 
ON 
12 hours 
15 ºC 
OFF 
Seeds are placed on top of 2 blue blotter papers moistened with 0.1% KNO_{3} solution in plastic boxes (5" x 51/4") and kept at room temperature overnight. Prechill at 10 ºC for 3 days. Transfer to germinator with temperatures alternating alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. The first germination count occurs between 3 and 7 days and the last count is on the 14^{th} day after start of the germination test. 
TOP
ENO: 493043 Brassica repanda 
Duration 
Temperature 
Fluorescent Lights 
12 hours 
30 ºC 
ON 
12 hours 
20 ºC 
OFF 
Seeds are placed on top of 3 blotter papers (2 blue, 1 white – in contact with seeds) moistened with 0.1% KNO_{3} in plastic boxes (5" x 51/4") and kept at room temperature overnight. Transfer to germinator with temperatures alternating alternating between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of test. 
TOP
ENO: 493220 Camelina, Goldbachia 
Duration 
Temperature 
Fluorescent Lights 
14 days 
4 ºC 
 
12 hours 
25 ºC 
ON 
12 hours 
15 ºC 
OFF 
Seeds are placed between blotter papers moistened with 0.1% KNO_{3} in plastic boxes (5" x 51/4") with the following arrangement:
(top) 1 blue paper seeds 1 white paper (bottom) 1 blue paper
Let seeds imbibe overnight at room temperature; prechill at 4 ºC for 14 days. Transfer to germinator with temperatures alternating alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. One germination count occurs 14 days after start of test; no other counts required. 
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ENO: 493034 Cuphea 
Duration 
Temperature 
Fluorescent Lights 
12 hours 
25 ºC 
ON 
12 hours 
15 ºC 
OFF 
Seeds are placed on top of 2 blue blotter papers moistened with tap water in plastic boxes (5" x 51/4"); let sit for 48 hours at room temperature. Transfer to germinator with temperatures alternating alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle.
Rep and sample size depends on quantity of seed available: If > 2500 sds, then 4 reps of 50 sd for total of 200 seeds. If > 1500 & < 2500 , then 4 reps of 25 sd for total of 100. If < 1500, then do not run germination test.
Germination counts occur at 7, 14, and 21 days after start of test. 
TOP
ENO: 492112 Euphorbia 
Duration 
Temperature 
Fluorescent Lights 
14 days 
4 ºC 
 
12 hours 
30 ºC 
ON 
12 hours 
20 ºC 
OFF 
Seeds are placed on top of 2 blue blotter papers moistened with tap water in plastic boxes (5" x 51/4"); let sit overnight hours at room temperature. Prechill at 4 ºC for 14 days. Transfer to germinator with temperatures alternating alternating between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 6 and 16 days after start of test. 
TOP
ENO: 105 Helianthus (cultivated) 
Duration 
Temperature 
Fluorescent Lights 
12 hours 
30 ºC 
ON 
12 hours 
20 ºC 
OFF 
Seeds are placed in folded paper towels^{1} moistened with tap water and placed in plastic tubs. Transfer to germinator with temperatures alternating between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle.
Rep and sample size depends on quantity of seed available: If > 1500 sds, then 4 reps of 50 sd for total of 200 seeds. If > 1200 & < 1500 , then 4 reps of 25 sd for total of 100. If < 1200, then do not run germination test.
Germination counts occur at 5 and 14 days after start of test.
^{1}Seeds are wrapped in 2 sheets of germination toweling and replicated four times. The four replications are rubber banded together and eight accessions are placed in plastic tubs. Towels are kept moist with tap water during the germination period. 
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ENO: 131 Helianthus (wilds) 
Duration 
Temperature 
Fluorescent Lights 
14 days 
4 ºC 
 
12 hours 
25 ºC 
ON 
12 hours 
15 ºC 
OFF 
Seeds are placed on top of 2 blue blotter papers moistened with distilled water in plastic boxes (5" x 51/4"). Prechill for 14 days. Transfer to germinator with temperatures alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle.
Rep and sample size depends on quantity of seed available: If > 2000 sds, then 4 reps of 50 sd for total of 200 seeds. If > 1500 & < 2000 , then 4 reps of 25 sd for total of 100. If < 1500, then do not run germination test.
Germination counts occur at 5 and 14 days after start of the germination test. 
TOP
ENO: 492632 Iberis 
Duration 
Temperature 
Fluorescent Lights 
14 days 
4 ºC 
 
