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Bud Storage Process for Malus
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1. Single bud section 35 mm long with healthy bud being cut from dormant well acclimated scion. |
2. 10 bud segments being weighed prior to desiccation and samples placed on wire drying rack. |
3. Buds dried at -40°C in lab freezer modified with controller and heat tape. |
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4. Buds dried to 30% moisture and sealed in heat shrinkable tubes with birch plugs in both ends. |
5. Multiple tubes each containing 12 bud segments placed in aluminum cryo boxes. |
6. Boxes with buds frozen to -30°C in programmable freezer. |
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7. Boxes transferred to LN storage tank and held at almost -170 °C. |
8. 330 boxes can be stored in each LN storage tank. |
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Last Modified: 10/14/2011
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