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United States Department of Agriculture

Agricultural Research Service

Germination Test Methods
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1 - Main area
2 - Amaranth: D. Brenner, Curator
3 - Maize: M. Millard, Curator
4 - Oilseeds: L. Marek, Curator
5 - Ornamentals: M. Widrlechner, Curator
6 - Vegetables: K. Reitsma, Curator
7 - Seed Testing Papers
8 - General & Historic
Amaranth: D. Brenner, Curator


Amaranth Curator Group logo

Click here for information on the seed testing papers we use.
Contact Curator David Brenner.

 

Amaranth

491828

Ferula

493851

Ammi

493850

Foeniculum

493814

Anethum

493834

Galega

493836

Angelica

493921

Grasses

490363

Anthriscus

493835

Levisticum

493851

Astrodaucus

490972

Melilotus

492586

Bifora

493836

Oreoschimperella

493840

Calamovilfa

152

Orlaya

493850

Caucalis

490972

Panicum

490363

Celosia

491462

Perilla

493899

Chenopodium
(cultivated)

491651

Petroselinum

493801

Chenopium
(wild)

493487

Setaria
(cultivated)

490361

Coniocelinum

493840

Setaria
(wild)

490385

Coriander

492498

Sium

493852

Cuminum

491828

Spinacia

491601

Dalea

493836

Umbels

490972

Echinochloa
(standard)
490362 Zizia 490972
Echinochloa
(side-by-side)
494560    

Eryngium

491625

 

 


ENO: 491828 Amaranth & Cuminum

Duration

Temperature

Fluorescent Lights

12 hours

30 ºC

ON

12 hours

20 ºC

OFF

Seeds are placed on top of 2 blue blotter papers moistened with tap water in plastic boxes (5" x 5-1/4"). Temperatures alternate between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: either 4 reps of 50 seed each or 2 reps of 100 seed each for a total of 200 seeds. The first count is done 3 or 4 days after start of test. If needed, second and third counts are done weekly after first count.

 TOP

ENO: 493850 Ammi & Orlaya

Duration

Temperature

Fluorescent Lights

7 days

10 ºC

---

12 hours

20 ºC

ON

12 hours

20 ºC

OFF

Seeds are placed on top of 3 blotter papers (1 blue, 2 white – in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight; prechill at 10 ºC for 7 days. Transfer to germinator. Temperature at a constant 20 ºC with light for 12 hours and dark for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. The counts are done 7 and 21 days after start of test.

 TOP

ENO: 493834 Anethum

Duration

Temperature

Fluorescent Lights

12 hours

30 ºC

ON

12 hours

20 ºC

OFF

Seeds are placed in folded paper towels moistened with tap water and placed in plastic tubs. These are kept at room temperature overnight and then transferred to a germinator. Temperatures alternate between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 7 and 21 days after start of test.

 TOP

ENO: 493921 Angelica

Duration

Temperature

Fluorescent Lights

14 days

4 ºC

---

12 hours

25 ºC

ON

12 hours

15 ºC

OFF

Seeds are placed on top of 2 blue blotter papers moistened with 1 mM GA3 in plastic boxes (5" x 5-1/4"); let sit overnight at room temperature. Prechill at 4 ºC for 14 days. Transfer to germinator with temperatures alternating alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur at 10, 17, and 28 days after start of the germination test.

 TOP

ENO: 493835 Anthriscus

Duration

Temperature

Fluorescent Lights

12 hours

30 ºC

ON

12 hours

20 ºC

OFF

Seeds are placed on top of 3 blotter papers (1 blue, 2 white – in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight. Transfer to germinator with temperatures alternating between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. The counts are done 7 and 21 days after start of test.

 TOP

ENO: 493836 Bifora, Dalea, & Galega

Duration

Temperature

Fluorescent Lights

10 days

4 ºC

---

12 hours

25 ºC

ON

12 hours

15 ºC

OFF

Seeds are placed in folded paper towels moistened with tap water and placed in plastic tubs. These are kept at room temperature overnight; prechill at 4 ºC for 10 days. Transfer to germinator. Temperatures alternate between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 7, 14, and 21 days after start of test. Chip any remaining ungerminated seed at 21 days and then extend test 5 more days. Do final count and end test.

 TOP

ENO: 152 Calamovilfa

Duration

Temperature

Fluorescent Lights

12 hours

30 ºC

ON

12 hours

20 ºC

OFF

Seeds are placed on top of 3 blotter papers (2 blue, 1 white – in contact with seeds) moistened with 0.1% KNO3 solution in plastic boxes (5" x 5-1/4"). Temperatures alternate between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 4 and 14 days after start of test.

 TOP

ENO: 491462 Celosia

Duration

Temperature

Fluorescent Lights

12 hours

30 ºC

ON

12 hours

20 ºC

OFF

Seeds are placed on top of 2 blue blotter papers moistened with tap water in plastic boxes (5" x 5-1/4"). Temperatures alternate between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. The first count is done 10 days after start of test. If needed, a second count is done 5 days after first count.