12 hours 
25 ºC 
ON 
12 hours 
15 ºC 
OFF 
Seeds are placed on top of 2 blue blotter papers moistened with 0.1% KNO_{3} in plastic boxes (5" x 51/4") and kept at room temperature for 48 hours. Prechill at 4 ºC for 14 days. Transfer to germinator with temperatures alternating alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test. 
TOP
ENO: 493337 Lepidium

Duration 
Temperature 
Fluorescent Lights 
7 days 
4 ºC 
 
12 hours 
15 ºC 
ON 
12 hours 
15 ºC 
OFF 
Seeds are placed between 2 blue blotter papers moistened with 0.1% KNO_{3} solution in plastic boxes (5" x 51/4") and kept at room temperature overnight. Prechill at 4 ºC for 7 days. Transfer to germinator set at constant 15 ºC with light for 12 hours and dark for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur weekly after start of test. 
TOP
ENO: 490309 Linum (cultivated) 
Duration 
Temperature 
Fluorescent Lights 
12 hours 
30 ºC 
ON 
12 hours 
20 ºC 
OFF 
Seeds are placed on top of 2 blue papers moistened with tap water in plastic boxes (5" x 51/4"); let sit overnight at room temperature. Transfer to germinator with temperatures alternating alternating between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle.
Rep and sample size depends on quantity of seed available: If > 6000 sds, then 4 reps of 50 sd for total of 200 seeds. If > 2100 & < 6000 , then 4 reps of 25 sd for total of 100. If < 2100, then do not run germination test.
Germination count days also vary: If L. usitatissimum, then counts occur on 3 and 7 days after start of test. Otherwise, counts occur at 7, 10, and 14 days after start of test. 
TOP
ENO: 491459 Linum (wilds) 
Duration 
Temperature 
Fluorescent Lights 
12 hours 
15 ºC 
ON 
12 hours 
15 ºC 
OFF 
Seeds are placed on top of 2 blue papers moistened with 0.1% KNO_{3} in plastic boxes (5" x 51/4"); let sit overnight at room temperature. Transfer to germinator set at constant temperature of 15 ºC with light for 12 hours and darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 sd for total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of test.
If seeds do not germinate after 10 days, raise germination temperature to 20 ºC. 
TOP
ENO: 493622 Linum lewisii 
Duration 
Temperature 
Fluorescent Lights 
28 days 
4 ºC 
 
12 hours 
15 ºC 
ON 
12 hours 
15 ºC 
OFF 
Seeds are placed on top of 2 blue papers moistened with tap water in plastic boxes (5" x 51/4"); let sit overnight at room temperature. Prechill at 4 ºC for 28 days. Transfer to germinator set at constant temperature of 15 ºC with light for 12 hours and darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 sd for total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germiantion test.
If seeds do not germinate after 10 days, raise germination temperature to 20 ºC. 
TOP
ENO: 493809 Linum tenuifolium 
Duration 
Temperature 
Fluorescent Lights 
21 days 
4 ºC 
 
12 hours 
25 ºC 
ON 
12 hours 
15 ºC 
OFF 
Seeds are placed on top of 2 blue papers moistened with tap water in plastic boxes (5" x 51/4"); let sit overnight at room temperature. Prechill at 4 ºC for 21 days. Transfer to germinator with temperatures alternating alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 sd for total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test. 
TOP
ENO: 493336 Matthiola, Thlaspi 
Duration 
Temperature 
Fluorescent Lights 
7 days 
4 ºC 
 
12 hours 
30 ºC 
ON 
12 hours 
20 ºC 
OFF 
Seeds are placed on top of 2 blue blotter papers moistened with 0.1% KNO_{3} in plastic boxes (5" x 51/4"); let sit overnight at room temperature. Prechill at 4 ºC for 7 days. Transfer to germinator with temperatures alternating between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 7, 14, and 21 days after start of germination test. 
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