WHNRC Nutritional Metabolomics Research Group Instrumentation
Waters Acquity UPLC - Applied Biosystems/Sciex API 4000 Q TRAP® MS/MS
The combined technology of ultra high performance liquid chromatography with sub-two micron separations and hybrid triple quadrupole/linear ion trap mass spectrometer gives us highly resolved, high-sensitivity full-scan MS, MS/MS, and MS3 with high-selectivity in precursor and neutral loss modes. Our current applications include targeted quantitative Oxylipin Metabolite Profiling of over 80 endogenous analytes coupled with non-targeted peak collection. Methods in development are targeted/non-target bile acid, endocanabinoid, and selective peptide marker metabolomic profiling.
Agilent 6890/5973 GC-MSD
The Agilent Technologies gas chromatograph coupled to their single-quad mass selective detector allows for selective and sensitive quantitative analysis of fatty acid methyl esters (FAME analysis) Our current FAME multi-residue analysis consists of 62 fatty acids ranging from C6:0 to C24:6N3.
Waters 600 Series FPLC System
A Waters 626 HPLC pump, 717 autosampler and 996 PDA detector are coupled to a chilled FRAC 200 to perform lipoprotein particle separation with a Superose 6 gel permeation (size exclusion) column. Fresh plasma samples are fractionated according to Scheffer (1997) and frozen immediately at 80ºC for compositional analysis.
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