 TOP

ENO: 491651 Chenopodium (cultivated)

Duration

Temperature

Fluorescent Lights

12 hours

30 ºC

ON

12 hours

20 ºC

OFF

Seeds are placed on top of 3 blotter papers (2 blue, 1 white – in contact with seeds) moistened with 0.1% KNO3 solution in plastic boxes (5" x 5-1/4"). Temperatures alternate between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. The first count occurs 6 days after start of test.

 TOP

ENO: 493487 Chenopodium (dormant/wild species)

Duration

Temperature

Fluorescent Lights

1 month

4 ºC

---

12 hours

30 ºC

ON

12 hours

20 ºC

OFF

Seeds are placed on top of 3 blotter papers (2 blue, 1 white – in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4"). Leave at room temperature overnight; prechill at 4 ºC for one month. Transfer to germinator. Temperatures alternate between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. The first count occurs 6 days after transfer from cold treatment.


 TOP

ENO: 493840  Coniocelinum & Oreoschimperella

Duration

Temperature

Fluorescent Lights

12 hours

30 ºC

ON

12 hours

20 ºC

OFF

Seeds are placed between blotter papers moistened with tap water in plastic boxes (5" x 5-1/4") with the following arrangement:

(top) 1 blue paper
seeds
1 white paper
(bottom) 1 blue paper

Let seeds imbibe overnight at room temperature then place in germinator. Temperatures alternate between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 7 and 21 days after start of test.

 TOP


ENO: 492498 Coriander

Duration

Temperature

Fluorescent Lights

12 hours

15 ºC

ON

12 hours

15 ºC

OFF

Seeds are placed between blotter papers moistened with tap water in plastic boxes (5" x 5-1/4") with the following arrangement:

(top) 1 blue paper
seeds
1 white paper
(bottom) 1 blue paper

Temperature is constant at 15 ºC with light for 12 hours and darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. The first count occurs 6 days after start of test.

 TOP


ENO: 490362 Echinochloa (standard)

Duration

Temperature

Fluorescent Lights

12 hours

30 ºC

ON

12 hours

20 ºC

OFF

Seeds are placed on top of 2 blue blotter papers moistened with tap water in plastic boxes (5" x 5-1/4"). Temperatures alternate between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 4 and 10 days after start of test.

 TOP


ENO: 494560 Echinochloa (side-by-side)

Duration

Temperature

Fluorescent Lights

12 hours

30 ºC

ON

12 hours

20 ºC

OFF

There are two sets to this test:
    1) Light set: alternating light and dark, and
    2) Dark set: constant dark.

Germinations are conducted in blotter boxes.
Scoring is done on 7, 14, and 21 days after the start of germination.  IMPORTANT NOTE: First count is NOT to occur before 7 days.

If inventory on hand is > 6,000 seed, then:

    1) Light set:
           4 reps of 50 seed 
           30C + light (12hrs) / 20 + dark (12hrs)
           Put seed on top of blotter papers.

    2) Dark set:
           4 reps of 50 seed 
           Dark, 30C (12hrs) / 20C (12 hrs)
           Put seed in between dark, blue blotter papers
               (near center of papers),
           or cover boxes with aluminum foil.

If inventory on hand is 6,000 seed or less, then reduce sample size by conducting test on just 2 reps of 50 seed each..

 TOP


ENO: 491625 Eryngium

Duration

Temperature

Fluorescent Lights

12 hours

30 ºC

ON

12 hours

20 ºC

OFF

Seeds are placed on top of 3 blotter papers (2 blue, 1 white – in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4"). Leave at room temperature overnight. Transfer to germinator. Temperatures alternate between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 7 and 21 days after start of test.

 TOP

ENO: 493851 Ferula & Levisticum

Duration

Temperature

Fluorescent Lights

7 days

10 ºC

---

12 hours

25 ºC

ON

12 hours

15 ºC

OFF

Seeds are placed on top of 3 blotter papers (1 blue, 2 white – in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4") and kept at room temperature overnight; prechill at 10 ºC for 7 days. Transfer to germinator. Temperatures alternate between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. The counts are done 7 and 21 days after start of test.

 TOP


ENO: 493814 Foeniculum

Duration

Temperature

Fluorescent Lights

12 hours

30 ºC

ON

12 hours

20 ºC

OFF

Seeds are placed between blotter papers moistened with tap water in plastic boxes (5" x 5-1/4") with the following arrangement:

(top) 1 blue paper
seeds
1 white paper
(bottom) 1 blue paper

Let seeds imbibe overnight at room temperature then place in germinator. Temperatures alternate between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 7 and 14 days after start of test.

 TOP


ENO: 492586 Melilotus

Duration

Temperature

Fluorescent Lights

14 days

4 ºC

---

12 hours

20 ºC

ON

12 hours

20 ºC

OFF

Seeds are placed between blotter papers moistened with 0.1% KNO3 in plastic boxes (5" x 5-1/4") with the following arrangement:

(top) 1 blue paper
seeds
1 white paper
(bottom) 1 blue paper

Let seeds imbibe overnight at room temperature; prechill at 4 ºC for 14 days. Transfer to germinator. Temperature is constant at 20 ºC with light for 12 hours and darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 7 and 14 days after start of test.

 TOP


ENO: 490363 Panicum & Misc. Grasses

Duration

Temperature

Fluorescent Lights

7 days

4 ºC

---

12 hours

30 ºC

ON

12 hours

15 ºC

OFF

Seeds are placed on top of 2 blue blotter papers moistened with with 0.1% KNO3 solution in plastic boxes (5" x 5-1/4"). Leave at room temperature overnight; prechill at 4 ºC for 7 days. Transfer to germinator. Temperatures alternate between 30 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 7 and 21 days after start of test.

 TOP


ENO: 493899 Perilla

Duration

Temperature

Fluorescent Lights

12 hours

20 ºC

OFF

12 hours

20 ºC

OFF

Seeds are placed between 2 blotter papers moistened with tap water in plastic boxes (5" x 5-1/4") with the following arrangement:

(top) 1 white paper
seeds
(bottom) 1 white paper

Let seeds imbibe overnight at room temperature then place in germinator. Temperature is constant at 20 ºC with dark conditions. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 5 and 14 days after start of test.

 TOP


ENO: 493801 Petroselinum

Duration

Temperature

Fluorescent Lights

12 hours

30 ºC

ON

12 hours

20 ºC

OFF

Seeds are placed between blotter papers moistened with tap water in plastic boxes (5" x 5-1/4") with the following arrangement:

(top) 1 blue paper
seeds
1 white paper
(bottom) 1 blue paper

Let seeds imbibe overnight at room temperature then place in germinator. Temperatures alternate between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 7 and 14 days after start of test.

 TOP


ENO: 490361 Setaria (cultivated)

Duration

Temperature

Fluorescent Lights

12 hours

30 ºC

ON

12 hours

20 ºC

OFF

Seeds are placed in folded paper towels moistened with tap water and placed in plastic tubs. Temperatures alternate between 30 ºC with light for 12 hours and 20 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 4 and 10 days after start of test.

 TOP


ENO: 490385 Setaria (dormant/wild species)

Duration

Temperature

Fluorescent Lights

2 weeks

4 ºC

---

12 hours

30 ºC

ON

12 hours

15 ºC

OFF

Seeds are placed between blotter papers moistened with 0.1% KNO3 in plastic boxes (5" x 5-1/4") with the following arrangement:

(top) 1 blue paper
seeds
1 white paper
(bottom) 1 blue paper

Let seeds imbibe overnight at room temperature then place in 4 ºC for 2 weeks. Transfer to germinator. Temperatures alternate between 30 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur 7 and 21 days after start of test.

 TOP


ENO: 493852 Sium

Duration

Temperature

Fluorescent Lights

7 days

10 ºC

---

12 hours

25 ºC

ON

12 hours

15 ºC

OFF

Seeds are placed on top of 2 blue blotter papers moistened with tap water in plastic boxes (5" x 5-1/4"); make sure blotter paper is very wet. These are kept at room temperature overnight; prechill at 10 ºC for 7 days. Transfer to germinator. Temperatures alternate between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. The counts are done 7 and 21 days after start of test.

 TOP


ENO: 491601 Spinacia

Duration

Temperature

Fluorescent Lights

Until seeds sprout

5 ºC

---

12 hours

25 ºC

ON

12 hours

15 ºC

OFF

Seeds are placed on top of 3 blotter papers (2 blue, 1 white – in contact with seeds) moistened with tap water in plastic boxes (5" x 5-1/4"). The plastic boxes are wrapped with parafilm to help maintain moisture during the long cold treatment. Temperature is a constant 5 ºC until seeds begin to sprout. Transfer to germinator. Temperatures alternate between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Germination counts occur immediately and then 7, 14, 21, and 28 days after transfer from cold treatment.

 TOP

ENO: 490972 Umbels
(includes Astrodaucus, Caucalis & Zizia)

Duration

Temperature

Fluorescent Lights

12 hours

25 ºC

ON

12 hours

15 ºC

OFF

Seeds are placed in folded paper towels moistened with tap water and placed in plastic tubs. Let sit overnight at room temperature. Transfer to germinator with temperatures alternating between 25 ºC with light for 12 hours and 15 ºC in darkness for 12 hours per 24 hour cycle. Replication and sample size: 4 reps of 50 seed each for a total of 200 seeds. Counts are done at 7, 14, and 21 days after start of test.

 TOP

 

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Last Modified: 3/8/2012
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